敲除Mcart1加重脓毒症模型小鼠巨噬细胞炎性反应水平

doi:10.16352/j.issn.1001-6325.2025.05.0616

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (5): 616-621.doi: 10.16352/j.issn.1001-6325.2025.05.0616

敲除Mcart1加重脓毒症模型小鼠巨噬细胞炎性反应水平

刘宇涵, 王莹莹, 王钰铖, 郭磊, 鞠瑞^*

中国医学科学院基础医学研究所北京协和医学院基础学院药理系,北京 100005

收稿日期:2025-01-02 修回日期:2025-02-20 出版日期:2025-05-05 发布日期:2025-04-23
通讯作者: ^* jurui1984@163.com
基金资助:
呼吸和共病全国重点实验室开放课题基金(2060204);科技创新2030 -“脑科学与类脑研究”重大项目 2021年度定向委托项目(2021ZD0201100); “人脑组织资源库及地区脑库协作网络平台”课题1(2021ZD0201101)

Mcart1 knockout enhances the level of macrophage inflammatory response in sepsis model mice

LIU Yuhan, WANG Yingying, WANG Yucheng, GUO Lei, JU Rui^*

Department of Pharmacology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China

Received:2025-01-02 Revised:2025-02-20 Online:2025-05-05 Published:2025-04-23
Contact: ^* jurui1984@163.com

摘要/Abstract

摘要： 目的通过体内外模型检测Mcart1敲除对巨噬细胞急性炎性反应的影响。方法使用脂多糖(LPS),在Mcart1敲除的小鼠上构建小鼠脓毒症模型并检测生存期LPS致炎小鼠骨髓来源的巨噬细胞(BMDM)培养于DMEM高糖培养基中,采用RT-qPCR检测BMDM在LPS刺激后M1和M2相关细胞因子的mRNA水平;采用ELISA检测脓毒症小鼠血清中炎性反应相关介质的表达水平。结果相比野生型小鼠(Mcart1^flox/flox),Mcart1敲除小鼠(Mcart1^Lyz2-Cre)的脓毒症生存期显著缩短(P＜0.001),Mcart1^Lyz2-Cre鼠的BMDM细胞在LPS致炎后M1相关介质的mRNA水平上调(P＜0.05),M2相关介质的mRNA水平下调(P＜0.05),脓毒症Mcart1^Lyz2-Cre小鼠血清中M1相关介质表达上调(P＜0.01)。结论 Mcart1敲除可显著升高巨噬细胞的炎性反应水平,并加重小鼠的病理症状。

关键词: 巨噬细胞, 脓毒症, Mcart1, 炎性反应

Abstract: Objective To investigate the effect of Mcart1 knockout on acute inflammation of macrophages in vitro and in vivo. Methods The Mcart1 knockout mice were used to establish a sepsis model induced by lipopolysaccharide(LPS), and the survival period was measured. Bone marrow derived macrophages(BMDM) of LPS-induced inflammation mice were cultured in DMEM high-glucose medium. The mRNA levels of M1 and M2 related cytokines of BMDM after LPS stimulation were detected by RT-qPCR. The expression level of inflammation-related cytokines in serum of sepsis mice was detected by ELISA. Results Compared with wild type mice(Mcart1^flox/flox), the survival time length of sepsis in Mcart1 knockout mice(Mcart1^Lyz2-Cre)was significantly shortened(P＜0.001). After inflammation, the mRNA level of M1-related cytokines was up-regulated in BMDM cells of Mcart1^Lyz2-Cre mice(P＜0.05); The mRNA level of M1-related cytokines was down-regulated(P＜0.05); The expression of M1-related mediators in serum of sepsis Mcart1^Lyz2-Cre mice was up-regulated(P＜0.01). Conclusions Mcart1 knockout can significantly raise the inflammatory response of macrophages and aggravate the pathological symptoms of sepsis mice.

Key words: macrophages, sepsis, Mcart1, inflammation

中图分类号:

R392.12

刘宇涵, 王莹莹, 王钰铖, 郭磊, 鞠瑞. 敲除Mcart1加重脓毒症模型小鼠巨噬细胞炎性反应水平[J]. 基础医学与临床, 2025, 45(5): 616-621.

LIU Yuhan, WANG Yingying, WANG Yucheng, GUO Lei, JU Rui. Mcart1 knockout enhances the level of macrophage inflammatory response in sepsis model mice[J]. Basic & Clinical Medicine, 2025, 45(5): 616-621.

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敲除Mcart1加重脓毒症模型小鼠巨噬细胞炎性反应水平

Mcart1 knockout enhances the level of macrophage inflammatory response in sepsis model mice

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