Circ_0032821参与索拉非尼抑制乳腺癌细胞系T47D增殖、迁移和侵袭

doi:10.16352/j.issn.1001-6325.2022.09.1350

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (9): 1350-1354.doi: 10.16352/j.issn.1001-6325.2022.09.1350

Circ_0032821参与索拉非尼抑制乳腺癌细胞系T47D增殖、迁移和侵袭

张晓雷^1*, 刘静², 李朝萍³

1. 黄河三门峡医院乳腺外科, 河南三门峡 472000;
2. 黄河三门峡医院健康体检科, 河南三门峡 472000;
3. 郑州大学附属洛阳中心医院肿瘤中心, 河南洛阳 471000

收稿日期:2021-06-11 修回日期:2021-12-13 出版日期:2022-09-05 发布日期:2022-09-02
通讯作者: zhangxl202106@126.com
基金资助:
河南省科技发展计划(1603002A-1)

Circ_0032821 is involved in sorafenib's inhibition of the proliferation, migration and invasion of breast cancer cell line T47D

ZHANG Xiao-lei^1*, LIU Jing², LI Chao-ping³

1. Department of Breast Surgery, Sanmenxia Hospital of Yellow River, Sanmenxia 472000;
2. Department of Health Examination, Sanmenxia Hospital of Yellow River, Sanmenxia 472000;
3. Department of Cancer Center, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471000, China

Received:2021-06-11 Revised:2021-12-13 Online:2022-09-05 Published:2022-09-02

摘要/Abstract

摘要： 目的探讨索拉非尼对乳腺癌细胞系T47D增殖、迁移、侵袭的影响和可能的作用机制。方法通过CCK-8法、平板集落实验、划痕愈合实验和Transwell小室法检测索拉非尼(2.5、5和10 μmol/L)对T47D细胞增殖、集落形成、迁移和侵袭的影响。RT-qPCR检测circ_0032821表达。将circ_0032821小干扰RNA(si-circ_0032821)、circ_0032821过表达质粒分别转染T47D细胞,检测干扰circ_0032821或过表达circ_0032821联合索拉非尼对T47D细胞恶性行为的影响。结果索拉非尼处理后,T47D细胞增殖抑制率显著升高(P＜0.05),集落形成数、划痕愈合率、侵袭数以及circ_0032821表达显著降低(P＜0.05)。沉默circ_0032821后,T47D细胞增殖抑制率显著升高(P＜0.05),集落形成数、划痕愈合率、侵袭数显著降低(P＜0.05)。过表达circ_0032821明显减弱索拉非尼对T47D细胞增殖、迁移、集落形成和侵袭的影响。结论索拉非尼可抑制T47D细胞增殖、迁移和侵袭,其机制可能与下调circ_0032821表达有关。

关键词: 索拉非尼, 乳腺癌, 增殖, 迁移, circ_0032821

Abstract: Objective To investigate the effect and possible mechanism of sorafenib on the proliferation, migration and invasion of breast cancer cell line T47D. Methods Effects of sorafenib (2.5, 5 and 10 μmol/L) on T47D cells proliferation, colony formation, migration and invasion were detected by CCK-8 experiment, plate cloning experiment, scratch healing experiment and Transwell experiment. RT-qPCR was applied to detect circ_0032821 expression. The circ_0032821 small interfering RNA(si-circ_0032821) and circ_0032821 over-expression plasmid were transfected into T47D cells respectively. And the effect of interference with circ_0032821 or circ_0032821 over-expression combined with sorafenib on the malignant behavior of T47D cells was detected. Results The cell proliferation inhibition rate T47D cells were significantly increased after sorafenib treatment (P＜0.05); The colony formation number, scratch healing rate, invasion number, and circ_0032821 expression were significantly decreased(P＜0.05).After silencing with the expression of circ_0032821, the proliferation inhibition rate of T47D cells was significantly increased (P＜0.05); The colony formation number, scratch healing rate and invasion number were significantly reduced(P＜0.05). Circ_0032821 over-expression significantly reduced the effects of sorafenib on the proliferation, migration colony formation and invasion of T47D cells (P＜0.05). Conclusions Sorafenib can inhibit T47D cell proliferation, migration and invasion. And the mechanism may be explained by the down-regulation of circ_0032821.

Key words: sorafenib, breast cancer, proliferation, migration, circ_0032821

中图分类号:

R737.9

张晓雷, 刘静, 李朝萍. Circ_0032821参与索拉非尼抑制乳腺癌细胞系T47D增殖、迁移和侵袭[J]. 基础医学与临床, 2022, 42(9): 1350-1354.

ZHANG Xiao-lei, LIU Jing, LI Chao-ping. Circ_0032821 is involved in sorafenib's inhibition of the proliferation, migration and invasion of breast cancer cell line T47D[J]. Basic & Clinical Medicine, 2022, 42(9): 1350-1354.

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Circ_0032821参与索拉非尼抑制乳腺癌细胞系T47D增殖、迁移和侵袭

Circ_0032821 is involved in sorafenib's inhibition of the proliferation, migration and invasion of breast cancer cell line T47D

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