miR-29b抑制LPS诱导的人支气管上皮细胞系16HBE的凋亡

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (3): 333-339.

miR-29b抑制LPS诱导的人支气管上皮细胞系16HBE的凋亡

袁东^1,3, 蒋瑶娜^2,3, 李亚清^3*

1.蚌埠医学院研究生院, 安徽蚌埠 233030;
2.浙江中医药大学第二临床医学院, 浙江杭州 310053;
3.浙江省人民医院呼吸内科, 浙江杭州 310014

收稿日期:2020-07-06 修回日期:2020-10-19 出版日期:2021-03-05 发布日期:2021-03-01
通讯作者: ^*lidoctor03@126.com
基金资助:
国家自然科学基金(8187010018)

miR-29b inhibits LPS-induced apoptosis in human bronchial epithelial cell line 16HBE

YUAN Dong^1,3, JIANG Yao-na^2,3, LI Ya-qing^3*

1. Graduate School of Bengbu Medical College, Bengbu 233030;
2. the Second Clinical Medical College of Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053;
3. Department of Respiratory Medicine, Zhejiang Provincial People's Hospital, Hangzhou 310014, China

Received:2020-07-06 Revised:2020-10-19 Online:2021-03-05 Published:2021-03-01
Contact: ^*lidoctor03@126.com

摘要/Abstract

摘要： 目的研究miR-29b对脂多糖(LPS)诱导的人支气管上皮细胞系16HBE凋亡的影响。方法将16HBE细胞分为对照组、LPS组(含50 μg/mL LPS的细胞培养液培养)、转染mimics control组和转染miR-29b mimics组。MTT法检测增殖;流式细胞测量术检测凋亡;Western blot检测c-caspase-3、c-caspase-12、糖调节蛋白78(GRP78)和CCAAT/增强子结合蛋白同源蛋白(CHOP)蛋白表达。在16HBE细胞中共转染miR-29b mimics、pcDNA-CHOP,同样利用上述方法检测增殖、凋亡。结果与对照组比较,LPS组细胞增殖活性降低、凋亡率升高(P＜0.05),细胞中c-caspase-3、c-caspase-12、GRP78和CHOP蛋白表达水平升高(P＜0.05)。与转染mimics control组比较,转染miR-29b mimics组细胞增殖活性升高、凋亡率降低(P＜0.05),细胞中c-caspase-3、c-caspase-12、GRP78和CHOP蛋白表达水平降低(P＜0.05)。pcDNA-CHOP可以逆转miR-29b mimics对LPS条件下支气管上皮细胞增殖和凋亡的影响。结论 miR-29b抑制LPS诱导的16HBE细胞的凋亡,其作用机制与抑制内质网应激有关。

关键词: miR-29b, 支气管上皮细胞, 脂多糖, 凋亡

Abstract: Objective To study the effect of miR-29b on lipopolysaccharide(LPS)-induced apoptosis of human bronchial epithelial cell line 16HBE. Methods 16HBE cells were divided into control group, LPS group (LPS treatment), transfection mimics control group, transfection miR-29b mimics group. MTT assay for proliferation, flow cytometry was used for apoptosis; Western blot was used for checking the expression of cleaved cysteinyl aspartate specific proteins 3(c-caspase-3), cleaved cysteinyl aspartate specific proteinase 12(c-caspase-12), glucose-regulated protein 78(GRP78) and CCAAT/enhancer-binding protein C/EBP (CHOP) protein. Human bronchial epithelial cells 16HBE were co-transfected with miR-29b mimics and pcDNA-CHOP, the proliferation and apoptosis were also determined using the above method. Results Compared with the control group, the cell proliferation- activity and apoptosis rate of the LPS group decreased, and the protein expression level of c-caspase-3, c-caspase-12, GRP78, and CHOP all increased. Compared with the LPS+NC group, the LPS+miR-29b group showed an increased cell proliferation, decreased apoptosis and decreased expression of c-caspase-3, c-caspase-12, GRP78, and CHOP protein. pcDNA-CHOP reversed the effects of miR-29b mimics on the proliferation and apoptosis of bronchial epithelial cells under LPS conditions. Conclusions miR-29b inhibits 16HBE apoptosis induced by LPS; the mechanism might be related to the inhibition of endoplasmic reticulum stress.

Key words: miR-29b, bronchial epithelial cells, lipopolysaccharide, apoptosis

中图分类号:

R562.2

袁东, 蒋瑶娜, 李亚清. miR-29b抑制LPS诱导的人支气管上皮细胞系16HBE的凋亡[J]. 基础医学与临床, 2021, 41(3): 333-339.

YUAN Dong, JIANG Yao-na, LI Ya-qing. miR-29b inhibits LPS-induced apoptosis in human bronchial epithelial cell line 16HBE[J]. Basic & Clinical Medicine, 2021, 41(3): 333-339.

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