小檗碱增强前列腺癌细胞系22RV1对多西他赛的化疗敏感性

doi:10.16352/j.issn.1001-6325.2026.01.0039

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (1): 39-45.doi: 10.16352/j.issn.1001-6325.2026.01.0039

小檗碱增强前列腺癌细胞系22RV1对多西他赛的化疗敏感性

陆巍^1*, 王焱旻¹, 刘炳辰¹, 陶欣¹, 王世鹏¹, 曹秋也²

1.吉林省人民医院泌尿外科,吉林长春 130021;
2.乾安县人民医院胸外泌尿科,吉林松原 131499

收稿日期:2025-01-07 修回日期:2025-04-28 出版日期:2026-01-05 发布日期:2025-12-29
通讯作者: ^*wkkki2@163.com
基金资助:
吉林省卫生健康科技能力提升项目(2021JC055)

Berberine enhances the chemotherapy sensitivity of prostate cancer cell line 22RV1 to docetaxel

LU Wei^1*, WANG Yanmin¹, LIU Bingchen¹, TAO Xin¹, WANG Shipeng¹, CAO Qiuye²

1. Department of Urology, Jilin Provincial People's Hospital, Changchun 130021;
2. Department of Thoracic and Urology, Qian'an County People's Hospital, Songyuan 131499, China

Received:2025-01-07 Revised:2025-04-28 Online:2026-01-05 Published:2025-12-29
Contact: ^*wkkki2@163.com

摘要/Abstract

摘要： 目的探讨小檗碱(BBR)增强前列腺癌细胞系22RV1对多西他赛(DTX)化学治疗(化疗)敏感性的影响。方法利用前列腺癌细胞系22RV1细胞构建相应的DTX耐药细胞。将成功建立的耐药细胞株(22RV1/DTX)分为:22RV1/DTX组、低浓度(L)-BBR+22RV1/DTX组、高浓度(H)-BBR+22RV1/DTX组、H-BBR+ERK激活剂+22RV1/DTX组;CCK8法检测细胞增殖;划痕愈合试验检测迁移;Transwell小室法检测侵袭;流式细胞测量术检测凋亡;Western blot检测细胞中细胞外调节蛋白激酶(ERK)、pERK、ETS转录因子(ELK1)蛋白表达。结果与22RV1/DTX组相比,L-BBR+22RV1/DTX组和H-BBR+22RV1/DTX组细胞的增殖、迁移、侵袭能力降低,凋亡率提高(P＜0.05);与H-BBR+22RV1/DTX组相比,H-BBR+ERK激活剂+22RV1/DTX组细胞的增殖、迁移、侵袭能力提高,凋亡率降低(P＜0.05);BBR可显著降低22RV1/DTX细胞pERK/ERK比值和ELK1的蛋白表达水平(P＜0.05),且其对22RV1/DTX细胞及通路的影响可被ERK激活剂逆转(P＜0.05)。结论 BBR可能通过抑制ERK/ELK1信号通路,抑制耐药细胞增殖、迁移、侵袭,促进细胞凋亡,增强前列腺癌细胞对DTX的化疗敏感性。

关键词: 小檗碱, ERK/ELK1, 前列腺癌细胞, 化疗敏感性

Abstract: Objective To investigate the chemotherapy effectiveness of berberine (BBR) to enhance sensitivity of prostate cancer cell line 22RV1 to docetaxel. Methods The docetaxel (DTX) resistant cell was constructed using prostate cancer cell line 22RV1. The drug-resistant cell strain(22RV1/DTX) was divided into: 22RV1/DTX group, low concentration (L)-BBB+22RV1/DTX group, high concentration (H)-BBB+22RV1/DTX group and H-BBR+ERK activator+22RV1/DTX group. CCK8 method was applied to detect cell proliferation. Scratch healing test was applied to detect migration. Transwell test was applied to detect invasion. Flow cytometry was applied to detect apoptosis. Western blot was applied to detect the expression of extra-cellular regulated protein kinases (ERK), pERK, and ETS transcription factor (ELK1) proteins in cells. Results Compared with 22RV1/DTX group, the proliferation, migration, and invasion of cells in the L-BBR+22RV1/DTX group and H-BBR+22RV1/DTX group were lower but the apoptosis rate was higher (P＜0.05). The proliferation, migration, and invasion of cells in the H-BBR+ERK activator+22RV1/DTX group were higher, and the apoptosis rate was lower (P＜0.05). BBR significantly reduced the pERK/ERK ratio and ELK1 protein expression level in 22RV1/DTX cells(P＜0.05).The effects on 22RV1/DTX cells and pathways were reversed by ERK activator (P＜0.05). Conclusions BBR may inhibit the proliferation, migration, and invasion of drug-resistant cells, promote cell apoptosis and thus enhance the chemotherapy sensitivity of prostate cancer cells to DTX with underlying mechanism of suppressing the ERK/ELK1 signaling pathway.

Key words: berberine, ERK/ELK1, prostate cancer cell, chemotherapy sensitivity

中图分类号:

R737.25

陆巍, 王焱旻, 刘炳辰, 陶欣, 王世鹏, 曹秋也. 小檗碱增强前列腺癌细胞系22RV1对多西他赛的化疗敏感性[J]. 基础医学与临床, 2026, 46(1): 39-45.

