关键词: 关键词：SELEX技术, HIV-p24, 核酸适配体

Abstract: Abstract: Objective Aptamers to HIV-p24 were screened through SELEX technique for the diagnosis and therapy of AIDS. Methods With recombinant p24 for the screening target, oligonucleotides binding HIV-p24 were screened from a random oligonucleotide library through SELEX technique. The binding capacity between oligonucleotides obtained from the 12th round of screening and HIV-p24 was identified via Electrophoretic Mobility Shift Assay (EMSA).The aptamers strongly binding to HIV-p24 were screened and the specificity of aptamers recognizing HIV-p24 was detected by Dot-blot method. Results 5 aptamers with strong binding capacity to HIV-p24 were obtained and are different sequences. The binding speci?city indicated that No.18 and No.26 apatmers only bond with HIV-p24, not with human serum albumin, bovine serum albumin and skimmed milk powder. Conclusion 2 aptamers specially binding to HIV-p24 were obtained, this study provides an experimental basis for the diagnosis and treatment of AIDS by utilizing aptamer of HIV-p24.

Key words: Key words: SELEX technique, HIV-p24, aptamer