关键词: 雄性抑郁大鼠, 生殖, 微波硫灯, 睾酮

Abstract: Objective To investigate the effects of different lighting(sulfur lamp, heat radiation lamp, fluorescent lamp and LED lamp) on reproductive system in depressive male rats. Methods CUMS depressive model of rats in vivo was established through the stimulations of chronic unpredictable mild stress(CUMS), then they were randomly divided into 5 groups：depressive model group, sulfur lamp group, heat radiation lamp group, fluorescent lamp group and LED lamp group. After 45 d of continuous illumination, serum was collected and reproductive organs were removed from rats, then the organ index of testicle，epididymis，seminal vesicle and kidney was calculated, the concentrations of testosterone (T), estradiol (E2), prolactin (PRL) and progesterone (PROG)in the serum were detected by ELISA, the histomorphological lesion of testicle was observed under microscope with hematoxylin-eosin (HE) staining , the expression of melatonin receptors (Mel 1a) , 3β-HSD and P450scc of testicle were detected by Western blot. Results The organ index of testicle，epididymis and seminal vesicle, the concentration of T, E2, PRL and PROG, and the expression of Mel 1a, 3β-HSD and P450scc in the depressive rats were significantly lower than that of the controls(P<0.05), and the seminiferous tubules of the testis of depressive rats were atrophied. However, the level of T, E2, PRL and PROG were increased，the cell morphology of the testicle was improved, and the expression of Mel 1a, 3β-HSD and and P450scc were upregulated after the sulfur lamp lighting in the depressive male rats compared with depressive model group (P<0.05). Conclusions The testis structure and functions of depressive male rats are improved by sulfur lamp lighting.

Key words: depressive male rats, reproduction, sulfur lamp, testosterone