关键词: 虫草素, 顺铂, Eca109, 细胞凋亡, NF-κB, Bcl-2, Bax

Abstract: Objective To investigate the mechanism of action and impact of apoptosis in Eca109 treated with cordycepin and cisplatin METHODS Divide Eca109 into four groups named control group,cordycepin,cisplatin and combined group which was treated with different concentrations of cordycepin,cisplatin and cordycepin（70μg/mL）and cisplatin（0.8μg/mL）.The proliferation was measured by MTS assay. Apoptosis was observed by using Hoechst 33258 staining and flow cytometry. Western blotting was used to detect the expression of NF-κBp65,Bcl-2 and Bax. Enzyme linked immunosorbent assay(ELISA) was used to detct the binding activity with NF-κBP65 and DNA in cell nucleus.RESULTS The inhibition ratio induced by cordycepin and cis-platinum combination was increased from 29.3% to 70.4% in Eca109 cells（P＜0.05）. Cordycepin combined with cis-platinum can remarkablely promote apoptosis in Eca109 cells than using cisplatin alone.Cisplatin increased the activity of the NF-κBP65 in the nuclei while cordycepin can inhibit the activity of the NF-κB P65, the activity of the NF-κB P65 decreased with combined treatment of cordycep and cisplatin. Compared to cisplatin alone，cordycepin combined with cisplatin decreased the expression of Bcl-2 while remarkblely increased the expression of Bax. CONCLUSION Cordycepin could enhanced the apoptosis of Eca109 cells induced by cisplatin and regulated downstream signaling molecule expression of Bcl-2 and Bax by inhibiting the NF-κB pathway.

Key words: cordycepin, cisplatin, Eca109, apoptosis, NF-κB, Bcl-2, Bax