Table of Content

    05 February 2014, Volume 34 Issue 2
    HO-1 transduced by MSCs promotes angiogenesis in rat infracted myocardium
    2014, 34(2):  145-150. 
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    Objective: Heme oxygenase-1 (HO-1) is a stress-inducible enzyme with diverse cytoprotective effects, and is reported a role for angiogenesis rencently. We investigated whether HO-1 transduced by MSCs can induce angiogenic effects in infracted myocardium. Methods HO-1 was transfected into cultured MSCs using an adenoviral vector. 1×106 Ad-HO-1-transfected MSCs (HO-1-MSCs) or Ad-Null-transfected MSCs (Null-MSCs) or PBS was respectively injected into rat hearts 1 h intramyocardially after myocardial infarction. Results HO-1-MSCs was able to induce stable expression of HO-1 in Vitro and Vivo. The capillary density and expression of angiogenic growth factors,VEGF and FGF2, were significantly enhanced in HO-1-MSCs-treated hearts compared with Null-MSCs-treated and PBS-treated hearts. However, the angiogenic effects of HO-1 in HO-1-MSCs group could be abolished by treating the animals with HO inhibitor, zinc protoporphyrin. The myocardial apoptosis was marked reduced with significantly reduced fibrotic area in HO-1-MSCs-treated hearts; Furthermore, the cardiac function and remodeling were also significantly improved in HO-1-MSCs-treated hearts than in the other two groups. Conclusion HO-1 modification with MSCs reduces the apoptosis of myocardium and preserves cardiac function and remodeling, and that this is associated with siginficant angiogenesis.
    The expression of DNA methyltransferases 1, 3A and 3B in the brain of hypoxic preconditioned mice
    2014, 34(2):  151-154. 
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    Objective To study the changes of DNA methyltransferases (DNMT) in acute repeated hypoxic mice brain. Methods Mice were randomly divided into control group (H0), hypoxia group (H1) and hypoxia for 4 runs group (H4). Real-time PCR, Western blot and methyltransferase activity assay were adopted to detect the changes of three kinds of DNMTs. Results mRNA and protein levels of DNMT3A and DNMT3B were found to be decreased in H1 and H4 (p<0.05,vs H0). The activity of DNA methyltransferase was decreased in H4(p<0.05,vs H0 and H1). Conclusion The changes of DNA methyltransferase may be closely related to protection of the brain in hypoxic preconditioned mice.
    Effects of rhein lysinate and cisplatin on proliferation inhibition and apoptosis inducing of lung cancer A549 cells
    2014, 34(2):  155-159. 
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    Objective To investigate the effect of Rhein lysinate (RHL), cisplatin and their combination on the proliferation, apoptosis of lung cancer A549 cells, and to provide theoretical foundation for cisplatin combined with RHL against human lung cancer. Methods The lung cancer A549 cells in logarithm growth phase were selected as the research object, and were randomly divided into four groups: blank control group, cisplatin group, RHL group and combination groups. MTT assay was used to detect A549 cell proliferation and flow cytometry was used to analyze cell apoptosis in various groups after 48 h. The expression levels of protein associated with apoptosis and protein of ERK were detected after 48 h by Western blotting. Results Cell proliferation was inhibited and cell apoptosis was induced in groups of cisplatin and RHL, but there was a significant reduction in proliferation and a significant increase in apoptosis in A549 cells treated with a combination of cisplatin and RHL compared with cisplatin or RHL treatment alone (P<0.05, respectively). The expressions of cleaved caspase-3, caspase-7 and poly ADP-ribose polymerase (PARP) in combination groups were higher than those of either agent alone group after 48 h cell culture in A549 cells, whereas the expression of BCL-2 was lower than those of either agent alone group and the expressions of BAX was higher than those of either agent alone group. Moreover, RHL could downregulate the phosphorylation of ERK induced by cisplatin. Conclusion RHL potentiates the cisplatin-induced cytotoxicity and apoptosis by reducing the activation of ERK and increasing the levels of cleaved caspase-3, caspase-3 and PARP.
