NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤

doi:10.16352/j.issn.1001-6325.2025.08.1010

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (8): 1010-1015.doi: 10.16352/j.issn.1001-6325.2025.08.1010

NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤

计泽朝, 张琪, 阿不都许库尔·阿不力米提^*

新疆医科大学基础医学院组织与胚胎学教研室,新疆乌鲁木齐 830000

收稿日期:2024-09-26 修回日期:2024-12-31 出版日期:2025-08-05 发布日期:2025-07-11
通讯作者: ^*806613359@qq.com
基金资助:
国家自然科学基金(32060158)

NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B

JI Zezhao, ZHANG Qi, Abuduxukuer·ABULIMITI^*

Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830000, China

Received:2024-09-26 Revised:2024-12-31 Online:2025-08-05 Published:2025-07-11
Contact: ^*806613359@qq.com

摘要/Abstract

摘要： 目的研究活性氧(ROS)和细胞焦亡在胺碘酮诱导的肺上皮细胞损伤中的作用机制。方法 MTT法检测胺碘酮、胺碘酮+乙酰半胱氨酸(ROS抑制剂,NAC)、胺碘酮+NF-κB抑制剂组细胞存活率,DCFA探针检测活性氧水平,ELISA检测上清液中IL-6、IL-18、IL-1β、TNF-α水平,Western Blot检测NLRP3、Caspase-1 p20、GSDMD-N蛋白水平。结果胺碘酮浓度依赖性降低细胞存活率(P＜0.05),NAC和NF-κB抑制剂减轻胺碘酮的细胞毒性(P＜0.05)。胺碘酮诱导肺上皮细胞中ROS、炎性因子、焦亡蛋白的水平上升(P＜0.05),而NAC和NF-κB抑制剂可以降低这些指标的水平(P＜0.05)。结论 NF-κB抑制剂或NAC减轻胺碘酮体外诱导肺上皮细胞损伤。

关键词: 胺碘酮, 活性氧, 细胞损伤, NF-κB

Abstract: Objective To investigate the mechanism of reactive oxygen species (ROS) and pyroptosis in amiodarone-induced lung epithelial cell injury. Methods The viability of cells treated with amiodarone, amiodarone+N-acetylcysteine(ROS inhibitor,NAC) and amiodarone+NF-κB inhibitors was detected by MTT assay. ROS level was detected by DCFA probe and the levels of IL-6, IL-18, IL-1β and TNF-α in the supernatant were detected by ELISA; NLRP3. Caspase-1 p20 and GSDMD-N protein were detected by Western blot. Results Amiodarone concentration-dependently reduced cell viability(P＜0.05), NAC and NF-κB attenuated the cytotoxicity of amiodarone(P＜0.05). Amiodarone increased levels of reactive oxygen species, inflammatory factors and pyroptosis-related proteins in lung epithelial cells(P＜0.05), while NAC and NF-κB inhibitors reduced the levels of these indicators(P＜0.05). Conclusions NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line in vitro.

Key words: amiodarone, reactive oxygen species, cell injury, NF-κB

中图分类号:

计泽朝, 张琪, 阿不都许库尔·阿不力米提. NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤[J]. 基础医学与临床, 2025, 45(8): 1010-1015.

JI Zezhao, ZHANG Qi, Abuduxukuer·ABULIMITI. NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B[J]. Basic & Clinical Medicine, 2025, 45(8): 1010-1015.

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NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤

NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B

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