基础医学与临床 ›› 2022, Vol. 42 ›› Issue (1): 62-67.

• 研究论文 • 上一篇    下一篇

蛇菰多糖降低实验性肝损伤大鼠肝脏BDNF和TrkB的表达

谢雅1,陈颖1,夏德尧1,唐鲜娥1,王凤杰2,陈显兵1   

  1. 1. 湖北民族大学医学部
    2. 湖北民族学院医学院
  • 收稿日期:2020-11-27 修回日期:2021-05-05 出版日期:2022-01-05 发布日期:2022-01-05
  • 通讯作者: 陈显兵 E-mail:653388390@qq.com
  • 基金资助:
    国家自然科学基金项目;湖北民族大学青年科研基金项目;湖北省教育厅科学研究计划项目;恩施州科技局项目

Balanophora polysaccharide decreases the expressions of BDNF and TrkB in liver tissues of rat with experimental liver injury

  • Received:2020-11-27 Revised:2021-05-05 Online:2022-01-05 Published:2022-01-05
  • Contact: chen xianbing E-mail:653388390@qq.com

摘要: 目的 蛇菰多糖(BPS)对D-gal所致实验性肝损伤大鼠的肝功能及肝组织内BDNF和TrkB蛋白、凋亡相关蛋白表达的影响。方法 将大鼠随机分为,对照组(control),D-gal组(Model,皮下注射D-半乳糖200 mg/kg),BPS低(BPS-L)、中(BPS-M)、高(BPS-H)剂量组,分别每天灌胃50、100和200 mg/kg BPS。共6周,心脏取血检测血清ALT和AST水平;HE染色法观察肝组织形态;免疫组织化学染色检测BDNF和TrkB的定位;Western blot检测BDNF、TrkB、Bcl-2、caspase3和Bax蛋白表达;ELISA测定肝组织内MDA含量和SOD活性。结果 与对照组相比,D-gal组肝细胞水肿、点状坏死;血清ALT和AST水平升高(P<0.05);SOD活性降低、MDA含量升高(P<0.05);Bax、caspase-3、BDNF、TrkB表达增加(P<0.05),Bcl-2表达降低(P<0.05);与D-gal组相比,BPS各剂量组肝细胞变性、坏死明显减轻;血清ALT和AST水平降低(P<0.05);SOD活性升高、MDA含量降低(P<0.05);促凋亡蛋白Bax和caspase-3表达降低,抗凋亡蛋白Bcl-2表达增加(P<0.05);BDNF 和TrkB的表达降低(P<0.05)。结论 蛇菰多糖对D-gal所致实验性肝损伤有一定的保护作用,其机制可能与降低肝组织内BDNF和TrkB的表达、减轻肝细胞凋亡有关。

关键词: 蛇菰多糖, 肝损伤, 凋亡, BDNF/TrkB

Abstract: Objective To study the effects of Balanophora polysaccharides (BPS) on the expression of BDNF and TrkB proteins and apoptosis-related proteins in rats with experimental liver injury induced by D-gal. Methods Rats were randomly divided into control group (control), D-gal (model, 200 mg /kg of hypodermic D- galactose), BPS low (BPS-L), medium (BPS-M), high (BPS-H) dose groups, which were treated with intragastric administration by 50, 100 and 200 mg/kg BPS per day, respectively. For six weeks in total, serum ALT and AST levels were measured by cardiac blood collection. HE staining was used to observe liver tissue morphology. The localization of BDNF and TrkB was determined by immunohistochemical staining. Western Blot was used to detect BDNF, TrkB, Bcl-2, Caspase-3 and Bax, protein expression. ELISA was used to determine the MDA content and SOD activity in liver tissues. Results Compared with the control group, the hepatocytes had edema and punctate necrosis in D-gal group, with increased serum ALT and AST levels (P <0.05), decreased SOD activity and increased MDA content (P <0.05), increased Bax, Caspase-3, BDNF, TrkB expression (P <0.05), decreased Bcl-2 expression (P <0.05). Compared to D-gal group, the hepatocyte degeneration and necrosis were obviously alleviated in BPS groups with each dose, with decreased serum ALT and AST levels (P <0.05), increased SOD activity and decreased MDA content (P <0.05), reduced B ax and Caspase-3 expression of pro-apoptotic proteins, increased expression Bcl-2 anti-apoptotic proteins (P <0.05), and decreased expression of BDNF and TrkB (P <0.05). Conclusions Balanophora polysaccharide had aprotective effect on experimental liver injury caused by D-gal. The mechanism may be related to the decrease of the expression of BDNF and TrkB and the decrease of hepatocyte apoptosis.

Key words: Balanophora polysaccharide, Liver injury, Apoptosis, BDNF/TrkB