    Proteasome inhibitor MG132 improves the renal tubule-interstitium in diabetic rats
    2014, 34(2):  160-167. 
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    Objective To investigate proteasome inhibitor MG132 whether or not to reduce or slow down the renal tubule-interstitium during diabetic nephropathy (DN). Methods The diabetic rat model(DM) was established by injecting Streptozotocin(STZ) (n = 10). Meanwhile, ten rats were grouped into normal control group (NC). After 24 weeks, the rats were sacrificed to detect relevant biochemical parameters, and to observe the changes of pathomorphology of kidney and pancreas as well. NRK-52E cells were cultured in vitro, to be pre-treated with different doses MG132 cultured in high glucose. In addition, immunohistochemistry and immunofluorescence staining, Western blotting were employed to detect the protein expression of Smad7, Smad ubiquitin regulatory factor 2(Smurf2), E-cadherin (E-cadherin) and α-smooth muscle actin (α-SMA) and fibronectin (FN), collagen-Ⅰ (Col-1)in the renal tissue and NRK-52E cells. Results Compared with NC group,the expression of E-cadherin decreased and α-SMA increased in DM group (P <0.05). In DM group, the expressions of FN and Col-1 in renal interstitium were increased (P < 0.05), while the protein expression of Smad7decreased (P <0.05),but Smurf2 was increased (P <0.05). In vitro, MG132 inhibited the protein expressions of α-SMA and Col-Ⅰby a dose-dependent manner which were induced by high glucose in NRK-52E cells (P <0.05), but it had no effect with the protein expression of Smurf2.In contrary, MG132 increased the protein expression of Smad7 and E-cadherin (P <0.05). Conclusion MG132 inhibited the protein degradation of Smad7 which high glucose-mediated, could reduce the development of the renal tubule-interstitium, this may be one of the mechanisms that MG132 could treat DN.
    Atorvastatin down regulating mitofusin 2 gene expression and cell apoptosis
    2014, 34(2):  168-171. 
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    Objective To investigate the effects of atorvastatin on myocardial apoptosis and mitofusin 2(Mfn2) expression after acute myocardial infartion(MI) in rats. Methods A total of 48 male Sprague-Dawley rats were randomly divided into sham-operated group, myocardial infartion(MI) group, statin group 1 and group 2. The rats in the two statin groups were given atorvastatin 10 mg?kg-1 or 40 mg?kg-1 daily, respectively. The rats in the MI and statin groups were induced acute myocardial infartion. 24 hours after operation, the MI areas of rat hearts were harvested, and myocardial apoptosis was assessed by in situ terminal deoxynucleotidyl transferase(TdT)-dUTP nick-end labeling(TUNEL staining). Immunohistochemistry staining and western blotting were performed to measure the expression of Mfn 2 and phosphorylated Akt(p-Akt) . Results On the 24 hours after operation, compared with those of the sham group, the myocardial apoptosis and Mfn2 protein expression of the MI groups remarkably increased(p<0.01). Compared with those of the MI group, myocardial apoptosis and Mfn2 protein expression of statin groups significantly decreased with a slightly dose-dependent manner(p<0.05). Conclusion Atorvastatin attenuates myocardium apoptosis after acute myocardial infarction in rats, which may relate to down-regulated expression of mitofusin 2.
    Establishment and primary application of a ALU-based bDNA for the detection of plasma circulating free DNA in acute myocardial infarction
    2014, 34(2):  172-175. 
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    Objective To establish a ALU-based branched DNA (bDNA) method for detection of human plasma circulating free DNA (cf-DNA) and investigate its concentration in acute myocardial infarction (AMI). Methods Based on the sequence of ALU, a bDNA method was established, Liner, sensitivity and reproducibility were evaluated; according to the standard curve created, the concentration of ALU in AMI were determined and the relationship of cf-DNA concentrations with cardiac enzymes were presented. Results The linear rang was 0-400 ng/mL, the coefficient correlation was 0.99,the intra-assay coefficient variation was 7.85%-11.75%, the inter-assay coefficient variation was10.05%-14.32%. The concentration of ALU in AMI and healthy controls were 4223 ng/mL and 118 ng/mL (P<0.001) respectively indicating that ALU concentration was increased in AMI. No correlation was found among ALU and cardiac enzymes. The area under ROC was 0.99, the sensitivity was 98.8% and the specificity was 100.0%. Conclusions The ALU-based bDNA method is sensitive and reproductive; cf-DNA concentration is associated with AMI.