LU Wei, WANG Yanmin, LIU Bingchen, TAO Xin, WANG Shipeng, CAO Qiuye. Berberine enhances the chemotherapy sensitivity of prostate cancer cell line 22RV1 to docetaxel[J]. Basic & Clinical Medicine, 2026, 46(1): 39-45.

参考文献

[1]Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71:209-249. doi: 10.3322/caac.21660.
[2]Kim TJ, Lee YH, Koo KC. Current status and future perspectives of androgen receptor inhibition therapy for prostate cancer: a comprehensive review[J]. Biomolecules, 2021, 11:1-14. doi: 10.3390/biom11040492.
[3]Rauf A, Abu-Izneid T, Khalil AA, et al. Berberine as a potential anticancer agent: a comprehensive review[J]. Molecules, 2021, 26:1-19. doi: 10.3390/molecules26237368.
[4]Song D, Hao J, Fan D. Biological properties and clinical applications of berberine[J]. Front Med, 2020, 14:564-582. doi: 10.1007/s11684-019-0724-6.
[5]Clark A, Villarreal MR, Huang SB, et al. Targeting S6K/NFκB/SQSTM1/Polθ signaling to suppress radiation resistance in prostate cancer[J]. Cancer Lett, 2024, 597:1-15. doi: 10.1016/j.canlet.2024.217063.
[6]Montagnani V, Maresca L, Apollo A, et al. E3 ubiquitin ligase PARK2, an inhibitor of melanoma cell growth, is repressed by the oncogenic ERK1/2-ELK1 transcriptional axis[J]. J Biol Chem, 2020, 295:16058-16071. doi: 10.1074/jbc.RA120.014615.
[7]Xie W, Li S, Guo H, et al. Androgen receptor knockdown enhances prostate cancer chemosensitivity by down-regulating FEN1 through the ERK/ELK1 signalling pathway[J]. Cancer Med, 2023, 12:15317-15336. doi: 10.1002/cam4.6188.
[8]Xing Z, Li S, Xing J, et al. Silencing of LINC01963 enhances the chemosensitivity of prostate cancer cells to docetaxel by targeting the miR-216b-5p/TrkB axis[J]. Lab Invest, 2022, 102:602-612. doi: 10.1038/s41374-022-00736-4.
[9]Feng Q, Yang P, Wang H, et al. ID09, a newly-designed tubulin inhibitor, regulating the proliferation, migration, EMT process and apoptosis of oral squamous cell carcinoma[J]. Int J Biol Sci, 2022, 18:473-490. doi: 10.7150/ijbs.65824.
[10]Zhu C, Li K, Peng XX, et al. Berberine a traditional Chinese drug repurposing: Its actions in inflammation-associated ulcerative colitis and cancer therapy[J]. Front Immunol, 2022, 13:1-7. doi: 10.3389/fimmu.2022.1083788.
[11]Xiong RG, Huang SY, Wu SX, et al. Anticancer effects and mechanisms of berberine from medicinal herbs: an update review[J]. Molecules, 2022, 27:1-19. doi: 10.3390/molecules27144523.
[12]Li P, Shi Y, Gao D, et al. ELK1-mediated YTHDF1 drives prostate cancer progression by facilitating the translation of Polo-like kinase 1 in an m6A dependent manner[J]. Int J Biol Sci, 2022, 18:6145-6162. doi: 10.7150/ijbs.75063.
[13]Thiel G, Rössler OG. Glucose homeostasis and pancreatic islet size are regulated by the transcription factors Elk-1 and Egr-1 and the protein phosphatase calcineurin[J]. Int J Mol Sci, 2023, 24:1-12. doi: 10.3390/ijms24010815.
[14]Thiel G, Backes TM, Guethlein LA, et al. Critical protein-protein interactions determine the biological activity of Elk-1, a master regulator of stimulus-induced gene transcription[J]. Molecules, 2021, 26:1-17. doi: 10.3390/molecules26206125.

小檗碱增强前列腺癌细胞系22RV1对多西他赛的化疗敏感性

Berberine enhances the chemotherapy sensitivity of prostate cancer cell line 22RV1 to docetaxel

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

[1]	谢丹丹, 李含, 王白菊, 王娜, 陈汉文, 刘雷. 小檗碱减轻慢性肾衰竭大鼠的肾脏损伤[J]. 基础医学与临床, 2024, 44(12): 1663-1669.
[2]	刘琴, 杨黎星, 石婧, 黄雨晴, 高洪婷, 鞠瑞, 郭磊. 小檗碱激活巨噬细胞自噬并改善动脉粥样硬化斑块脆弱性[J]. 基础医学与临床, 2020, 40(5): 668-677.
[3]	于立明段维勋赵国龙张秋芳王晓武金振晓俞世强. 小檗碱减轻大鼠心肌缺血再灌注损伤[J]. 基础医学与临床, 2016, 36(4): 433-438.
[4]	李晶华王伟娟高婉丽郭蕾冯力民. HPV18 E6 siRNA对宫颈癌细胞增殖及化疗药物敏感性的影响[J]. 基础医学与临床, 2010, 30(5): 500-504.