CircRERE promotes proliferation, migration and invasion of human multiple myeloma cell lines by regulating miR-128-3p/WEE1 axis

doi:10.16352/j.issn.1001-6325.2024.02.0210

Abstract

Abstract: Objective To investigate the impacts of circular RNA RERE (circRERE) on proliferation, migration and invasion of human multiple myeloma (MM) cell lines by regulating microRNA (miR)-128-3p/serine/threonine protein kinase (WEE1) axis. Methods Normal plasma cells (nPCs) isolated from peripheral blood of healthy subjects and human MM cell lines(U266, RPMI-8226, NCI-H929, LP-1) were cultured. The expressions of circRERE and miR-128-3p were detected by RT-qPCR. The expression of WEE1 was detected by Western blot. After transfection, RPMI-8226 cells were divided into control group (without transfection), sh-circRERE group, sh-NC group, miR-128-3p inhibitor group, inhibitor-NC group, sh-circRERE+inhibitor-NC group and sh-circRERE+miR-128-3p inhibitor group. MTT assay and 5-ethynyl-2'-deoxyuridine (EdU) immunofluorescence staining microscopy were used to detect the proliferation. Migration and invasion were examined by scratch healing experiment as well as Transwell chamber assay. The targeting relationship between miR-128-3p and circRERE or WEE1 was verified though dual-luciferase reporter gene assay. Results Compared with nPCs, the expressions of circRERE and WEE1 in MM cells (RPMI-8226, U266, NCI-H929, LP-1) were increased, and the expression of miR-128-3p was decreased (P＜0.05). Compared with the control group, the proliferation rate, proportion of EdU positive cells, the healing rate of the scratch trauma and the number of invasion of the RPMI-8226 cells in sh-circRERE group were decreased(P＜0.05). However,miR-128-3p inhibitor group showed an opposite result and the difference was statistically significant (P＜0.05). miR-128-3p inhibitor significantly inhibited the effects of sh-circRERE on the proliferation, migration and invasion of RPMI-8226 cells (P＜0.05). Dual luciferase reporter gene experiments showed that circRERE/miR-128-3p and miR-128-3p/WEE1 had a targeting relationship. Conclusions CircRERE may promote proliferation, migration and invasion of RPMI-8226 cells, potentially with a mechanism of regulating miR-128-3p/WEE1 axis.

Key words: circRERE, miR-128-3p/WEE1 axis, multiple myeloma cells, proliferation, migration

CLC Number:

R733.3

FANG Yuan, LI Yi, WANG Wei. CircRERE promotes proliferation, migration and invasion of human multiple myeloma cell lines by regulating miR-128-3p/WEE1 axis[J]. Basic & Clinical Medicine, 2024, 44(2): 210-218.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/10.16352/j.issn.1001-6325.2024.02.0210

http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2024/V44/I2/210

References

[1]Cowan AJ, Green DJ, Kwok M, et al. Diagnosis and management of multiple myeloma: a review[J]. JAMA, 2022, 327: 464-477.
[2]张永梅,张志敏,周洁,等. LncRNA CBR3-AS1在多发性骨髓瘤中的表达及临床意义[J].基础医学与临床,2023,43:948-952.
[3]Fang W, Mu J, Yang Y, et al. CircRERE confers the resistance of multiple myeloma to bortezomib depending on the regulation of CD47 by exerting the sponge effect on miR-152-3p[J]. J Bone Oncol, 2021, 30: 100381. doi: 10.1016/j.jbo.2021.100381
[4]Zhou F, Wang D, Wei W, et al. Comprehensive profiling of circular RNA expressions reveals potential diagnostic and prognostic biomarkers in multiple myeloma[J]. BMC Cancer, 2020, 20: 40. doi: 10.1186/s12885-020-6515-2.
[5]Liu Q, Ran R, Song M, et al. LncRNA HCP5 acts as a miR-128-3p sponge to promote the progression of multiple myeloma through activating Wnt/β-catenin/cyclin D1 signaling via PLAGL2[J]. Cell Biol Toxicol, 2022, 38: 979-993.
[6]Ghelli Luserna di Rorà A, Cerchione C, Martinelli G, et al. A WEE1 family business: regulation of mitosis, cancer progression, and therapeutic target[J]. J Hematol Oncol, 2020, 13: 126-142.
[7]Ling Z, Zhang J, Liu Q. Oncogenic Forkhead box D3 antisense RNA 1 promotes cell survival and confers temozolomide resistance in glioblastoma cells through the miR-128-3p/WEE1 G2 checkpoint kinase axis[J]. Bioengineered, 2022, 13: 6012-6023.
[8]Jakobsen T, Dahl M, Dimopoulos K, et al. Genome-wide circular RNA expression patterns reflect resistance to immunomodulatory drugs in multiple myeloma cells[J]. Cancers (Basel), 2021, 13: 365-383.
[9]Chen D, Yang X, Liu M, et al. Roles of miRNA dysregulation in the pathogenesis of multiple myeloma[J]. Cancer Gene Ther, 2021, 28: 1256-1268.
[10]杨芒庄,杨旭东,王晓龙. Circ-MALAT1靶向miR-101抑制膀胱癌细胞系SW780侵袭和迁移[J]. 基础医学与临床,2022,42:472-478.
[11]Feng Y, Zhang L, Wu J, et al. CircRNA circ_0000190 inhibits the progression of multiple myeloma through modulating miR-767-5p/MAPK4 pathway[J]. J Exp Clin Cancer Res, 2019, 38: 54-70.
[12]Wang Y, Lin Q, Song C, et al. Depletion of circ_0007841 inhibits multiple myeloma development and BTZ resistance via miR-129-5p/JAG1 axis[J]. Cell Cycle, 2020, 19:3289-3302.
[13]Gorecki L, Andrs M, Korabecny J. Clinical candidates targeting the ATR-CHK1-WEE1 axis in cancer[J]. Cancers (Basel), 2021, 13: 795-816.
[14]华科雷,任莹坤,霍明科,等. miR-128-3p在胃癌组织中的表达及其对胃癌细胞迁移和侵袭能力的影响[J]. 中华普通外科杂志,2022,37:279-283.