    Nine genetic characters of Mongolian’s head face in Yunnan province
    2014, 34(2):  176-178. 
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    Objective To understand the genetic characters ofMongolian,s head face.Methods Nine genetics indexes, includingmongoloid fold, eyefold of the upper eyelid, front tooth type, nasal profile, nostril form, chin projection, lobe type, hair pointof the forehead and hair form were investigated for208 middle schol students(105 males and 103 females) inMongolian nationality by using cluster random samplingmethod.Results (1)The rate ofMongoliafold is 89.5%, the rate of eyefold of the upper eyelid is 91.4%, the rate of shovel-shaped front tooth type is96?19%, the rate of umbonate nasal profile is32.4%, the rate of wide nostril is 80%, the rate of protrudingchin is 32.4%, the rates ofear lobe is76.2%, the rate ofpointed hairpointon forehead is21% and the rateof curly hair form is7.7% inMongolian nationality; (2) No significant sexual diffrernce was found in the frequencies of 7 genetics indexes ; (3)These nine indexes showed no correlations.Conclusion;(4)There are differencesbetweenMongolian nationality and the Mongolian groups in Inner Mongolia area.
    Jinmaitong Capsule Can Ameliorate the Peripheral Neuropathic Pain of STZ-DM Rats by Enhancing the Expressions of Nrf2 and HO-1 in DRG
    2014, 34(2):  179-184. 
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    Objective To study the effects of Jinmaitong Capsule (JMT) on the expressions of nuclear factor-E2-related factor 2 (Nrf2) and haem oxygenase-1 (HO-1) and their mRNAs in dorsal root ganglion (DRG) and the level of plasma carbon monoxide (CO) in STZ-induced diabetic rats. Method STZ-induced diabetic rats were randomly divided into 5 groups including model group, low-dose JMT group, middle-dose JMT group, high-dose JMT groupand Thioctic Acid Capsule (TAC) group. Ten normal rats matching with weight and age served as the control group. All rats were given intragastric administration for 12 weeks and then culled. The mechanical withdrawal threshold was tested by electronic Von Frey instrument before death. The expressions of Nrf2 and HO-1 and their mRNAs in DRG were detected by SABC immunohistochemical method and real-time fluorogenetic quantitative PCR respectively. And the content of plasma COHb was also measured. Result Compared with the control group, the mechanical withdrawal threshold, the content of plasma CO and the expressions of Nrf2 and HO-1 and their mRNAs in DRG of all the diabetic rats groups decreased significantly (p<0.01). All the above-mentioned indexes in treatment groups raised strikingly (p<0.05 or p<0.01). The middle-dose JMT group was more effective than the TAC group in enhancing the expression of HO-1 in DRG (p<0.05). Conclusion Jinmaitong Capsule can ameliorate the peripheral neuropathic pain of STZ-DM rats by enhancing the expressions of Nrf2, HO-1 and CO in DRG.
    Autophagy inhibitor contributes to apoptosis induced by anoxia in cardiac myocytes
    2014, 34(2):  185-189. 
