基础医学与临床 ›› 2024, Vol. 44 ›› Issue (2): 147-153.doi: 10.16352/j.issn.1001-6325.2024.02.0147

• 研究论文 • 上一篇    下一篇

右美托咪定通过激活AMPK减轻TNF-α诱导人成神经细胞瘤细胞系SH-SY5Y损伤

牟杨1, 杨志文2*   

  1. 重庆大学附属涪陵医院 1.康复医学科; 2.急诊医学部,重庆 408099
  • 收稿日期:2023-07-31 修回日期:2023-11-27 出版日期:2024-02-05 发布日期:2024-02-05
  • 通讯作者: *840400498@qq.com
  • 基金资助:
    国家自然科学基金(81870883);重庆市区域医学重点学科(zdxk202110)

Dexmedetomidine alleviates TNF-α-induced injury of human neuroblastoma cell line SH-SY5Y through activating AMPK

MU Yang1, YANG Zhiwen2*   

  1. 1. Department of Rehabilitation Medicine; 2. Department of Emergency Medicine, Chongqing University Fuling Hospital,Chongqing 408099,China
  • Received:2023-07-31 Revised:2023-11-27 Online:2024-02-05 Published:2024-02-05
  • Contact: *840400498@qq.com

摘要: 目的 研究右美托咪定(DEX)对TNF-α诱导的人成神经细胞瘤细胞系的作用及其机制。方法 采用CCK8检测细胞活性,确定最佳DEX和TNF-α剂量,细胞分为:对照组(control组)、模型组(model组)、DEX干预模型组(DEX组)、DEX联合compound C干预模型组(DEX+CC组);Western blot检测细胞中p-AMPK、SNHP、KIF5B、Drp1、OPA1蛋白表达,ELISA检测IL-1β、IL-6水平,相应试剂盒测定线粒体膜电位(Δψm)、呼吸链复合酶活性(complex Ⅰ-Ⅳ)、ATP、MDA、SOD、GSH、ROS。结果 模型组较对照组 p-AMPK活性、OPA1及SNPH水平显著降低,但Drp1和KIF5B水平显著增高(P<0.01),DEX组较model组complex Ⅰ~Ⅳ及Δψm、ATP、GSH、SOD水平显著增高,而MDA、IL-1β、IL-6水平显著降低(P<0.01);DEX+CC组较DEX组complex Ⅰ~Ⅳ及Δψm、ATP、GSH、SOD水平显著降低,而MDA、IL-1β、IL-6水平显著增高(P<0.01)。结论 DEX通过依赖AMPK的方式改善线粒体功能、减少氧化应激及炎性反应,减轻TNF-α诱导的细胞损伤。

关键词: 右美托咪定, 氧化应激, 腺苷酸活化蛋白激酶(AMPK)

Abstract: Objective To study the effect of dexmedetomidine (DEX) on TNF-α-induced injury of human neuroblastoma cell line SH-SY5Y and its mechanism. Methods CCK8 assay was used to detect cell activity and determine the optimal dose of DEX and TNF-α. The cells were divided into control group, model group, DEX intervention model group, and the intervention group of DEX plus compound C. Western blot was used to detect the expression of p-AMPK, SNHP, KIF5B, Drp1 and OPA1, and ELISA was used to detect the level of IL-1β and IL-6. Mitochondrial membrane potential (Δψm), respiratory chain complex enzyme activity (complex Ⅰ-Ⅳ), ATP, MDA, SOD, GSH, ROS were determined with commercially available kits. Results Compared with control group, level of p-AMPK,OPA1 and SNPH l in model group significantly decreased, while the level of Drp1 and KIF5B significantly increased (P<0.01). The level of complex Ⅰ~Ⅳ, Δψm, ATP, GSH and SOD in DEX group significantly increased as compared with model group. The level of MDA, IL-1β and IL-6 significantly decreased(P<0.01). Compared with DEX group, the level of Complex Ⅰ-Ⅳ, Δψm, ATP, GSH and SOD in DEX+CC group significantly decreased, while the level of MDA, IL-1β and IL-6 significantly increased(P<0.01). Conclusions DEX improves mitochondrial function, alleviates oxidative stress and inflammatory response as well as TNF-α-induced cell damage with an AMPK dependent mechanism.

Key words: dexmedetomidine, oxidative stress, adenylate activates protein kinase (AMPK)

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