枸橼酸他莫昔芬促进TGF-β1对Leydig细胞系R2C的增殖

doi:10.16352/j.issn.1001-6325.2026.01.0005

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (1): 5-10.doi: 10.16352/j.issn.1001-6325.2026.01.0005

枸橼酸他莫昔芬促进TGF-β1对Leydig细胞系R2C的增殖

王叶庭¹, 吴鹏², 边艳超², 温建勋², 牛丽丽^2*

1.内蒙古妇幼保健院生殖医学男科, 内蒙古呼和浩特 010000;
2.内蒙古医科大学基础医学院,内蒙古呼和浩特 010000

收稿日期:2025-02-18 修回日期:2025-04-29 出版日期:2026-01-05 发布日期:2025-12-29
通讯作者: ^*niulili003@163.com
基金资助:
国家自然科学基金(31760304);内蒙古医科大学重点实验室开放基金(NYFZBL202104)

Tamoxifen citrate promotes proliferation of Leydig cell line R2C after treatment with TGF-β1

WANG Yeting¹, WU Peng², BIAN Yanchao², WEN Jianxun², NIU Lili^2*

1. Department of Reproductive Medicine and Andrology, Inner Mongolia Maternal and Child Health Hospital, Hohhot 010000;
2. School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010000, China

Received:2025-02-18 Revised:2025-04-29 Online:2026-01-05 Published:2025-12-29
Contact: ^*niulili003@163.com

摘要/Abstract

摘要： 目的探讨枸橼酸他莫昔芬(TCT)对TGF-β1作用后的大鼠睾丸间质细胞(Leydig细胞)系R2C的影响。方法以R2C细胞为研究对象。采用CCK-8法检测不同浓度梯度的TCT对正常R2C细胞的增殖活性影响筛选出增殖明显的浓度。正常R2C细胞中加入TGF-β1造模,然后给予显著促进R2C细胞增殖浓度的TCT,检测R2C细胞增殖活性、基础睾酮、雌二醇以及细胞周期的变化。结果 TGF-β1干扰R2C 细胞后,4 ng/mL、5 ng/mL、6 ng/mL TCT处理 24、48、72 h,均可显著促进R2C细胞增殖(P＜0.05);ELISA结果显示, 5 ng/mL、6 ng/mL TCT处理48 h,基础雌二醇水平降低(P＜0.05),基础睾酮水平增高(P＜0.05)。流式细胞测量术结果显示,6 ng/mL药物处理组G1期细胞明显增多,细胞周期可能阻滞在G1期。结论一定剂量的TCT可改善Leyding细胞系R2C因外源性损伤所导致的负面激素水平变化,并促进其增殖。

关键词: 枸橼酸他莫西芬, TGF-β1, Leydig细胞, 雌二醇, 睾酮

Abstract: Objective To investigate the effect of tamoxifen citrate (TCT) on Leydig cell line R2C treated by TGF-β1. Methods R2C cells were used as the research subjects. CCK-8 test was used to detect the effect of different concentration gradients of TCT on the proliferation activity of normal R2C cells to fix on the most effective concentration. TGF-β1 was incubated with R2C cells for modeling, and then TCT was added into the medium at a concentration that significantly promoted R2C cell proliferation. R2C cell proliferation activity, basal testosterone, estradiol and cell cycle were detected. Results TGF-β1 treatment with 4 ng/mL, 5 ng/mL and 6 ng/mL TCT for 24, 48, and 72 hours significantly promoted R2C cell proliferation (P＜0.05); ELISA showed that treatment with 5 ng/mL and 6 ng/mL TCT for 48 hours resulted in a decrease in basal level of estradiol(E2)(P＜0.05) and an increase in basal level of testosterone(T) (P＜0.05). Flow cytometry showed a significant increase cell counting in G1 phase in the 6 ng/mL TCT treatment group, and the cell cycle arrested in the G1 phase. Conclusions A certain dose of TCT can improve the negative hormone level changes caused by exogenous injury of Leydig cell line R2C and promote its proliferation.

Key words: tamoxifen citrate, TGF-β1, Leydig cell, estradiol, testosterone

中图分类号:

R339.21

王叶庭, 吴鹏, 边艳超, 温建勋, 牛丽丽. 枸橼酸他莫昔芬促进TGF-β1对Leydig细胞系R2C的增殖[J]. 基础医学与临床, 2026, 46(1): 5-10.

WANG Yeting, WU Peng, BIAN Yanchao, WEN Jianxun, NIU Lili. Tamoxifen citrate promotes proliferation of Leydig cell line R2C after treatment with TGF-β1[J]. Basic & Clinical Medicine, 2026, 46(1): 5-10.

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枸橼酸他莫昔芬促进TGF-β1对Leydig细胞系R2C的增殖

Tamoxifen citrate promotes proliferation of Leydig cell line R2C after treatment with TGF-β1

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