以癫首发的颅内海绵状血管瘤患儿内皮细胞来源外泌体细胞间黏附分子-1表达研究

doi:10.3969/j.issn.1672-6731.2020.11.013

摘要/Abstract

摘要：

目的探讨外泌体细胞间黏附分子-1（ICAM-1）在颅内海绵状血管瘤（CCM）相关性癫患者的表达变化及其机制。方法收集2014年2月至2019年2月确诊的儿童癫患者临床资料，依据有无合并CCM分为癫组和CCM组，并以同期体格检查的健康儿童为对照，每组各20例。通过磁珠捕获技术分选血清外泌体，流式细胞术检测外泌体表面ICAM-1表达；ELISA检测血清ICAM-1含量；免疫组化检测组织内ICAM-1表达变化和CD68⁺巨噬细胞数目。结果对照组、癫组和CCM组受试者血清ICAM-1含量差异无统计学意义（P>0.05）；对照组与癫组受试者血清CD31⁺外泌体ICAM-1平均荧光强度差异无统计学意义（P>0.05），但CCM组患儿血清CD31⁺外泌体ICAM-1平均荧光强度较癫组增加85.60%[（24.77 ±3.90）%对（45.97 ±5.06）%；t=3.317，P=0.008]；海绵状血管瘤组织中ICAM-1平均光密度与巨噬细胞数目呈正相关（r_s=0.909，P=0.001）；与对照组相比，共培养组外泌体表面ICAM-1平均荧光强度增加25.61%[（164.81 ±7.00）%对（207.03 ±9.18）%；t=3.652，P=0.004]，脂多糖组则进一步增加，相对于共培养组增加71.13%[（354.31 ±18.22）%对（207.03 ±9.18）%；t=7.212，P=0.000]，采用白细胞介素-6（t=4.570，P=0.001）和肿瘤坏死因子-α（t=5.105，P=0.000）阻断后外泌体ICAM-1表达水平部分降低。结论 CD31⁺外泌体ICAM-1在CCM相关性癫患儿体内呈高表达，巨噬细胞与内皮细胞来源外泌体ICAM-1表达变化具有较大的相关性。

关键词: 血管瘤, 海绵状, 中枢神经系统, 癫, 内皮细胞, 外泌体, 细胞黏附分子, 儿童

Abstract:

Objective To investigate the expression and mechanism of exosomal intercellular adhesion molecule-1 (ICAM-1) in patients with intracranial cavernous hemangioma (CCM) associated epilepsy. Methods Clinical data of children with epilepsy diagnosed from February 2014 to February 2019 were collected and divided into epilepsy group and CCM group according to the presence or absence of CCM. Healthy children who underwent physical examination during the same period were used as the control group. There were 20 cases in each group. The secretions in serum were sorted by magnetic beads, and ICAM-1 expression was detected by flow cytometry. Serum ICAM-1 was detected by enzyme-linked immunsorbent assay (ELISA). The expression of ICAM-1 and the number of CD68⁺ macrophages were detected by immunohistochemistry. Results The results of ELISA showed that there was no significant difference in serum ICAM-1 concentration among the control group, epilepsy group and CCM group (P>0.05). There was no significant difference in the average fluorescence intensity of CD31⁺ exosome ICAM-1 between the control group and the epilepsy group. Compared with the epilepsy group, the average fluorescence intensity of CD31⁺ exosome ICAM-1 in the CCM group increased by 85.60%[(24.77 ±3.90)% vs. (45.97 ±5.06)%; t=3.317, P=0.008]. The average optical density of ICAM-1 in CCM tissue was positively correlated with the number of macrophages (r_s=0.909, P=0.001). The results of co-culture experiment showed that compared with the control group, the average fluorescence intensity of ICAM-1 on the exosome surface of co-culture group increased by 25.61%[(164.81 ±7.00)% vs. (207.03 ±9.18)%; t=3.652, P=0.004]; the average fluorescence intensity of ICAM-1 on the exosome surface of LPS group further increased by 71.13%[(354.31 ±18.22)% vs. (207.03 ±9.18)%; t=7.212, P=0.000]; the blockade of IL-6 (t=4.570, P=0.001) and TNF-α (t=5.105, P=0.000) could partly reduce the expression of ICAM-1 on the exosome. Conclusions The expression of CD31⁺ exosome ICAM-1 is significantly increased in patients with CCM associated epilepsy. There is a strong correlation between the expression of macrophages and endothelial cell-derived exosome ICAM-1.

Key words: Hemangioma, cavernous, central nervous system, Epilepsy, Endothelial cells, Exosomes, Cell adhesion molecules, Child

李斌, 周辉, 金爱琴. 以癫首发的颅内海绵状血管瘤患儿内皮细胞来源外泌体细胞间黏附分子-1表达研究[J]. 中国现代神经疾病杂志, 2020, 20(11): 1008-1013.

LI Bin, ZHOU Hui, JIN Ai-qin. Clinical study of intercellular adhesion molecule-1 expression in endothelial-derived exosome in children with intracranial cavernous hemangioma with epilepsy[J]. Chinese Journal of Contemporary Neurology and Neurosurgery, 2020, 20(11): 1008-1013.

http://journal11.magtechjournal.com/cjcnn/CN/Y2020/V20/I11/1008

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2 以癫首发的颅内海绵状血管瘤患儿内皮细胞来源外泌体细胞间黏附分子-1表达研究

Clinical study of intercellular adhesion molecule-1 expression in endothelial-derived exosome in children with intracranial cavernous hemangioma with epilepsy

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2 以癫首发的颅内海绵状血管瘤患儿内皮细胞来源外泌体细胞间黏附分子-1表达研究

Clinical study of intercellular adhesion molecule-1 expression in endothelial-derived exosome in children with intracranial cavernous hemangioma with epilepsy

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