滨蒿内酯抑制结肠癌细胞系HCT116的增殖和侵袭

doi:10.16352/j.issn.1001-6325.2025.11.1429

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (11): 1429-1437.doi: 10.16352/j.issn.1001-6325.2025.11.1429

滨蒿内酯抑制结肠癌细胞系HCT116的增殖和侵袭

韩伟¹, 潘伟¹, 张曼¹, 高翔宇¹, 康心凯¹, 朱志博¹, 刘瑞廷^2*

陕西省人民医院 1.医学装备部; 2.普外一科,陕西西安 710068

收稿日期:2024-11-27 修回日期:2025-02-26 出版日期:2025-11-05 发布日期:2025-10-24
通讯作者: ^*fgpeter82Dr@163.com
基金资助:
陕西省技术创新引导专项基金(2022QFY01-08)

Scoparone inhibits proliferation and invasion of colon cancer cell line HCT116

HAN Wei¹, PAN Wei¹, ZHANG Man¹, GAO Xiangyu¹, KANG Xinkai¹, ZHU Zhibo¹, LIU Ruiting^2*

1. Department of Medical Equipment; 2. Department of General Surgery 1, Shaanxi Provincial People's Hospital, Xi'an 710068, China

Received:2024-11-27 Revised:2025-02-26 Online:2025-11-05 Published:2025-10-24
Contact: ^*fgpeter82Dr@163.com

摘要/Abstract

摘要： 目的探究滨蒿内酯(Sco)对结肠癌细胞系HCT116增殖和侵袭,及对表皮生长因子受体(EGFR)表达的影响。方法 1)将HCT116细胞分为对照组、50Sco组、100Sco组和200Sco组。对照组细胞用正常培养基培养48 h。50Sco、100Sco和200Sco组细胞分别加入50、100和200 μmol/L滨蒿内酯培养48 h。2)在过表达EGFR实验中,将HCT116细胞分为对照组、NC-200Sco组、NC-LV+200Sco组和EGFR-LV+200Sco组;对照组细胞采用DMEM培养基培养48 h,NC-200Sco组细胞加入200 μmol/L滨蒿内酯培养48 h;NC-LV+200Sco组和EGFR-LV+200Sco组先将NC-LV和EGFR-LV感染至HCT116细胞,然后采用200 μmol/L滨蒿内酯培养48 h。采用MTT法和EdU染色检测细胞增殖;采用流式细胞测量术检测细胞凋亡;采用Transwell小室法检测细胞侵袭。采用RT-qPCR检测EGFR mRNA水平;采用Western blot检测EGFR、Bcl-2、Bax、基质金属蛋白酶(MMP)-2、MMP-9蛋白水平。结果与对照组比较,50Sco组、100Sco组和200Sco组的相对细胞活力、EdU阳性细胞比例和侵袭细胞数量均降低(P＜0.05),细胞凋亡率和Bax蛋白表达水平升高(P＜0.05),Bcl-2、MMP-2和MMP-9蛋白表达水平降低(P＜0.05),EGFR的mRNA和蛋白表达水平降低(P＜0.05)。与NC-200Sco组和NC-LV+200Sco组比较,EGFR-LV+200Sco组的EGFR的mRNA和蛋白表达水平升高(P＜0.05),相对细胞活力、EdU阳性细胞比例和侵袭细胞数量均升高(P＜0.05),细胞凋亡率和Bax蛋白表达水平降低(P＜0.05),Bcl-2、MMP-2和MMP-9蛋白表达水平升高(P＜0.05)。结论滨蒿内酯具有体外抗结肠癌细胞活性,并可能通过抑制EGFR抑制结肠癌细胞的增殖和侵袭。

关键词: 结肠癌, 滨蒿内酯, 表皮生长因子受体, 增殖, 侵袭

Abstract: Objective To investigate the effects of scoparone (Sco) on proliferation and invasion of colon cancer cell line HCT116, and its effect on the expression of epidermal growth factor receptor (EGFR). Methods 1)HCT116 cells were divided into control group, 50Sco group, 100Sco group and 200Sco group. The cells in control group were incubated with culture medium for 48 hrs. The 50Sco group, 100Sco group and 200Sco group were incubated with 50, 100 and 200 μmol/L scoparone for 48 hrs respectively. 2)HCT116 cells were divided into control group, NC-200Sco group, NC-LV+200Sco group and EGFR-LV+200Sco group. The control group was incubated with normal culture medium for 48 hrs. NC-200Sco group was incubated with 200 μmol/L scoparone for 48 hrs. NC-LV and EGFR-LV were infected into HCT116 cells in NC-LV+200Sco group and EGFR-LV+200Sco group, then incubated with 200 μmol/L scoparone for 48 hrs. Cell proliferation was detected by MTT assay and EdU staining, cell apoptosis was detected by flow cytometry and cell invasion was detected by Transwell assay. EGFR mRNA was detected by RT-qPCR, the level of EGFR, Bcl-2, Bax, matrix metalloproteinase (MMP)-2 and MMP-9 protein was detected by Western blot. Results Compared to the control group, the cell viability, proportion of EdU positive cells and counting number of invasive cells in 50Sco group, 100Sco group and 200Sco group all decreased (P＜0.05). Cell apoptosis rate and Bax protein expression increased (P＜0.05), the protein expression of Bcl-2, MMP-2 and MMP-9 decreased (P＜0.05). mRNA and protein expression of EGFR were decreased (P＜0.05). Compared with NC-200 Sco group and NC-LV+200Sco group, the expression level of mRNA and protein of EGFR in EGFR-LV+200Sco group was increased (P＜0.05). Cell viability, proportion of EdU positive cells and counting number of invasive cells all increased (P＜0.05). The cell apoptosis rate and Bax protein expression level were decreased (P＜0.05). The protein expression of Bcl-2, MMP-2 and MMP-9 was increased(P＜0.05). Conclusions Scoparone has anti-colon cancer cell activity and inhibits proliferation as well as invasion of colon cancer cells through inhibition of EGFR.

Key words: colon cancer, scoparone, epidermal growth factor receptor, proliferation, invasion

中图分类号:

R735.3+5

韩伟, 潘伟, 张曼, 高翔宇, 康心凯, 朱志博, 刘瑞廷. 滨蒿内酯抑制结肠癌细胞系HCT116的增殖和侵袭[J]. 基础医学与临床, 2025, 45(11): 1429-1437.

HAN Wei, PAN Wei, ZHANG Man, GAO Xiangyu, KANG Xinkai, ZHU Zhibo, LIU Ruiting. Scoparone inhibits proliferation and invasion of colon cancer cell line HCT116[J]. Basic & Clinical Medicine, 2025, 45(11): 1429-1437.

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滨蒿内酯抑制结肠癌细胞系HCT116的增殖和侵袭

Scoparone inhibits proliferation and invasion of colon cancer cell line HCT116

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