FABP4表达上调促进人子宫内膜癌细胞系HEC-1-A增殖和迁移

doi:10.16352/j.issn.1001-6325.2022.09.1356

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (9): 1356-1361.doi: 10.16352/j.issn.1001-6325.2022.09.1356

FABP4表达上调促进人子宫内膜癌细胞系HEC-1-A增殖和迁移

贺晶¹, 李艳¹, 王延峰², 鲍慧^3*

1. 延安大学附属医院妇科, 陕西延安 716000;
2. 延安大学附属医院检验科, 陕西延安 716000;
3. 延安大学附属医院肿瘤科, 陕西延安 716000

收稿日期:2021-05-17 修回日期:2021-12-13 出版日期:2022-09-05 发布日期:2022-09-02
通讯作者: 13209111816@163. com
基金资助:
陕西省教育厅科研计划项目(15JK1838)

Up-expression of FABP4 promotes the proliferation and migration of endometrial cancer cell line HEC-1-A

HE Jing¹, LI Yan¹, WANG Yan-feng², BAO Hui^3*

1. Department of Gynaecology, Affiliated Hospital of Yan'an University,Yan'an 716000, China;
2. Department of Clinical Laboratory, Affiliated Hospital of Yan'an University,Yan'an 716000, China;
3. Department of Oncology, Affiliated Hospital of Yan'an University,Yan'an 716000, China

Received:2021-05-17 Revised:2021-12-13 Online:2022-09-05 Published:2022-09-02

摘要/Abstract

摘要： 目的探讨脂肪酸结合蛋白4(FABP4)在子宫内膜癌中的表达及其对子宫内膜癌细胞系HEC-1-A增殖、迁移及侵袭的影响。方法 RT-qPCR检测子宫内膜癌组织及癌旁组织FABP4 mRNA的表达水平,分析FABP4表达与子宫内膜癌临床病理特征的相关性。将HEC-1-A细胞分为对照组、FABP4阴性对照(si-NC)组和干扰FABP4(si-FABP4)组。RT-qPCR检测HEC-1-A细胞FABP4 mRNA的表达;MTT法检测HEC-1-A细胞增殖;Transwell小室法检测细胞迁移与侵袭;免疫印迹法检测HEC-1-A细胞FABP4、基质金属蛋白酶(MMP)-2和MMP-9蛋白的表达。结果子宫内膜癌组织中FABP4 mRNA表达水平显著高于癌旁组织(P＜0.05);FABP4表达与肿瘤TNM分期和淋巴结转移有关(P＜0.05);干扰FABP4表达可显著抑制子宫内膜癌细胞增殖、迁移与侵袭能力。结论 FABP4在子宫内膜癌中表达上调,干扰FABP4表达可抑制子宫内膜癌细胞增殖、迁移和侵袭。

关键词: 脂肪酸结合蛋白4, 子宫内膜癌, 增殖, 迁移, 侵袭

Abstract: Objective To investigate the expression of fatty acid binding protein 4 (FABP4) in endometrial cancer and its effects on the proliferation, migration and invasion of endometrial cancer cells line HEC-1-A. Methods The expression level of FABP4 mRNA in endometrial cancer tissues and adjacent normal tissues was detected with RT-qPCR. The correlation between FABP4 mRNA expression and pathological changes of endometrial cancer was analyzed. HEC-1-A cells were cultured in vitro and were randomly divided into control group, FABP4 negative control (si-NC) group and interference FABP4 (si-FABP4) group. RT-qPCR, MTT and Transwell assays were used respectively to detect the FABP4 mRNA expression in HEC-1-A cells, the proliferation, migration and invasion of HEC-1-A cells. Western blot was used to detect the protein expression of FABP4, matrix metalloproteinase (MMP)-2 and MMP-9 of HEC-1-A cells in each group. Results The expression level of FABP4 mRNA in endometrial cancer tissues was significantly higher than that in tissues adjacent to cancer (P＜0.05); The FABP4 mRNA expression was related to tumor TNM staging and lymph node metastasis (P＜0.05).Interference with FABP4 expression could significantly inhibit the proliferation, migration and invasion of endometrial cancer cells. Conclusions Expression of FABP4 is up-regulated in endometrial cancer and interference with FABP4 expression can inhibit the proliferation, migration and invasion of endometrial cancer cells.

Key words: fatty acid binding protein 4, endometrial carcinoma, proliferation, migration, invasion

中图分类号:

R737.33

贺晶, 李艳, 王延峰, 鲍慧. FABP4表达上调促进人子宫内膜癌细胞系HEC-1-A增殖和迁移[J]. 基础医学与临床, 2022, 42(9): 1356-1361.

HE Jing, LI Yan, WANG Yan-feng, BAO Hui. Up-expression of FABP4 promotes the proliferation and migration of endometrial cancer cell line HEC-1-A[J]. Basic & Clinical Medicine, 2022, 42(9): 1356-1361.

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FABP4表达上调促进人子宫内膜癌细胞系HEC-1-A增殖和迁移

Up-expression of FABP4 promotes the proliferation and migration of endometrial cancer cell line HEC-1-A

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