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    Objective This study aims to investigate the potential roles of autophagy induced by ischemia in effecting on expression of Bim and caspase-3 and tries to clarify the regulation of HIF-1 in this process. Methods We employed simulated anoxia of neonatal rat ventricular myocytes as an in vitro model of ischemia injury to the heart. Cardiac myocytes were exposed to 0,2,4,8,14 and 24h anoxia. Cardiac myocytes were pretreated with 10mmol/L 3-Methyladenine (3MA) to inhibit autophagy. The beating rate and arrhythm of myocardial cells were detected by inverted microscope. The activity of lactate dehydrogenase was determined by automatic biochemistry analyzer. Western blot analysis was used to examine variation in the expression of LC3-II/I (a marker of autophagy),Bim,caspase-3and HIF-1. Results As the beating rate of myocardial cells was slightly decreased, and the cell arrhythm was increased (P<0.05) after inhibition autophagy; the activity of lactate dehydrogenase was increased (P<0.05)when inhibiting autophagy. Moreover, significant autophagy was reduced by pretreating with 3-Methyladenine (3MA) in anoxia, and the expression of Bim and caspase-3 was significantly increased (P< 0.05). The expression of HIF-1was increased significantly in anoxia group and reduced in anoxia+3MA group (P< 0.05). Conclusion Inhibition of autophagy constitutes a powerful and previously uncharacterized apopotosis induced by anoxia in cardiac myocytes via upregulating the pro-apopototic protein Bim and caspase-3. And HIF-1 positively regulated the process of autophagy induced by anoxia.
    The influence of of PTEN on VEGF and MMP expression in myeloid leukemia cells
    2014, 34(2):  190-195. 
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    Objective To investigate the influence and relationship of tumor-suppressing gene PTEN (phosphatase and tensin hemology deleted on chromosome ten gene), VEGF(vascular endothelial growth factor) and MMP (matrix metalloproteinase) in myeloid leukemia. Methods 1)To explore the PTEN, VEGF, MMP-2 and MMP-9 mRNA expression levels on 10 chronic myelogenous leukemia patients in chronic phase (CML-CP), 10 CML patients in blast crises (CML-BC), 10 normal control marrow mononuclear cells (MMNC). 2)The recombinated adenovirus containing green fluorescent protein (GFP) and PTEN(Ad-PTEN-GFP)or empty vector (Ad-GFP)was transfected into human leukemia K562 cells. The growth inhibition ratio of K562 cells were observed by MTT assay; PTEN, VEGF, MMP-2 and MMP9 mRNA levels were detected by real-time fluorescent relative-quantification reverse transcriptional PCR (FQ-PCR). PTEN, VEGF and p-Akt protein levels were detected by western blot, MMP-2 and MMP-9 protein were detected by gelatin zymogram. Results: The mRNA expression levels of PTEN in CML-BC patients were lower than CML-CP patients(P<0.05). But VEGF, MMP-2 and MMP-9 mRNA levels were reverse compared with PTEN(P<0.05). VEGF, MMP-2 and MMP-9 mRNA expression levels were down regulated about 4.80、5.88、5.72 fold; p-Akt, VEGF, MMP-2 and MMP-9 proteins were also down-regulated 26.0, 5.23, 2.86, 4.76 fold compared with Ad-GFP group; PTEN mRNA expression levels were negative with VEGF, MMP-2, MMP-9 mRNA after K562 cells transfected with wild type PTEN of MOI=200 after three days. Conclusion PTEN might have anti-angiogenesis and anti-adhesion ability in myeloid leukemia via inhibition VEGF, MMP-2 and MMP-9 expression.
    Extended-spectrum β-lactamases genotypes and homologous analysis of Shigella flexneri isolates
    2014, 34(2):  196-200. 
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    Objective To analyze the resistant pattern and the extended-spectrum β-lactamase genotypes of clinical isolates and homologous analysis of Shigella flexneri in Tangshan. Methods Susceptibility testing was perfomed by K-B disk diffusion method ESBLs confirmation experiment. The MICs against ESBLs-producting were determined by trace broth dilution method..PCR method was employed to detect the genotypes of ESBLs producing strains. The PCR products were analyzed by DNA sequencing. Through the variable number tandem repeats (VNTR) homology analysis. Results ESBLs were idenitified in 44.2%(19/43) of Shigella flexneri isolates. So did MICs. 95~100% of these isolates were susceptible to ceftriaxone, cefotaxime, cefoperazone, Piperacillin, amoxicillin- clavulanate.More than 84% these isolates were susceptible to other antibiotics. The genotypes of these stains included CTX-M-14, CTX-M-3、CTX-M-57 and TEM-1. Homology analysis 19 strains of shigella's blessing is divided into four clones.Conclusions The Shigella flexneri isolates in Tangshan were multi-drug resistant. It is increasing on the third generation cephalosporin antibiotic. The main genotypes of ESBLs were CTX-M-14. The first detected CTX-M-57 genotype.Both genetic-related clones and non-related clones of Shigella flexneri in Tangshan.
