急性缺血性卒中患者血栓中巨噬细胞数量与中性粒细胞胞外陷阱网含量相关

doi:10.16352/j.issn.1001-6325.2026.02.0220

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (2): 220-226.doi: 10.16352/j.issn.1001-6325.2026.02.0220

急性缺血性卒中患者血栓中巨噬细胞数量与中性粒细胞胞外陷阱网含量相关

臧明明^1,2, 韩若东², 颜秀侠^1,2*

1.蚌埠医科大学研究生院,安徽蚌埠 233000;
2.安徽医科大学附属亳州医院重症医学科二病区,安徽亳州 236000

收稿日期:2025-03-10 修回日期:2025-06-27 出版日期:2026-02-05 发布日期:2026-01-21
通讯作者: ^* xiaohan976@126.com
基金资助:
安徽省高校自然科学研究重点项目(2022AH050688);亳州市卫生健康科研一般项目(bzwj2023c004)

Association between macrophage count and neutrophil extracellular trap content in thrombi of acute ischemic stroke patients

ZANG Mingming^1,2, HAN Ruodong², YAN Xiuxia^1,2*

1. Graduate School, Bengbu Medical University, Bengbu 233000;
2. Department of Critical Care Medicine, the Second Ward, Bozhou Hospital, Anhui Medical University, Bozhou 236000,China

Received:2025-03-10 Revised:2025-06-27 Online:2026-02-05 Published:2026-01-21
Contact: ^* xiaohan976@126.com

摘要/Abstract

摘要： 目的探究急性缺血性卒中(AIS)患者血栓中巨噬细胞和中性粒细胞胞外陷阱网(NETs)含量的相关性及不同亚型巨噬细胞对NETs的降解和清除能力。方法通过免疫组化染色检测AIS患者血栓中的CD16巨噬细胞、CD163巨噬细胞及瓜氨酸化组蛋白3(CitH3);通过检测血栓-巨噬细胞共培养后CitH3浓度评估不同亚型巨噬细胞对血栓中NETs的降解和清除能力。结果 30例行免疫组化染色的血栓均有CD16巨噬细胞、CD163巨噬细胞及CitH3阳性表达,血栓中CD16阳性细胞数和CD163阳性细胞均与CitH3阳性面积百分比呈正相关(CD16:r=0.538, P＜0.01; CD163: r=0.641, P＜0.001);对共培养后CitH3浓度进行t检验发现,与空白对照组相比,血栓-M1和M2巨噬细胞组CitH3浓度均偏低(M1:t=-3.981, P＜0.001; M2:t=-2.057, P＜0.05),且血栓-M1巨噬细胞组CitH3浓度相较于血栓-M2巨噬细胞组更低(t=-2.125, P＜0.05)。结论 AIS患者血栓中M1巨噬细胞和M2巨噬细胞数量与NETs含量相关,且不同亚型巨噬细胞对NETs的清除和降解能力存在差异。

关键词: 急性缺血性卒中, 中性粒细胞胞外陷阱网, 巨噬细胞, 血栓

Abstract: Objective To explore the correlation between the number of macrophages and neutrophil extra cellular traps (NETs) in thrombi of patients with acute ischemic stroke (AIS), and to compare the NET-degrading and clearing abilities of macrophages in different polarization states. Methods Immunohistochemical staining was performed to detect CD16⁺ macrophages, CD163⁺ macrophages and citrullinated histone 3(CitH3) in AIS thrombi. Thrombi were co-cultured with macrophages, and the CitH3 concentration in the supernatant was measured to evaluate the NET-degrading and clearing capacities of different macrophage subtypes. Results All 30 thrombi showed positive expression of CD16 macrophages, CD163 macrophages and CitH3. The numbers of both CD16⁺ and CD163⁺ macrophages were positively correlated with the percentage of CitH3-positive area (CD16: r=0.538, P＜0.05; CD163: r=0.641, P＜0.001). After co-culture, the CitH3 concentration was significantly lower in both the M1- and M2- macrophage groups than in the blank control group (M1: t=-3.981, P＜0.001; M2: t=-2.057, P＜0.05). The concentration of CitH3 was lower in the M1 group than in the M2 group (t=-2.125, P＜0.05). Conclusions Both M1 and M2 macrophage infiltration are correlated with NET content in AIS thrombi, and their abilities to clear and degrade NETs are different.

Key words: acute ischemic stroke, neutrophil extra cellular traps, macrophages, thrombus

中图分类号:

R743

臧明明, 韩若东, 颜秀侠. 急性缺血性卒中患者血栓中巨噬细胞数量与中性粒细胞胞外陷阱网含量相关[J]. 基础医学与临床, 2026, 46(2): 220-226.

ZANG Mingming, HAN Ruodong, YAN Xiuxia. Association between macrophage count and neutrophil extracellular trap content in thrombi of acute ischemic stroke patients[J]. Basic & Clinical Medicine, 2026, 46(2): 220-226.

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急性缺血性卒中患者血栓中巨噬细胞数量与中性粒细胞胞外陷阱网含量相关

Association between macrophage count and neutrophil extracellular trap content in thrombi of acute ischemic stroke patients

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