关键词: 羧胺三唑(CAI), 小胶质细胞, 肌萎缩侧索硬化症(ALS）, 肿瘤坏死因子(TNF-α)

Abstract: Objective This study is designed to explore the regulation of carboxyamidotriazole (CAI) on inflammatory factors of BV2 cell model Methods BV2 cells were incubated with different concentrations of CAI(5~40μmol/L), LPS was then added and cells were incubated for 18 h, TNF-α, IL-1β, IL-6 levels were determined with ELISA kits and NO levels were determined with Griess reagent, respectively the mRNA levels of TNF-α, IL-1β, COX-2 and iNOS were detected by Real-time Quantitative PCR Results The viability of BV2 cells was not affected at the concentrations of CAI used (5~40μmol/L) , CAI was found to reduce the levels of TNF-α products (359.4 ±12.9 vs241.6 ±16.1*,135.7 ±6.4**,5.3 ±1.9**pg/ml, p<0.01) and IL-1β, NO, IL-6 products (p<0.01) in a dose-depended manner at the concentrations 20,30 and 40μmol/L; CAI was also found to inhibit the mRNA expression of TNF-α，IL-1β，COX-2 and iNOS Conclusion The present study suggests that CAI has obvious effect on TNF-α，IL-1β，NO，IL-6 secretions of BV2 cells model, and CAI was found to has a remarkable inhibitory effect on the activation and inflammatory response of the LPS-induced BV2 cells

Key words: carboxyamidotriazole (CAI), micoglia, amyotrophic lateral sclerosis（ ALS）, Tumor necrosis factor-α（TNF-α）