    1α,25(OH) 2D3 Inhibits Gastric Cancer Cell Proliferation by Arrest Cell Cycle
    2014, 34(2):  201-205. 
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    Objective To observe the effect of 1α,25(OH)2D3 on gastric carcinoma cell proliferation and cell cycle progression and to investigate its possible mechanism.Methods Two gastric cancer cell lines BGC-823 and SGC-7901 were treated with 1α,25(OH)2D3 in a dose dependent manner. Cell proliferation was determined by methyl thiazolyl tetrazolium MTT assay; the cell cycle changes were detected by using flow cytometry; the expression of genes of P21, cyclinD1, cyclinE1 and CDK6 related to the cell cycle was analyzed by RT-PCR. Results 1α,25(OH) 2D3 significantly inhibited two gastric cancer cell lines proliferation in a dose dependent fashion, gastric cancer cells treated with 1α,25(OH) 2D3 caused significantly cell cycle arrest at G1 phase; RT-qPCR showed that 1α,25(OH)2D3 significantly increased P21 mRNA, and attenuated cyclinD1, cyclinE1, CDK6 mRNA expression in BGC-823, SGC-7901 cells(P<0.01). Conclusion 1α,25(OH)2D3 inhibited BGC-823, SGC-7901 cell proliferation and induced cell cycle arrest in G1 phase. The mechanism for the anti-proliferative and cell cycle arrest is probably related to overexpression of P21, down-regulated expression of cyclinD1, cyclinE1, CDK6.
    Hepatitis B virus(HBV) S gene-specific anti-gene locked nucleic acid(LNA) significantly inhibits S gene expression in vitro
    2014, 34(2):  206-210. 
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    Objective To investigate the inhibitory effects of hepatitis B virus(HBV) S gene-specific anti-gene locked nucleic acid(LNA) on HBV replication and expression in hepG2.2.15 cells. Methods The anti-gene LNA which were complementary to the purine rich region of the HBV S gene were designed,synthesized,and transfected by cationic liposomes into HepG2 2.2.15 cells. The HBsAg, HBeAg and HBV DNA of supernatants was tested by time-resolved fluorescence immune Assay(TRFIA) and real-time fluorescent quantitative polymerase chain reaction(FQ-PCR) at 2, 4, 6, 8 and 10 d after treatment. LNA’s cyto-toxicity on cell was evaluated by methyl thiazolyl tetrazolium(MTT) method. Results The anti-gene LNA that targeting on the purine rich region of HBV S gene showed strong inhibitory effects on replication of HBV DNA and the expression of HBsAg and HBeAg with the inhibition rates of 52.14%, 57.48% and 29.63% respectively after 6 days.There’s no obvious toxicity on cell. Conclusions Anti-gene locked nucleic acid that targeting on the purine rich region of HBV S gene has show strong inhibition on HBV in vitro.It has a therapeutic potential in the treatment of patients infected with HBV.
    Effects of eIF4H down-expression on the proliferation and erythroid differentiation of MEL cells
    2014, 34(2):  211-215. 
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    Objective To investigate the effect of eIF4H down-expression on the proliferation and erthroid differentiation of MEL cells. Methods The eIF4H-shRNA-1 or eIF4H-shRNA-2 plasmid, pCMV-VSVG and pCMV-dR8.2 were cotransfected into HEK293T cells to obtain Lentivirus. Lentivirus was infected into MEL cells to establish the stably eIF4H down-expressed MEL cells, Western blot was used to analyze the interference efficiency. Cells proliferation potential was measured by MTT assay; flow cytometry was used to detect the cell cycle; benzidine staining was applied to detect erythroid differentiation. Results Compared with the control, the expression of eIF4H was significantly down-regulated, the growth ability was significantly weaked, the percentage of cells in G1 phase significantly increased in eIF4H-shRNA-2 cells; benzidine-positive cells showed no difference between two group cells, but after sodium butyrate treatment, benzidine-positive cells was significantly increased in eIF4H-shRNA-2 cells. Conclusions We successfully established a stable eIF4H down-expressed MEL cells model; eIF4H down-expression can inhibit MEL cells proliferative capacity; MEL cells can not induce erythroid differentiation by eIF4H down-expression, but promote sodium butyrate-induced erythroid differentiation of MEL cells.
