急性冠脉综合征合并2型糖尿病患者循环微粒致大鼠血管内皮细胞损伤

doi:10.16352/j.issn.1001-6325.2026.03.0381

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (3): 381-387.doi: 10.16352/j.issn.1001-6325.2026.03.0381

急性冠脉综合征合并2型糖尿病患者循环微粒致大鼠血管内皮细胞损伤

常凤军¹, 王星^2*, 杨禹娟¹, 李红文^1*

1.陕西省人民医院心内科,陕西西安 710068;
2.蓝田县人民医院心内科,陕西西安 710500

收稿日期:2025-04-23 修回日期:2025-07-14 出版日期:2026-03-05 发布日期:2026-02-25
通讯作者: ^*772970577@qq.com;18792916588@163.com
基金资助:
陕西省自然科学基金(2020JM-666)

Circulating microparticles from acute coronary syndrome patients complicated with type 2 diabetes induce endothelial cell injury in rats

CHANG Fengjun¹, WANG Xing^2*, YANG Yujuan¹, LI Hongwen^1*

1. Department of Cardiology, Shaanxi Provincial People′s Hospital, Xi′an 710068;
2. Department of Cardiology, Lantian County People′s Hospital, Xi′an 710500, China

Received:2025-04-23 Revised:2025-07-14 Online:2026-03-05 Published:2026-02-25
Contact: ^*772970577@qq.com;18792916588@163.com

摘要/Abstract

摘要： 目的探究急性冠状动脉综合征(ACS)合并2型糖尿病(T2DM)患者的循环微粒(MPs)对血管内皮的损伤作用。方法提取对照组(control,n=15)、ACS患者(ACS组,n=18)、T2DM患者(T2DM组,n=18)以及ACS合并T2DM患者(ACS+T2DM组,n=18)的MPs。用MPs孵育大鼠胸主动脉后,生物法测定MPs对内皮依赖性血管舒张的影响;Western blot检测小窝蛋白-1(caveolin-1)的表达、内皮型一氧化氮合酶(eNOS)与热休克蛋白90(Hsp90)的偶联;化学法测定一氧化氮(NO)和超氧阴离子的变化。结果 ACS+T2DM患者的MPs浓度高于对照组(P＜0.05);但与ACS、T2DM患者比较,3组间MPs浓度差异无统计学意义。与对照组比较,ACS、T2DM和ACS+T2DM患者,其MPs均抑制内皮依赖性血管舒张功能、eNOS与Hsp90的偶联及NO的产生,而刺激caveolin-1蛋白的表达和的生成(P＜0.05)。然而,与ACS、T2DM患者比较,ACS+T2DM患者的MPs的上述作用更强(P＜0.05)。结论 ACS合并T2DM患者的MPs的质量(或功能)改变,导致MPs对离体大鼠血管内皮的损伤。MPs具有作为内皮功能受损靶标的潜质。

关键词: 急性冠状动脉综合征, 循环微粒, 内皮功能障碍, 血管损伤, 氧化应激

Abstract: Objective To investigate the effects of circulating microparticulates (MPs) on vascular endothelium injury in patients with acute coronary syndrome (ACS) and type 2 diabetes (T2DM). Methods MPs were extracted from control (n=15), ACS patients (ACS, n=18), T2DM patients (T2DM, n=18) and ACS patients with T2DM (ACS+T2DM group, n=18). After incubation of rat thoracic artery tissue with MPs, the effects of MPs on endothelial dependent vasodilatation was detected with biological assay, expression of caveolin-1, coupling of endothelial nitric oxide synthase (eNOS) with heat shock protein 90 (Hsp90) was detected with Western blot. The changes in nitric oxide (NO) and superoxide anion were examined with chemical assay. Results The MPs concentration in ACS+T2DM patients samples was higher than that in control (P＜0.05). However, compared with ACS and T2DM patients, there was no statistically significant difference in MPs concentration. Compared with the control, regardless of ACS, T2DM and ACS+T2DM, their MPs inhibited endothelial dependent vasodilatation function, eNOS and Hsp90 coupling and NO production and stimulated expression of caveolin-1 and generation(P＜0.05). However, compared with ACS and T2DM patients, the effects of MPs were stronger in ACS+T2DM(P＜0.05). Conclusions The changes in the quality (or function) of MPs in ACS+T2DM lead to damage of rat vascular endothelium by MPs. MPs are potential marker molecules to support diagnosis of endothelial dysfunction.

Key words: acute coronary syndrome, circulation microparticles, endothelial dysfunction, vascular injury, oxidative stress

中图分类号:

常凤军, 王星, 杨禹娟, 李红文. 急性冠脉综合征合并2型糖尿病患者循环微粒致大鼠血管内皮细胞损伤[J]. 基础医学与临床, 2026, 46(3): 381-387.

CHANG Fengjun, WANG Xing, YANG Yujuan, LI Hongwen. Circulating microparticles from acute coronary syndrome patients complicated with type 2 diabetes induce endothelial cell injury in rats[J]. Basic & Clinical Medicine, 2026, 46(3): 381-387.

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急性冠脉综合征合并2型糖尿病患者循环微粒致大鼠血管内皮细胞损伤

Circulating microparticles from acute coronary syndrome patients complicated with type 2 diabetes induce endothelial cell injury in rats

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