关键词: 前列腺癌, miR-29c, 血管内皮生长因子, 血管内皮生长因子受体

Abstract: Objective To study the effect of miR-29c on the apoptosis of prostate cancer cell line PC3. Methods The expression of miR-29c, VEGF and VEGFR2 in RWPE-1 and PC3 were detected to verify the different between normal and Cancer cell. The location of p-VEGFR2 was measured by immunofluorescence (IF). After PC3 cell were transfected by miR-29c overexpression adenovirus , the expression level of miR-29c, VEGF, t-VEGFR2, p-VEGFR2, BAX and Bcl-2 were measured by RT-PCR and Western blot , the cell apoptosis rate was detected by the kits of hoechst 33258 and FCM. Results The expression level of miR-29c was lower and VEGF, VEGFR2 was higher in PC3 cell compared with that in RWPE-1(p<0.001). The expression level of miR-29c and BAX were higher and VEGF, P-VEGFR2 and Bcl-2 were lower in Ad-miR-29c group compared with that in control group(p<0.001). Higher apoptosis rate was detected in Ad-miR-29c group compared with control group(p<0.001). Conclusions miR-29c can promotes apoptosis in prostate cancer cell PC3 by inhibiting VEGF/VEGFR2 signaling.

Key words: prostate cancer, miR-29c, VEGF, VEGFR2