    Fasudil hydrochloride hydrate ameliorates myocardial fibrosis through inhibiting oxidative stress in rats with type 2 diabetes
    2014, 34(2):  216-221. 
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    Objective To deternine whether the RhoA/ROCK pathway is involved in the pathogenesis of myocardial fibrosis. Methods The experimental type 2 diabetic rats were established by high fat diet combined with one-time intraperitoneal injection of low dose streptozotocin (STZ).The rats were randomly divided into three groups: normal control group, diabetic group and fasudil group (intraperitoneal injection of fasudil 10 mg per kg every day, two injections).At week 24, The cardiac histological changes were observed by hematoxylin-eosin staining, transmission electron microscopy and masson staining. The volume of collagen was evaluated by hydroxyproline (HYP). The activities of the anti-oxidant enzymes (SOD) and malondialdehyde (MDA) in cardiac tissues were estimated by using commercially available kits.The eNOS activity in cardiac tissues was assessed by immunohistochemistry staining.The level of p-MYPT1 protein expression were examined by western blot. Results Compared to the control rats, the level of p-MYPT1 and the content of MDA were significantly elevated in the hearts of the diabetic group(both P<0.01),but the activities of SOD and the expression of eNOS were significantly lower than those of the NC group rats(both P<0.01). the concentrations of collagen concentration(MCC) in myocardial tissue of the DM group were higher(P<0.01). The fasudil group elevated the activities of SOD and the expression of eNOS (both P<0.01) but restrained the level of p-MYPT1 and the content of MDA(P<0.01,P<0.05).The concentrations of MCC were lower(P<0.01). Conclusion ROCK inhibitor fasudil, ameliorates myocardial fibrosis in diabetic rats at least in part by inhibiting oxidative stress and up-regulating eNOS expression.
    Expression of PBDC1 protein and preparation of its polyclonal antibody
    2014, 34(2):  222-225. 
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    Objective To express and purify PBDC1 protein by prokaryotic expression system, and to prepare polyclonal antibody. Methods The gene coding sequence of PBDC1 was inserted into pET-28a(+) vector to generate the pET-28a(+)-PBDC1 recombinant plasmid. Then PBDC1 protein was expressed in E.coli BL21(DE3) by IPTG induction and detected by SDS-PAGE. The fusion protein was purified by Ni2+ affinity chromatography. New Zealand rabbits were immuized with PBDC1 protein and the antiserum was obtained. Results Highly purified PBDC1 protein was obtained and identified by mass-spectrum. Western blot verified that the polyclonal antibody can specifically recognize PBDC1 protein. Conclusions The polyclonal antibody against PBDC1 is successfully prepared, which provides an efficient reagent for futher study of its role in erythroid differentiation.
    Clinical observation of paclitaxel chemotherapy by premedication procedure adjusted
    2014, 34(2):  226-228. 
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    Objective To observe the drug safety and adverse reactions of the different paclitaxel premedication, explore a new paclitaxel premedication procedure Methods Two kinds of the premedication procedure be used before injection of paclitaxel, one in standard premedication procedure, the other in adjusted premedication procedure, observe the drug safety and adverse reactions of the different paclitaxel premedication procedure, then explore a new paclitaxel premedication procedure Results The acute allergic reaction, neurotoxicity, gastrointestinal reaction rates were not statistically significant between 77 cases in application of adjustment premedication procedure and 78 cases in standard premedication procedure Conclusion The adjusted paclitaxel premedication procedure is safety, effective.
