摘要： 采用经枕大池注射自体血方法制备蛛网膜下隙出血兔模型，光学显微镜和电子显微镜下观察神经元凋亡并计算凋亡指数。组织病理学观察神经元呈现延迟性损伤，胞体固缩，胞质及胞核深染，核质不均匀，胞核内染色质部分凝聚；甚至胞核溶解，线粒体肿胀、嵴消失。与空白对照组相比，蛛网膜下隙出血组动物细胞凋亡指数明显升高，且组间差异具有统计学意义（均P < 0.05）。提示细胞凋亡在蛛网膜下隙出血后的迟发性神经元死亡过程中起重要作用。

关键词: 蛛网膜下腔出血, 细胞凋亡, 神经元, 疾病模型, 动物

Abstract: Fresh autologous blood was injected into the cisterna magna to establish the model of spontaneous subarachnoid hemorrhage (SSAH) in the rabbit groups. The neuron apoptosis was observed by optical microscope and eletron microscope, and determination in apoptoic index. Observation by histopathology, neurons show tardive detriment, as cell body pyknosis, cytoplasm and nucleus hyperchromasis, karyoplasms uneven, fraction intranuclear caryotin agglomeration; even caryolysis, bioblast engorgement, crista resorbed. Contrast to blank groups, apoptotic index increased obviously in SAH groups, and statistical significant difference was seen among groups (all P < 0.05). Apoptosis plays an important role in delayed neuronal death after subarachnoid hemorrhage.

Key words: Subarachnoid hemorrhage, Apoptosis, Neurons, Disease models, animal