[1]	LIAO Zhencheng, YANG Siyi, WU Ping'an. Increased expression of m⁶A demethylase FTO inhibits the proliferation of nasopharyngeal carcinoma cells [J]. Basic & Clinical Medicine, 2024, 44(1): 57-62.
[2]	LI Jiangli, SUN Jing, TANG Yijun, GUO Junlan, CHEN Bo, GUO Shengnan. Circ-DDX5 inhibits the proliferation and invasion of human breast cancer cell lines by targeting miR-3940 [J]. Basic & Clinical Medicine, 2024, 44(1): 1-7.
[3]	PAN Shan, LIN Kengqiang, CHEN Shuxing. Pemetrexed inhibits the proliferation of lung adenocarcinoma cell line A549 [J]. Basic & Clinical Medicine, 2023, 43(9): 1359-1363.
[4]	LIANG Huiyong, WEI Qiuhui, DING Ya, PENG Xiaoqing, YAO Jing, SU Qijian. Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α [J]. Basic & Clinical Medicine, 2023, 43(9): 1390-1393.
[5]	YANG Tingting, HUANG Jie, GUO Jianxin, HAN Jian. DEPDC1 is up-regulated in uterine corpus endometrial cancer [J]. Basic & Clinical Medicine, 2023, 43(9): 1399-1407.
[6]	HUANG Jie, LI Runbo, GUO Jianxin, HAN Jian. Expression of DDX5 and DDX17 in ovarian cancer tissues and their effects on proliferation and drug resistance of human ovarian cancer cell line SKOV3 [J]. Basic & Clinical Medicine, 2023, 43(8): 1247-1253.
[7]	ZHANG Peibo, LI Yi. Total flavonoids of Hippophae rhamnoides L. inhibits proliferation of bladder cancer cell line T24 [J]. Basic & Clinical Medicine, 2023, 43(7): 1122-1126.
[8]	TANG Yijun, WANG Wenlong, LI Jiangli, YANG Huan, SUN Jing. LncRNA RP11-686D22.10 is related with the survival of breast cancer patients, affects the proliferation, invasion of breast cancer cell lines [J]. Basic & Clinical Medicine, 2023, 43(7): 1023-1029.
[9]	JIN Xiaoxia, LU Xiaoyun, LIU Yushan, LIANG Lina. Expression and clinical significance of SIRT2 in urothelial bladder cancer and its impact on cancer cell proliferation, invasion and migration [J]. Basic & Clinical Medicine, 2023, 43(7): 1090-1096.
[10]	ZENG Yankun, HONG Xiaoying, CHEN Qinglin. Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1 [J]. Basic & Clinical Medicine, 2023, 43(7): 1104-1109.
[11]	SU Jingyu, ZHANG Zhenxi, DU Wenjing. Effect of TPX2 expression on prognosis of pancreatic carcinoma [J]. Basic & Clinical Medicine, 2023, 43(6): 875-881.
[12]	ZHANG Hailing, YANG Yu, ZHOU Qianqian, WANG Yuanjin, WANG Ting. Neutrophil NETs treated with microcystin-LR promote migration of colorectal cancer cell [J]. Basic & Clinical Medicine, 2023, 43(6): 967-973.
[13]	HOU Mengjie, ZHAO Na, HUANG Fuqiang, WANG Yanan, LIU Fangming. Apolipoprotein E negatively regulates Golgi membrane protein 73 to inhibit the proliferation of mouse liver cancer cell lines [J]. Basic & Clinical Medicine, 2023, 43(5): 762-770.
[14]	LIU Yue, CAO Yang, GU Weiying, SHANG Limei, LIU Yan. Decitabine combined with arsenic trioxide inhibits proliferation and promotes apoptosis of acute myeloid leukemia cell lines [J]. Basic & Clinical Medicine, 2023, 43(4): 608-614.
[15]	WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang. Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480 [J]. Basic & Clinical Medicine, 2023, 43(2): 241-245.