褪黑素抑制H2O2诱导的人脐静脉内皮细胞氧化应激及凋亡标志蛋白质的表达

doi:10.16352/j.issn.1001-6325.2024.05.0645

基础医学与临床 ›› 2024, Vol. 44 ›› Issue (5): 645-650.doi: 10.16352/j.issn.1001-6325.2024.05.0645

褪黑素抑制H₂O₂诱导的人脐静脉内皮细胞氧化应激及凋亡标志蛋白质的表达

杨瀚毅^1,2#, 宁佳怡^1,2#, 汪小兰^2#, 张益萌², 谢婷珂^1,2, 陈奕玄^1,2, 韩静^2*

1.西安医学院眼科学教研室,陕西西安 710068;
2.空军军医大学第二附属医院眼科, 陕西西安 710038

收稿日期:2023-09-26 修回日期:2024-01-24 出版日期:2024-05-05 发布日期:2024-04-23
通讯作者: ^*hanjing.cn@163.com
^#对本文有相同贡献
基金资助:
国家自然科学基金 (8217111370);陕西省科技攻关重点研发计划 (2021SF-158);唐都医院临床重点研究 (2021LCYJ019,2018LCYJ008)

Melatonin inhibits H₂O₂-induced oxidative stress and apoptosis marker protein expression in human umbilical vein endothelial cells

YANG Hanyi^1,2#, NING Jiayi^1,2#, WANG Xiaolan^2#, ZHANG Yimeng², XIE Tingke^1,2, CHEN Yixuan^1,2, HAN Jing^2*

1. Department of Ophthalmology, Xi′an Medical University, Xi′an 710068;
2. Department of Ophthalmology, the Second Affiliated Hospital of the Air Force Medical University, Xi′an 710038, China

Received:2023-09-26 Revised:2024-01-24 Online:2024-05-05 Published:2024-04-23
Contact: ^*hanjing.cn@163.com

摘要/Abstract

摘要： 目的探讨褪黑素(MT)对过氧化氢诱导的人脐静脉内皮细胞(HUVECs)氧化应激机制与凋亡标志蛋白质的表达的影响。方法将HUVECs细胞分为空白组和H₂O₂(100、200、300、400、600和700 mmol/L)组,用CCK8法筛选出造模最佳浓度;为了明确MT对H₂O₂造成的氧化应激损伤的影响,将HUVECs细胞分为空白组、H₂O₂组和MT组。Western blot检测细胞中p-p65/p65、SOD2和cleaved-caspase 3的表达;试剂盒检测细胞中超氧化物歧化酶(SOD)活性、丙二醛(MDA)和过氧化氢酶(CAT)浓度;活性氧物质(ROS)染色检测HUVECs细胞内ROS浓度。结果相较于空白组,H₂O₂组细胞内的p-p65/p65和cleaved-caspase 3表达显著上升(P＜0.001),SOD2表达明显下降(P＜0.05);SOD活性(P＜0.001)和CAT浓度(P＜0.01)明显下降,MDA浓度明显上升(P＜0.001);ROS含量显著升高(P＜0.001)。相较于H₂O₂组,MT干预组细胞内的p-p65/p65(P＜0.001)和cleaved-caspase 3表达(P＜0.01)明显下调,SOD2表达明显增高(P＜0.001);并且SOD活性(P＜0.001)和CAT浓度上升(P＜0.05),MDA浓度下降(P＜0.001);从ROS染色的结果看来ROS含量有明显下降(P＜0.001)。结论 MT通过上调SOD2的表达、下调p-p65/p65和cleaved-caspase 3表达,发挥对H₂O₂介导的HUVECs氧化应激损伤的保护作用。

关键词: 生物医学工程, 褪黑素(MT), 分子生物学, 人脐静脉内皮细胞(HUVECs), 氧化应激

Abstract: Objective To investigate the effects of melatonin (MT) on oxidative stress and expression of apoptosis marker protein in human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide (H₂O₂). Methods HUVECs were divided into blank group and H₂O₂ (100, 200, 300, 400, 600, 700 mmol/L) groups.CCK8 method was used to select the best concentration for modeling. To clarify the effect of melatonin on oxidative stress injury caused by H₂O₂, HUVECs were divided into control group, H₂O₂ group and melatonin group. Western blot was used to detect the expression of p-p65/p65, SOD2 and cleaved-caspase 3. The activity of super-oxide dismutase (SOD), the concentration of malondialdehyde (MDA) and catalase (CAT) were measred by commercially available kits. Reactive oxygen species (ROS) staining was used to detect ROS content in HUVECs. Results H₂O₂ increased the expression of p-p65/p65 and cleaved-caspase 3(P＜0.001), decreased the expression of SOD2(P＜0.05)its biological activity(P＜0.001) and the concentration of CAT(P＜0.01), elevated the concentration of MDA(P＜0.001) and the level of ROS(P＜0.001)in HUVECs. Compared with H₂O₂ group, MT treatment decreased the expression of p-p65/p65(P＜0.001), SOD2(P＜0.001)and cleaved-caspase 3(P＜0.01), increased the expression of SOD2(P＜0.001)as well as biological activity of SOD(P＜0.001) and the concentration of CAT(P＜0.05) reduced the concentration of MDA(P＜0.001) and the level of ROS(P＜0.001)in HUVECs. Conclusions Melatonin plays a protective role in oxidative stress injury of HUVECs induced by H₂O₂ through up-regulating the expression of SOD2 and down-regulating the expression of p-p65/p65 and cleaved-caspase 3.

Key words: biomedical engineering, melatonin(MT), molecular biology, human umbilical vein endothelial cells (HUVECs), oxidative stress

中图分类号:

杨瀚毅, 宁佳怡, 汪小兰, 张益萌, 谢婷珂, 陈奕玄, 韩静. 褪黑素抑制H₂O₂诱导的人脐静脉内皮细胞氧化应激及凋亡标志蛋白质的表达[J]. 基础医学与临床, 2024, 44(5): 645-650.

YANG Hanyi, NING Jiayi, WANG Xiaolan, ZHANG Yimeng, XIE Tingke, CHEN Yixuan, HAN Jing. Melatonin inhibits H₂O₂-induced oxidative stress and apoptosis marker protein expression in human umbilical vein endothelial cells[J]. Basic & Clinical Medicine, 2024, 44(5): 645-650.

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褪黑素抑制H₂O₂诱导的人脐静脉内皮细胞氧化应激及凋亡标志蛋白质的表达

Melatonin inhibits H₂O₂-induced oxidative stress and apoptosis marker protein expression in human umbilical vein endothelial cells

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