    A novel computer based method for the selection of genetic loci with single nucleotide polymorphism
    2014, 34(2):  229-234. 
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    Objective In this paper, a computer based method that could select the genetic loci with single nucleotide polymorphism (SNP) from amount of gene data with relative low costs and simple operations is proposed. Methods The algorithm with optimal risk and preventive pattern is applied to analyse the mutation of gene based on SNP loci after the extraction of feature property. The evolution of HBV (Hepatitis B Virus) is studied as an example to discover the phenomena of mutation during the transition from Acute to Chronic carrier, then to Cirrhosis. Results The computing experiment releases 18 potential SNPs locus that demonstrate the transition from Acute to Chronic, five of them have been reported by previous research, the remaining 13 are potential locus. In addition, 3 potential SNPs locus that demonstrate the transition to Cirrhosis are uncovered. Conclusion The proposed method can be used to select SNP locus from large number of gene data effectively and inexpensively, which may possibly be applied to the clinic diagnosis and basic research of HBV or other virus caused disease.
    Retro-virus mediated human p27kip1 over expression in HepG2 induced cell apoptosis
    2014, 34(2):  235-239. 
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    Objective To study the effect of retro-virus mediated human P27kip1 over expression in HepG2 cells. Method pLNCX vector containing human p27kip1 gene was constructed, and transfected into PA317 package cells, retrovirus containing P27kip1 was collected and infected HepG2 cells, over express P27kip1 cells was selected and the effect on HepG2 was determined. Result high efficient retrovirus vector was constructed, the P27kip1 gene was transferred into HepG2 cells and over expressed in HepG2 cells, and cells over express P72kip1 were caped in G1 phase, and cell growth was inhibited. Conclusion retrovirus vector pLNCX-P27 can efficiently import P27kip1 gene into HepG2 cells, over express P27kip1 inhibited HepG2 cell growth and induce apoptosis.
    A retrospective clinical study of clinically silent pheochromocytoma in 29 cases
    Zhi-en ZHOU, LI Han-zhong Bing-bing SHI
    2014, 34(2):  240-243. 
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    Objective To retrospectively analyze the clinical data of clinically silent pheochromocytoma in 29 cases in order to improve its diagnosis and treatment. Methods 29 patients were diagnosed pathologically as adrenal pheochromocytoma from May 2010 to May 2013, who were considered clinically silent and operated in Peking Union Medical College Hospital. We summarized the aspects include patient age, tumor location and maximal diameter, operation method, change of intraoperative blood pressure(BP), urinary catecholamine and the nuclear medicine examinations. Results Each of the 29 patients had a normal BP, and the results of urinary catecholamine were normal or elevated lightly. The maximal diameter of tumors were 1.5~14cm. 1 case was located in both side, while 13 cases in the right and 15 cases in the left. 25 were operated laparoscopically, and the other 4 underwent open operations. BP was stable in all the 23 cases who were given cardula, while 2 of 6 cases who were not given cardula had hypertensive crisis. 11/23 cases had false negative results in somatostatin receptor imaging, while 1/11 cases had a false negative result in 131IMIBG imaging. Conclusions Urinary catecholamine and somatostatin receptor imaging are not sensitive to clinically silent pheochromocytoma. 131IMIBG imaging is necessary to the cases who are highly suspicious in CT imaging. Surgical treatment should be carried out on the basis of correct drug preparation once the correct diagnosis is made.
    Interactions between inflammatory response and endoplasmic reticulum stress in atherosclerosis
    2014, 34(2):  253-256. 
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    Inflammatory response and endoplasmic reticulum stress (ERS) are significant events in the process of atherosclerosis (AS). Inflammatory response could induce ERS through several pathways, and ERS in turn may either enhance or reduce inflammatory response, according to what stage the atherosclerosis is in.
    The prospect of dedifferentiation for cell therapy
    2014, 34(2):  257-261. 
