绞股蓝皂苷抑制人前列腺癌细胞系PC-3增殖、迁移和侵袭

doi:10.16352/j.issn.1001-6325.2025.08.1059

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (8): 1059-1065.doi: 10.16352/j.issn.1001-6325.2025.08.1059

绞股蓝皂苷抑制人前列腺癌细胞系PC-3增殖、迁移和侵袭

邹庆波, 张国臣, 潘长景^*

济南市中西医结合医院泌尿外科,山东济南 271100

收稿日期:2024-06-21 修回日期:2025-01-08 出版日期:2025-08-05 发布日期:2025-07-11
通讯作者: ^*pchjpchj@163.com
基金资助:
济南市卫生健康委员会科技计划(2023-中-40)

Gypenoside inhibits the proliferation, migration and invasion of prostate cancer cell line PC-3

ZOU Qingbo, ZHANG Guochen, PAN Changjing^*

Department of Urology, Jinan Integrated Traditional Chinese Medicine and Western Medicine Hospital, Jinan 271100, China

Received:2024-06-21 Revised:2025-01-08 Online:2025-08-05 Published:2025-07-11
Contact: ^*pchjpchj@163.com

摘要/Abstract

摘要： 目的探讨绞股蓝皂苷(Gyp)对人前列腺癌细胞系(PC-3)增殖、迁移和侵袭的影响。方法使用200~1 200 μg/mL的Gyp处理PC-3细胞,筛选最佳药物浓度;将PC-3细胞分为对照组、Gyp-L组(400 μg/mL)、Gyp-M组(600 μg/mL)和Gyp-H组(800 μg/mL)、Gyp-H+8-溴-cAMP组(100 μmol/L PKA激活剂)。细胞增殖用集落形成实验检测;细胞凋亡用流式细胞仪检测;细胞迁移能力用划痕实验检测;细胞侵袭能力用Transwell法检测;cAMP水平用ELISA实验检测;E-cadherin、N-cadherin、Snail、PKA、CREB蛋白表达用Western blot检测。结果 Gyp呈浓度依赖性抑制PC-3细胞增殖,选择浓度为400、600、800 μg/mL的Gyp进行后续实验;与对照组比较,Gyp-L组、Gyp-M组、Gyp-H组集落形成数目、侵袭细胞数、划痕愈合率、Snail、N-cadherin、PKA、cAMP、CREB蛋白表达降低(P＜0.05),细胞凋亡率、E-cadherin蛋白表达升高(P＜0.05);PKA激活剂可减弱Gyp对前列腺癌细胞的恶性生物学行为的抑制作用。结论 Gyp可抑制前列腺癌细胞系PC-3增殖、迁移和侵袭,促进前列腺癌细胞系PC-3凋亡。

关键词: 绞股蓝皂苷, 前列腺癌, 增殖, 迁移

Abstract: Objective To investigate the effects of gypenoside(Gyp) on proliferation, migration and invasion of human prostate cancer cell line PC-3. Methods PC-3 cells were treated with 200-1 200 μg/mL Gyp to determine the optimal concentration. PC-3 cells were divided into control, Gyp-L group(400 μg/mL), Gyp-M group(600 μg/mL) and Gyp-H group(800 μg/mL), and Gyp-H+8-bromo-cAMP group(100 μmol/L PKA activator). Colony formation assay was applied to detect cell proliferation. Apoptosis was measured by flow cytometry. Scratch experiment was applied to detect the migration ability. Transwell assay was applied to detect the invasive ability. ELISA experiment was applied to detect cAMP level in each group. Western blot was applied to detect the expression of E-cadherin, N-cadherin, Snail, PKA and CREB proteins. Results Gyp inhibited PC-3 proliferation in a concentration dependent manner. Gyp concentration of 400, 600 and 800 μg/mL was selected for subsequent experiments. Compared with the control group, colony formation, number of invasive cells, scratch healing rate, the levels of Snail,N-cadherin, PKA, cAMP, and CREB proteins in the Gyp-L, Gyp-M, and Gyp-H groups were significantly reduced (P＜0.05),but the apoptosis rate as well as the level of E-cadherin protein were significantly increased(P＜0.05). PKA activator attenuated the inhibitory effect of Gyp on the malignant behavior of prostate cancer cells. Conclusions Gyp inhibits proliferation, migration and invasion, and promotes apoptosis in prostate cancer cell line PC-3.

Key words: gypenoside, prostate cancer, proliferation, migration

中图分类号:

R737.25

邹庆波, 张国臣, 潘长景. 绞股蓝皂苷抑制人前列腺癌细胞系PC-3增殖、迁移和侵袭[J]. 基础医学与临床, 2025, 45(8): 1059-1065.

ZOU Qingbo, ZHANG Guochen, PAN Changjing. Gypenoside inhibits the proliferation, migration and invasion of prostate cancer cell line PC-3[J]. Basic & Clinical Medicine, 2025, 45(8): 1059-1065.

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绞股蓝皂苷抑制人前列腺癌细胞系PC-3增殖、迁移和侵袭

Gypenoside inhibits the proliferation, migration and invasion of prostate cancer cell line PC-3

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