虫草素抑制TNF-α诱导的人角质形成细胞系HaCaT增殖

doi:10.16352/j.issn.1001-6325.2023.09.1390

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (9): 1390-1393.doi: 10.16352/j.issn.1001-6325.2023.09.1390

虫草素抑制TNF-α诱导的人角质形成细胞系HaCaT增殖

梁辉勇^1,2, 韦秋慧², 丁亚¹, 彭小青¹, 姚静³, 苏齐鉴^1,4*

1.广西中医药大学研究生院,广西南宁 530200;
2.前海人寿广西医院皮肤科,广西南宁 530200;
3.广西中医药大学附属瑞康医院中西医结合转化医学中心,广西南宁 530011;
4.钦州市第一人民医院科技科,广西钦州 535099

收稿日期:2022-10-20 修回日期:2023-02-27 出版日期:2023-09-05 发布日期:2023-09-01
通讯作者: ^*agansue@163.com
基金资助:
广西中医药大学广西一流学科建设开放课题(2019XK158)

Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α

LIANG Huiyong^1,2, WEI Qiuhui², DING Ya¹, PENG Xiaoqing¹, YAO Jing³, SU Qijian^1,4*

1. Graduate School, Guangxi University of Chinese Medicine, Nanning 530200;
2. Department of Dermatology,Foresea Life Insurance Guangxi Hospital, Nanning 530200;
3. Centre for Translational Medical Research in Integrative Chinese &Western Medicine, Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011;
4. Department of Science and Technology, the First People's Hospital of Qinzhou, Qinzhou 535099, China

Received:2022-10-20 Revised:2023-02-27 Online:2023-09-05 Published:2023-09-01
Contact: ^*agansue@163.com

摘要/Abstract

摘要： 目的探讨虫草素对肿瘤坏死因子α(TNF-α)诱导人角质形成细胞系HaCaT增殖、凋亡和炎性反应的影响。方法将HaCaT细胞分为对照组(control)、模型组(model)及低、中、高浓度虫草素干预组(25 μmol/L、50 μmol/L、100 μmol/L),先用40 μg/L TNF-α对模型组、虫草素干预组诱导24 h,制作银屑病(PS)样细胞模型;之后虫草素干预组加入相应浓度的虫草素,继续培养24 h;用CCK-8法检测细胞增殖,用流式细胞仪检测细胞凋亡,用RT-qPCR检测炎性细胞因子白介素(IL)-8、IL-1β mRNA相对表达量。结果虫草素干预24 h后细胞增殖率下降(P＜0.05),细胞凋亡率升高(P＜0.05),IL-8和 IL-1β 的mRNA表达水平下降(P＜0.05)。结论虫草素可抑制TNF-α诱导的人角质形成细胞系增殖、炎性反应并促进细胞凋亡。

关键词: 人角质形成细胞, 虫草素, 银屑病, 细胞增殖, 炎性反应

Abstract: Objective To study the effect of cordycepin on proliferation, apoptosis and inflammatory reaction of human keratinocyte cell line HaCaT induced by TNF-α. Methods HaCaT cells were divided into control group, model group and intervention groups including low, middle, high dose (cordycepin 25 μmol/L, 50 μmol/L and 100 μmol/L, respectively). HaCaT cells were incubated with by 40 μg/L TNF-α in model group and cordycepin intervention group for 24 hours. Then, the cordycepin intervention groups were incubated with corresponding cordycepin for 24 hours. Cell proliferation was detected by CCK-8 method. Apoptosis was detected by flow cytometry. And mRNA expression of interleukin (IL)-8, IL-1β was detected by RT-qPCR. Results After 24 hours of cordycepin incubation, cell proliferation rate decreased (P＜0.05), cell apoptosis rate increased(P＜0.05)and mRNA expression of IL-8 and IL-1β decreased (P＜0.05). Conclusions Cordycepin can inhibit TNF-α-induced proliferation and inflammatory reaction of HaCaT cells and promote apoptosis of these cells.

Key words: human keratinocyte, cordycepin, psoriasis, cell proliferation, inflammation

中图分类号:

R758.63
R275

梁辉勇, 韦秋慧, 丁亚, 彭小青, 姚静, 苏齐鉴. 虫草素抑制TNF-α诱导的人角质形成细胞系HaCaT增殖[J]. 基础医学与临床, 2023, 43(9): 1390-1393.

LIANG Huiyong, WEI Qiuhui, DING Ya, PENG Xiaoqing, YAO Jing, SU Qijian. Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α[J]. Basic & Clinical Medicine, 2023, 43(9): 1390-1393.

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虫草素抑制TNF-α诱导的人角质形成细胞系HaCaT增殖

Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α

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