SDF-1/CXCR4轴活化诱导内皮祖细胞增殖、迁移及管型形成

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (5): 428-431.

SDF-1/CXCR4轴活化诱导内皮祖细胞增殖、迁移及管型形成

陈飞兰张华蓉徐承平卞修武

第三军医大学西南医院病理科第三军医大学西南医院病理科第三军医大学西南医院病理科第三军医大学西南医院病理科

收稿日期:2007-11-02 修回日期:2007-12-25 出版日期:2008-05-25 发布日期:2008-05-25
通讯作者: 卞修武

Activation of SDF-1/CXCR4 Axis Induces Proliferation, Migration, and Tubulogenesis of Human Endothelial Progenitor Cells

Fei-lan CHEN, Hua-rong ZHANG, Cheng-ping XU, Xiu-wu BIAN

Institute of Pathology, Southwest Hospital, the Third Military Medical University Institute of Pathology, Southwest Hospital, the Third Military Medical University

Received:2007-11-02 Revised:2007-12-25 Online:2008-05-25 Published:2008-05-25
Contact: Xiu-wu BIAN

摘要/Abstract

摘要： 目的观察趋化因子SDF-1促内皮祖细胞增殖、迁移和管型形成的作用。方法用免疫细胞化学检测内皮祖细胞SDF-1和CXCR4表达；用MTT法、Millicell趋化法及Matrigel体外三维成型法分别检测不同浓度的趋化因子SDF-1促内皮祖细胞增殖、迁移和管型形成。并应用CXCR4受体抑制剂AMD3100观察上述指标的变化。结果免疫细胞化学显示内皮祖细胞表达SDF-1和CXCR4蛋白。SDF-1可促进内皮祖细胞的增殖、迁移和体外小管样结构的形成。AMD3100可抑制SDF-1的诱导作用。结论SDF-1/CXCR4轴在内皮祖细胞参与血管新生中可能发挥重要作用。

关键词: 内皮祖细胞, 趋化因子受体CXCR4, 血管新生化

Abstract: objective To explore the effect of chemokine SDF-1 on the proliferation, migration and in vitro of human endothelial progenitor cells (EPCs). Methods The expression of CXCR4 and SDF-1 on EPCs was detected with immunocytochemistry. Proliferation, migration and in vitro tubulogenesis of EPCs were detected by MTT, Millicell chemotaxis and three-diamensional in vitro Matrigel assays, respectively. When activation or inactivation of SDF-1/CXCR4 axis with CXCR4 ligand SDF-1 or CXCR4 inhibitor AMD3100. Results SDF-1 and CXCR4 were expressed on EPCs. SDF-1 induced proliferation, migration and tubulogenesis of EPCs. However, AMD3100 abolished the effects induced by SDF-1. Conclusion The SDF-1/CXCR4 axis possibly plays a critical role in regulating vasculogenesis of EPCs

Key words: EPCs, CXCR4, neovascularization

陈飞兰张华蓉徐承平卞修武. SDF-1/CXCR4轴活化诱导内皮祖细胞增殖、迁移及管型形成[J]. 基础医学与临床, 2008, 28(5): 428-431.

Fei-lan CHEN; Hua-rong ZHANG; Cheng-ping XU; Xiu-wu BIAN. Activation of SDF-1/CXCR4 Axis Induces Proliferation, Migration, and Tubulogenesis of Human Endothelial Progenitor Cells[J]. Basic & Clinical Medicine, 2008, 28(5): 428-431.

[1]	李伟麒郑磊王前苏亚娟蔡贞. 外周血内皮祖细胞的诱导培养[J]. 基础医学与临床, 2010, 30(7): 743-748.
[2]	王琴马可仉红刚张秋菊修瑞娟. 内皮祖细胞的体外培养[J]. 基础医学与临床, 2010, 30(6): 603-608.
[3]	刘春喜李大庆张运姜虹王荣王旭平. 兔外周血内皮祖细胞培养及tHK 基因体外修饰的研究[J]. 基础医学与临床, 2007, 27(11): 1241-1245.