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    Dedifferentiation is an important life phenomenon in cell biology, which can be observed both in vivo and in vitro. The cells resulting from the dedifferentiation have the characteristics of undifferentiated cells. The process of dedifferentiation involving cell autophagy, recirculation of cells structure and a series of biological behavior is complex. More and more studies demonstrate the close relation between cell dedifferentiation and cell renewal, tissue regeneration and tumorigenesis, etc.
    Relationship between YAP protein and tumor
    2014, 34(2):  262-265. 
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    The Hippo-yap pathway is a newly growth regulatory pathway and regulate both cell growth and apoptosis, aberrant signaling pathway contribute to tumorigenesis. YAP protein is also involved in the mediate AKT-mTORC, WNT and MAPK signaling pathways, thereby affecting tumor cell growth, invasion and metastasis. YAP protein expression is increased in many types of human tumors, perhaps to become new therapeutic targets of tumor.
    The current research status of Endothelial progenitor cells and limb ischemia diseases
    2014, 34(2):  266-269. 
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    Limb ischemic disease has become one of the major diseases of developed countries as well as many developing countries, and stem cell transplantation is a new method for treatment in limb ischemic diseases ,it has attracted much attention. Endothelial progenitor cells (EPCs) can differentiate into endothelial cells involved in angiogenesis. Many studies have confirmed that the transplanted EPCs in the body can promote angiogenesis in ischemic tissue.
    Advances of HSP90 inhibitors in treating acute myelogenous leukemia
    2014, 34(2):  270-273. 
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    Heat shock protein 90 (HSP90) is a molecular chaperone with many oncogenic client which integrates multiple oncogenic pathways, involving the occurrence and poor prognosis in acute myelogenous leukemia (AML).Currently, HSP90 inhibitors are one research drugs of molecular targeted therapies in treating AML, via HSP90 inhibition to realize the regulation of multiple oncogenic pathways at the same time, and play a role of anti-leukemia effect.
    Roles of apolipoprotein A5 in regulation of triglyceride metabolism
    2014, 34(2):  274-277. 
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    Apolipoprotein A5 adjusting triglyceride metabolism possible mechanisms are: 1) directly or indirectly, activating lipoprotein lipase, accelerated lipolysis rich in triglycerides; 2) inhibit the synthesis and secretion of very low density lipoprotein; 3)speed up the liver for lipoprotein remnants of grain intake. Further clarify the role of apolipoprotein A5 in regulating metabolism of triglyceride, keen cerebrovascular disease for clinical diagnosis and treatment to provide new ideas.
    Improvement of type 2 diabetes by Biliopancreatic Diversion surgery
    2014, 34(2):  278-281. 
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    Bile and pancreatic juice were flowed straight into ileum after Biliopancreatic Diversion surgery,abating. digestive absorption because of that Bile and pancreatic juice transitorily mixed the food.The finding correlates with clinical observations of Biliopancreatic Diversion,which show that the surgeriy may improve effectingly the type 2 diabetes and its complication in a long tine, Having similar effects of gastric bypass surgery,but the mechanism is currently questionable,which may relate with gut hormone, blood fat,the function of islet cell and biliopancreatic diversion.
    A discussion on the rehabilitation mode of Taixing leprosy village
    2014, 34(2):  282-285. 
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    The leprosy village, a product of the special historical period, has made prominent contributions to the prevention and cure of leprosy. With the development of prevention and treatment, the number of the new-onset leprosy patients has decreased remarkably, so the rehabilitation therapy of the convalescents becomes the key work of leprosy village. In the summer of 2012, 28 studets at Grade 2008 of Department of Clinical Medicine, Peking Union Medical College, carried out a social practice in Taixing leprosy village, Jiangsu Province. In the survey, it has been found out that the rehabilitation mode of leprosy village in which all patients are insulated from the rest of society does have some drawbacks, the worst of which is the physical and psychological health of the convalescents couldn’t develop in a healthy way. Therefore, improving the rehabilitation mode and enhancing social humanistic concern should be a new developmental direction of the leprosy village.
    The application of internet in surgical education
    2014, 34(2):  286-288. 
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    To analyze the influence of internet on surgical education in the internet era, in order to take full advantage of the surgical education resource in the internet and improve the quality of surgical education.