葛根素减轻LPS诱导的小鼠肾脏炎性反应

doi:10.16352/j.issn.1001-6325.2022.11.1727

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (11): 1727-1730.doi: 10.16352/j.issn.1001-6325.2022.11.1727

葛根素减轻LPS诱导的小鼠肾脏炎性反应

冯娜¹, 唐杰¹, 贺细菊¹, 田宗滢¹, 史丹丹¹, 余晶晶^2*

1.湖北医药学院基础医学院人体解剖学教研室,湖北十堰 442000;
2.湖北医药学院附属人民医院, 湖北十堰 442000

收稿日期:2021-07-31 修回日期:2022-04-15 出版日期:2022-11-05 发布日期:2022-11-01
通讯作者: ^* 25674813@qq.com
基金资助:
湖北省卫生计生委科研项目(WJ2017 M213)

Puerarin attenuates LPS-induced renal inflammatory response of mice

FENG Na¹, TANG Jie¹, HE Xi-ju¹, TIAN Zong-ying¹, SHI Dan-dan¹, YU Jing-jing^2*

1. Department of Anatomy, Basic Medical College, Hubei University of Medicine, Shiyan 442000;
2. Renmin Hospital, Hubei University of Medicine, Shiyan 442000, CHina

Received:2021-07-31 Revised:2022-04-15 Online:2022-11-05 Published:2022-11-01
Contact: ^* 25674813@qq.com

摘要/Abstract

摘要： 目的探讨葛根素(PUE)是否减轻脂多糖(LPS)诱导的小鼠炎性反应。方法选择30名健康体检者为对照(control)组,45例严重感染(SI)非肾损伤患者作为N-SI组,30例严重感染致肾损伤患者作SI组,收集患者外周血样本。将50只小鼠随机分为对照(Ctrl)组,LPS组,LPS+PUE(10、25、50 mg/kg·d, ip)组,PUE在给予LPS后1 h应用,24 h后处死小鼠,采集血液。ELISA检测血清Scr、BUN、IL-6和IL-33水平。结果与对照组和N-SI组相比,SI组患者血清中BUN、Scr、IL-6和 IL-33水平显著升高(P＜0.05)。小鼠LPS组与对照组相比,血清中肾功能指标BUN和Scr均显著增高 (P＜0.05);与LPS组相比,PUE可显著降低BUN、Scr、MDA、IL-33 和IL-6的表达水平,并使SOD的活性升高(P＜0.05)。结论 PUE可减轻LPS诱导的小鼠炎性反应。

关键词: 葛根素, 脂多糖, 炎性反应

Abstract: Objective To investigate whether puerarin (PUE) can alleviate lipopolysarccharide(LPS)-induced renal inflammatory response in mice. Methods Thirty health control, 45 severe infection with no renal injury(N-SI group), 30 severe infection with renal injury(SI group) were enrolled in this study, Serum samples were collected. 50 mice were randomly divided into 5 groups, including control(Ctrl) group, LPS group, and LPS+PUE(10, 25, 50 mg/kg·d, IP) groups. PUE was applied 1 h after LPS administration. And mice were sacrificed after 24 h and blood sample was collected. MDA, SOD, IL-33 and IL-6 expression were detected by ELISA. Results BUN, Scr, IL-6 and IL-33 in SI group were significantly increased compared with control group and N-SI group (P＜0.05). BUN and Scr of LPS group were significantly increased compared with control group. PUE decreased BUN, Scr, MDA, IL-33 and IL-6 expression and increased activity of SOD as compared with LPS group(P＜0.05). Conclusions PUE can alleviate LPS-induced renal inflammatory response in mice.

Key words: puerarin, lipopolysarccharide(LPS), inflammation response

中图分类号:

R692

冯娜, 唐杰, 贺细菊, 田宗滢, 史丹丹, 余晶晶. 葛根素减轻LPS诱导的小鼠肾脏炎性反应[J]. 基础医学与临床, 2022, 42(11): 1727-1730.

FENG Na, TANG Jie, HE Xi-ju, TIAN Zong-ying, SHI Dan-dan, YU Jing-jing. Puerarin attenuates LPS-induced renal inflammatory response of mice[J]. Basic & Clinical Medicine, 2022, 42(11): 1727-1730.

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葛根素减轻LPS诱导的小鼠肾脏炎性反应

Puerarin attenuates LPS-induced renal inflammatory response of mice

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