PTOV1表达水平对结直肠癌患者预后的影响

doi:10.16352/j.issn.1001-6325.2022.08.1176

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (8): 1176-1181.doi: 10.16352/j.issn.1001-6325.2022.08.1176

PTOV1表达水平对结直肠癌患者预后的影响

谢思安¹, 陈奕阳², 徐俊玄¹, 宁婷婷¹, 张楠¹, 朱圣韬¹, 张澍田^1*

首都医科大学北京友谊医院 1.消化内科; 2.检验科,北京 100050

收稿日期:2021-09-05 修回日期:2022-06-26 出版日期:2022-08-05 发布日期:2022-08-01
通讯作者: ^*zhangshutian@ccmu.edu.cn
基金资助:
国家自然科学基金(82103394);首都医科大学附属北京友谊医院“友谊种子计划”人才项目(YYZZ202028)

Effect of PTOV1 expression on prognosis of colorectal cancer patients

XIE Si-an¹, CHEN Yi-yang², XU Jun-xuan¹, NING Ting-ting¹, ZHANG Nan¹, ZHU Sheng-tao¹, ZHANG Shu-tian^1*

1. Department of Gastroenterology; 2. Department of Laboratory Medicine, Beijing Friendship Hospital,Capital Medical University, Beijing 100050,China

Received:2021-09-05 Revised:2022-06-26 Online:2022-08-05 Published:2022-08-01
Contact: ^*zhangshutian@ccmu.edu.cn

摘要/Abstract

摘要： 目的基于生物信息学分析前列腺肿瘤过表达1 (PTOV1)在结直肠癌 (CRC)中的表达对CRC患者预后的影响,初步验证并探讨其对CRC细胞增殖的影响。方法结合生物信息学和生物学实验验证分析CRC组织及细胞系中PTOV1的表达情况;蛋白免疫印迹和RT-qPCR验证PTOV1表达变化;在SW480细胞系中敲低PTOV1后通过集落形成实验和EdU实验探讨PTOV1对CRC细胞增殖的影响。结果 PTOV1在CRC组织和细胞系中表达显著上调(P＜0.05);进一步通过生信学分析表明,PTOV1作为一个癌基因与CRC不良预后相关;体外实验表明敲低PTOV1表达抑制SW480细胞增殖(P＜0.05)。结论 PTOV1在CRC中高表达且与CRC患者不良预后相关,是CRC进展的潜在预后生物标志物。

关键词: 结直肠癌, PTOV1, 预后, 生物标志物, 增殖

Abstract: Objective To analyze the effect of over-expression of prostate tumor 1 (PTOV1) on the prognosis of colorectal cancer (CRC) based on bioinformatics, and to demonstrate the effect of PTOV1 on the proliferation of CRC cells. Methods Combined with bioinformatics and biological experiments, PTOV1 expression in CRC tissues and cell lines was analyzed. PTOV1 expression was confirmed by Western blot and RT-qPCR. The effect of PTOV1 on the proliferation of CRC cells was investigated by colony formation assay and EdU assay after transfection by PTOV1 siRNA in SW480 cell line. Results PTOV1 expression was significantly up-regulated in CRC tissues and cell lines (P＜0.05). More biological experiments results showed that PTOV1 as an oncogene was associated with poor prognosis of CRC. In vitro experiments showed that PTOV1 knockdown inhibited the proliferation of SW480 cells(P＜0.05). Conclusions PTOV1 is highly expressed in CRC and is associated with poor prognosis in CRC patients. PTOV1 is a potential prognostic biomarker for the progression of CRC.

Key words: colorectal cancer, PTOV1, prognosis, biomarkers, proliferation

中图分类号:

R735.7

谢思安, 陈奕阳, 徐俊玄, 宁婷婷, 张楠, 朱圣韬, 张澍田. PTOV1表达水平对结直肠癌患者预后的影响[J]. 基础医学与临床, 2022, 42(8): 1176-1181.

XIE Si-an, CHEN Yi-yang, XU Jun-xuan, NING Ting-ting, ZHANG Nan, ZHU Sheng-tao, ZHANG Shu-tian. Effect of PTOV1 expression on prognosis of colorectal cancer patients[J]. Basic & Clinical Medicine, 2022, 42(8): 1176-1181.

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PTOV1表达水平对结直肠癌患者预后的影响

Effect of PTOV1 expression on prognosis of colorectal cancer patients

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