萝卜硫素减轻心脏移植模型大鼠缺血/再灌注损伤

doi:10.16352/j.issn.1001-6325.2022.03.029

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (3): 411-416.doi: 10.16352/j.issn.1001-6325.2022.03.029

萝卜硫素减轻心脏移植模型大鼠缺血/再灌注损伤

王帅^1*, 王建军², 孙秀红¹, 邬鹏宇¹, 崔志成¹, 李占清¹

华北理工大学附属医院 1.心脏血管外科;
2.重症医学科(ICU),河北唐山 063000

收稿日期:2020-10-15 修回日期:2021-07-02 出版日期:2022-03-05 发布日期:2022-03-04
通讯作者: ^* j8pjcg@163.com
基金资助:
河北省中医药管理局科研计划项目(2018184)

Sulforaphane alleviates ischemia- reperfusion injury of heart transplantation in rat models

WANG Shuai^1*, WANG Jian-jun², SUN Xiu-hong¹, WU Peng-yu¹, CUI Zhi-cheng¹, LI Zhan-qing¹

1. Department of Cardiovascular Surgery;
2. Department of Critical Care Medicine(ICU), the Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China

Received:2020-10-15 Revised:2021-07-02 Online:2022-03-05 Published:2022-03-04
Contact: ^* j8pjcg@163.com

摘要/Abstract

摘要： 目的研究萝卜硫素在大鼠心脏移植缺血/再灌注损伤(I/RI)中的作用及PI3K/AKT/GSK-3β通路是否参与其中的作用。方法构建大鼠同种异体活体心脏移植模型为对照组,尾静注射萝卜硫素为萝卜磁素处理组(2.5 mg/kg)。比较大鼠的存活率、血清心肌酶、心肌细胞凋亡水平及心肌线粒体的肿胀程度;Western blot检测凋亡相关分子caspase-3、Bax和PI3K/AKT/GSK-3β的表达。结果萝卜硫素处理组的生存率显著高于对照组(P＜0.05);萝卜硫素处理组不同处理时间(6、12、24及48 h)血清乳酸脱氢酶(LDH)、肌酸肌酶(CK)、肌酸肌酶同工酶(CK-MB)和肌钙蛋白T(TnT)的浓度均呈时间依赖性低于对照组。Caspase-3和Bax的表达明显降低(P＜0.01),线粒体肿胀度也较对照组有所改善。结论萝卜硫素可能通过激活PI3K/Akt/GSK-3β通路抑制心肌细胞凋亡,改善大鼠心脏移植术后的I/RI。

关键词: 萝卜硫素, 心脏移植, 凋亡, 缺血/再灌注损伤

Abstract: Objective To investigate the effect of sulforaphane on ischemia-reperfusion injury(I/RI) and the role of PI3K/Akt/GSK-3β pathway in heart transplantation of rats. Methods The rat model of allograft heart transplantation was established as the control group.The survival rate,the level of serum myocardial enzymes,apoptosis of myocardial cells and the swelling degree of myocardial mitochondria were compared with sulforaphane injection group (2.5 mg/kg).Western blot was used to detect the expression of apoptotic molecules caspase-3,Bax and PI3K/Akt/GSK-3β. Results The survival rate of sulforaphane group was significantly higher than that of control group(P＜0.05).The serum concentrations of LDH,CK,CK-MB and TNT in sulforaphane group were lower than those in control group with a time dependent manner at different treatment time (6,12,24 and 48 h).The expressions of caspase-3 and Bax were significantly decreased (P＜0.01),and the degree of mitochondrial swelling was also improved compared with the control group. Conclusions Sulforaphane may inhibit myocardial apoptosis by activating PI3K/Akt/GSK-3β pathway and improve I/RI after heart transplantation as shown by animal model.

Key words: sulforaphane, heart transplantation, apoptosis, ischemia-reperfusion injury

中图分类号:

R541.8

王帅, 王建军, 孙秀红, 邬鹏宇, 崔志成, 李占清. 萝卜硫素减轻心脏移植模型大鼠缺血/再灌注损伤[J]. 基础医学与临床, 2022, 42(3): 411-416.

WANG Shuai, WANG Jian-jun, SUN Xiu-hong, WU Peng-yu, CUI Zhi-cheng, LI Zhan-qing. Sulforaphane alleviates ischemia- reperfusion injury of heart transplantation in rat models[J]. Basic & Clinical Medicine, 2022, 42(3): 411-416.

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萝卜硫素减轻心脏移植模型大鼠缺血/再灌注损伤

Sulforaphane alleviates ischemia- reperfusion injury of heart transplantation in rat models

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