NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤

doi:10.16352/j.issn.1001-6325.2025.08.1010

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (8): 1010-1015.doi: 10.16352/j.issn.1001-6325.2025.08.1010

NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤

计泽朝, 张琪, 阿不都许库尔·阿不力米提^*

新疆医科大学基础医学院组织与胚胎学教研室,新疆乌鲁木齐 830000

收稿日期:2024-09-26 修回日期:2024-12-31 出版日期:2025-08-05 发布日期:2025-07-11
通讯作者: ^*806613359@qq.com
基金资助:
国家自然科学基金(32060158)

NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B

JI Zezhao, ZHANG Qi, Abuduxukuer·ABULIMITI^*

Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830000, China

Received:2024-09-26 Revised:2024-12-31 Online:2025-08-05 Published:2025-07-11
Contact: ^*806613359@qq.com

摘要/Abstract

摘要： 目的研究活性氧(ROS)和细胞焦亡在胺碘酮诱导的肺上皮细胞损伤中的作用机制。方法 MTT法检测胺碘酮、胺碘酮+乙酰半胱氨酸(ROS抑制剂,NAC)、胺碘酮+NF-κB抑制剂组细胞存活率,DCFA探针检测活性氧水平,ELISA检测上清液中IL-6、IL-18、IL-1β、TNF-α水平,Western Blot检测NLRP3、Caspase-1 p20、GSDMD-N蛋白水平。结果胺碘酮浓度依赖性降低细胞存活率(P＜0.05),NAC和NF-κB抑制剂减轻胺碘酮的细胞毒性(P＜0.05)。胺碘酮诱导肺上皮细胞中ROS、炎性因子、焦亡蛋白的水平上升(P＜0.05),而NAC和NF-κB抑制剂可以降低这些指标的水平(P＜0.05)。结论 NF-κB抑制剂或NAC减轻胺碘酮体外诱导肺上皮细胞损伤。

关键词: 胺碘酮, 活性氧, 细胞损伤, NF-κB

Abstract: Objective To investigate the mechanism of reactive oxygen species (ROS) and pyroptosis in amiodarone-induced lung epithelial cell injury. Methods The viability of cells treated with amiodarone, amiodarone+N-acetylcysteine(ROS inhibitor,NAC) and amiodarone+NF-κB inhibitors was detected by MTT assay. ROS level was detected by DCFA probe and the levels of IL-6, IL-18, IL-1β and TNF-α in the supernatant were detected by ELISA; NLRP3. Caspase-1 p20 and GSDMD-N protein were detected by Western blot. Results Amiodarone concentration-dependently reduced cell viability(P＜0.05), NAC and NF-κB attenuated the cytotoxicity of amiodarone(P＜0.05). Amiodarone increased levels of reactive oxygen species, inflammatory factors and pyroptosis-related proteins in lung epithelial cells(P＜0.05), while NAC and NF-κB inhibitors reduced the levels of these indicators(P＜0.05). Conclusions NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line in vitro.

Key words: amiodarone, reactive oxygen species, cell injury, NF-κB

中图分类号:

计泽朝, 张琪, 阿不都许库尔·阿不力米提. NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤[J]. 基础医学与临床, 2025, 45(8): 1010-1015.

JI Zezhao, ZHANG Qi, Abuduxukuer·ABULIMITI. NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B[J]. Basic & Clinical Medicine, 2025, 45(8): 1010-1015.

参考文献 15

[1]	陆宇华, 赵婷婷, 张彤彤, 等. 胺碘酮临床不良反应及其预防措施的研究进展[J]. 中国药物滥用防治杂志, 2022,28:409-412.
[2]	Feduska ET, Thoma BN, Torjman MC, et al. Acute Amiodarone Pulmonary Toxicity[J]. J Cardiothorac Vasc Anesth, 2021,35:1485-1494.
[3]	Karinauske E, Abramavicius S, Musteikiene G, et al. A case report and literature review: previously excluded tuberculosis masked by amiodarone induced lung injury[J]. BMC Pharmacol Toxicol, 2018,19:88.doi: 10.1186/s40360-018-0279-1.
[4]	赵紫琴, 董淑慧, 尹海波, 等. siRNA沉默锌指蛋白A20基因促进人类风湿关节炎成纤维细胞样滑膜细胞焦亡[J]. 基础医学与临床, 2024,44:1407-1413.
[5]	陈才伟, 吴月平, 王转锁, 等. 大豆苷元减轻高糖诱导的肾小管上皮细胞焦亡[J]. 基础医学与临床, 2023,43:1801-1807.
[6]	Luo B, Huang F, Liu Y, et al. NLRP3 inflammasome as a molecular marker in diabetic cardiomyopathy[J]. Front Physiol, 2017,8:519.doi: 10.3389/fphys.2017.00519.
[7]	Luo T, Zhou X, Qin M, et al. Corilagin restrains NLRP3 inflammasome activation and pyroptosis through the ROS/TXNIP/NLRP3 pathway to prevent inflammation[J]. Oxid Med Cell Longev, 2022,2022:1652244.doi: 10.1155/2022/1652244.
[8]	Calabriso N, Massaro M, Scoditti E, et al. Epigenetic mechanisms in vascular inflammation: modulation of endothelial adhesion molecules and endothelium-leukocyte adhesion[J]. Front Biosci (Landmark Ed), 2023,28:194.doi: 10.31083/j.fbl2809194.
[9]	Mangan M, Olhava EJ, Roush WR, et al. Targeting the NLRP3 inflammasome in inflammatory diseases[J]. Nat Rev Drug Discov, 2018,17:588-606.
[10]	Lin Q, Li S, Jiang N, et al. PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation[J]. Redox Biol, 2019,26:101254.doi: 10.1016/j.redox.2019.101254.
[11]	Fu J, Wu H. Structural mechanisms of NLRP3 inflammasome assembly and activation[J]. Annu Rev Immunol, 2023,41:301-316.
[12]	Abais JM, Xia M, Zhang Y, et al. Redox regulation of NLRP3 inflammasomes: ROS as trigger or effector?[J]. Antioxid Redox Signal, 2015,22:1111-1129.
[13]	Mahoney-Sanchez L, Bouchaoui H, Ayton S, et al. Ferroptosis and its potential role in the physiopathology of Parkinson's Disease[J]. Prog Neurobiol, 2021,196:101890.doi: 10.1016/j.pneurobio.2020.101890.
[14]	Han Y, Xu X, Tang C, et al. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis[J]. Redox Biol, 2018,16:32-46.
[15]	Bouyahya A, El MN, Oumeslakht L, et al. Preclinical and clinical antioxidant effects of natural compounds against oxidative stress-induced epigenetic instability in tumor cells[J]. Antioxidants (Basel), 2021,10:1553.doi: 10.3390/antiox10101553.

NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤

NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B

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