LncRNA UNC5B-AS1靶向miR-339-5p调控人肺腺癌细胞系A549增殖和凋亡

doi:10.16352/j.issn.1001-6325.2022.03.022

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (3): 454-460.doi: 10.16352/j.issn.1001-6325.2022.03.022

LncRNA UNC5B-AS1靶向miR-339-5p调控人肺腺癌细胞系A549增殖和凋亡

张健, 张吉炯^*

黄石市中心医院呼吸内科, 湖北黄石 435000

收稿日期:2021-01-19 修回日期:2021-08-06 出版日期:2022-03-05 发布日期:2022-03-04
通讯作者: ^* 645644134@qq.com
基金资助:
湖北省卫生计生委项目(WJ2018H0115)

LncRNA UNC5B-AS1 targeting miR-339-5p regulates the proliferation and apoptosis of human lung adenocarcinoma cell line A549

ZHANG Jian, ZHANG Ji-jiong^*

Department of Respiratory Medicine, Huangshi Central Hospital, Huangshi 435000, China

Received:2021-01-19 Revised:2021-08-06 Online:2022-03-05 Published:2022-03-04
Contact: ^* 645644134@qq.com

摘要/Abstract

摘要： 目的旨在研究lncRNA UNC5B-AS1靶向miR-339-5p对肺癌细胞增殖和凋亡的影响及分子机制。方法体外培养人肺腺癌细胞系A549;将si-NC、si-UNC5B-AS1、miR-NC、miR-339-5p mimics、si-UNC5B-AS1与anti-miR-NC、si-UNC5B-AS1与anti-miR-339-5p分别转染至A549细胞。RT-qPCR检测肺癌组织和癌旁组织中UNC5B-AS1和miR-339-5p表达;CCK-8法检测细胞增殖;流式细胞测量术检测细胞凋亡;双荧光素酶报告基因实验检测UNC5B-AS1与miR-339-5p的靶向调控关系;Western blot检测细胞周期蛋白D1(cyclin D1)、p21蛋白、B淋巴细胞瘤-2 (Bcl-2)、B淋巴细胞瘤-2相关蛋白(Bax)表达。结果与癌旁组织比较,肺癌组织中UNC5B-AS1表达水平显著升高(P＜0.05),miR-339-5p表达水平显著降低(P＜0.05)。与si-NC组比较,si-UNC5B-AS1组细胞活力显著降低(P＜0.05),细胞凋亡率显著升高(P＜0.05)。与miR-NC组比较,miR-339-5p组细胞活力显著降低(P＜0.05),细胞凋亡率显著升高(P＜0.05)。与(si-UNC5B-AS1+anti-miR-NC)组比较,(si-UNC5B-AS1+anti-miR-339-5p)组细胞活力显著升高(P＜0.05);细胞凋亡率显著降低(P＜0.05)。结论通过干扰UNC5B-AS1上调人肺腺癌细胞系A549的miR-339-5p表达,促进其凋亡、抑制其增殖。

关键词: lncRNA UNC5B-AS1, miR-339-5p, 肺癌, 增殖, 凋亡

Abstract: Objective To study the effect of lncRNA UNC5B-AS1 targeting miR-339-5p on the proliferation and apoptosis of lung cancer cells and its molecular mechanism. Methods The human lung adenocarcinoma cell line A549 was cultured in vitro, and si-NC, si-UNC5B-AS1, miR-NC, miR-339-5p mimics, si-UNC5B-AS1 and anti-miR-NC, si-UNC5B-AS1 and anti-miR-339-5p were transfected into A549 cells respectively. Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of UNC5B-AS1 and miR-339-5p in lung cancer tissues and in adjacent tissues; Cell counting kit (CCK-8) was used to detecte cell proliferation; The targeted regulation relationship between UNC5B-AS1 and miR-339-5p was examined by dual luciferase reporter gene experiment; Western blot was used to detect the expression of cyclin D1 (cyclin D1), p21 protein lymphoma-2(Bcl-2), and B lymphoma-2 related protein (Bax). Results Compared with adjacent tissues, the expression level of UNC5B-AS1 in lung cancer tissue was significantly increased (P＜0.05), and the expression level of miR-339-5p was significantly decreased(P＜0.05). Compared with the si-NC group, the cell viability of the si-UNC5B-AS1 group was significantly reduced (P＜0.05), the apoptosis rate was significantly higher(P＜0.05). Compared with the miR-NC group, the cell viability of the miR-339-5p group was significantly reduced (P＜0.05), and the apoptosis rate was significantly increased(P＜0.05). Compared with the (si-UNC5B-AS1+anti-miR-NC)group, the cell viability of the (si-UNC5B-AS1+anti-miR-339-5p) group was significantly increased (P＜0.05); the apoptosis rate was significantly reduced (P＜0.05). Conclusions Interference with UNC5B-AS1 promotes apoptosis and inhibits cell proliferation of human lung adenocarcinoma cancer cell line A549 by up-regulating the expression of miR-339-5p.

Key words: lncRNA UNC5B-AS1, miR-339-5p, lung cancer, proliferation, apoptosis

中图分类号:

R734.2

张健, 张吉炯. LncRNA UNC5B-AS1靶向miR-339-5p调控人肺腺癌细胞系A549增殖和凋亡[J]. 基础医学与临床, 2022, 42(3): 454-460.

ZHANG Jian, ZHANG Ji-jiong. LncRNA UNC5B-AS1 targeting miR-339-5p regulates the proliferation and apoptosis of human lung adenocarcinoma cell line A549[J]. Basic & Clinical Medicine, 2022, 42(3): 454-460.

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LncRNA UNC5B-AS1靶向miR-339-5p调控人肺腺癌细胞系A549增殖和凋亡

LncRNA UNC5B-AS1 targeting miR-339-5p regulates the proliferation and apoptosis of human lung adenocarcinoma cell line A549

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