关键词: microRNA-574-5p, 宫颈癌, 增殖, 凋亡, 迁移

Abstract: Objective Investigate the expression level of tissue microRNA-574-5p (miR-574-5p) and the effects of down-regulation of miR-574-5p on SiHa cell; Methods The level of tissue miR-574-5p in 80 samples cervical cancer and 60 normal samples as controls were detected by real-time RT-PCR. Transfected the miR-574-5p inhibitor into SiHa cell, and detected the proliferation, the apoptotic rate and the migration of SiHa cell through MTT, the flow cytometer and transwell experiment respectively. Results Expression levels of tissue miR-574-5p was significantly higher in cervical cancer than that in normal controls（P＜0.001); Up-regulation expression of miR-574-5p had a significantly positive correlation with the International Federation of Gynecology and Obstetrics (FIGO) stage and pathological grade and lymph node metastasis (P＜0.05 ), while it had no clear-cut correlations with the age of patients and menopausal status. After transfected the miR-574-5p inhibitor into SiHa cell, the proliferation and the migration were decline（P＜0.05）, and the apoptotic rate was addition(P＜0.05). Conclusion The high expression of miR-574-5p in the tissue of cervical cancer may be related to the progress in the malignant cervical cancer, which expected to be a potential targeting therapy of cervical cancer through the effects of transfecting miR-574-5p inhibitor on SiHa cell.

Key words: MicroRNA-574-5p, Cervical cancer, proliferation, apoptotic, migration