关键词: 寻常型银屑病, 脂肪间充质干细胞, 增殖, 成脂、成骨能力, 免疫调控

Abstract: Objective To study the differences of the biological characteristics and immune regulation function of adipose mesenchymal stem cells (AMSCs)from psoriasis patients and healthy people. Methods AMSCs were isolated and cultured from human psoriatic and healthy adipose tissue, the phenotypes and cell cycle of AMSCs taken from three generation were detected by flow eytometry. Alkaline phosphate enzyme staining and oil red o staining were used respectively to identify their adipogenic and osteogenic capacity . Next，the levels of inflammation antimicrobial proinflammatory factor were detected by PCR and ELISA.Then gene expression profile of AMSCs were screen by gene expression profile chip，as so to bolting the the gene array related with immunology gene. Results We found that there were no significant change in cell morphology, and cell surface markers were expressed high for CD29、CD44、CD73, while lower for CD31、CD45 and HLA-DR. AMSCs of psoriasis patients and healthy people both had the ability of adipogenic and osteogenic differentiation. But the cell cycle showed the third generation AMSCs proliferation rates were slower than of normal control , as compared with healthy controls，adipogenic differentiation ability was stronger. What’more, the levels of inflammatory cytokines in psoriasis group was lower than that in controls such asIL-10、IDO、TGF-β, on the contrary the levels of proinflammatory factor in psoriasis group were higher than that in controls, such as TNF-α，IFN-Y. In addition, gene chip results suggested that psoriasis group AMSCs had obvious expression differences on JAK - STAT pathway with healthy controls. Conclusion Compared with the control, there are significant differences in patients AMSCs proliferation and adipogenic differentiation ability, immune inflammation suppression control ability is weaken,we speculate that this phenomenon may be associated with JAK - STAT immune pathways related to downgrade.

Key words: psoriasis vulgaris, adipose mesenchymal stem cells, proliferation , adipogenic and osteogenic capacity , immune regulation function