Basic & Clinical Medicine ›› 2014, Vol. 34 ›› Issue (2): 190-195.

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The influence of of PTEN on VEGF and MMP expression in myeloid leukemia cells


  • Received:2013-06-09 Revised:2013-10-17 Online:2014-02-05 Published:2014-01-13
  • Contact: CHENG Zhi Yong

Abstract: Objective To investigate the influence and relationship of tumor-suppressing gene PTEN (phosphatase and tensin hemology deleted on chromosome ten gene), VEGF(vascular endothelial growth factor) and MMP (matrix metalloproteinase) in myeloid leukemia. Methods 1)To explore the PTEN, VEGF, MMP-2 and MMP-9 mRNA expression levels on 10 chronic myelogenous leukemia patients in chronic phase (CML-CP), 10 CML patients in blast crises (CML-BC), 10 normal control marrow mononuclear cells (MMNC). 2)The recombinated adenovirus containing green fluorescent protein (GFP) and PTEN(Ad-PTEN-GFP)or empty vector (Ad-GFP)was transfected into human leukemia K562 cells. The growth inhibition ratio of K562 cells were observed by MTT assay; PTEN, VEGF, MMP-2 and MMP9 mRNA levels were detected by real-time fluorescent relative-quantification reverse transcriptional PCR (FQ-PCR). PTEN, VEGF and p-Akt protein levels were detected by western blot, MMP-2 and MMP-9 protein were detected by gelatin zymogram. Results: The mRNA expression levels of PTEN in CML-BC patients were lower than CML-CP patients(P<0.05). But VEGF, MMP-2 and MMP-9 mRNA levels were reverse compared with PTEN(P<0.05). VEGF, MMP-2 and MMP-9 mRNA expression levels were down regulated about 4.80、5.88、5.72 fold; p-Akt, VEGF, MMP-2 and MMP-9 proteins were also down-regulated 26.0, 5.23, 2.86, 4.76 fold compared with Ad-GFP group; PTEN mRNA expression levels were negative with VEGF, MMP-2, MMP-9 mRNA after K562 cells transfected with wild type PTEN of MOI=200 after three days. Conclusion PTEN might have anti-angiogenesis and anti-adhesion ability in myeloid leukemia via inhibition VEGF, MMP-2 and MMP-9 expression.