基础医学与临床 ›› 2021, Vol. 41 ›› Issue (12): 1762-1766.

• 研究论文 • 上一篇    下一篇

lncRNA RAB11B-AS1上调miR-628-3p抑制人胃癌细胞系增殖和迁移

杨宁波, 贾晓东*, 寇耀   

  1. 遂宁市中心医院 胃肠外科,四川 遂宁 629000
  • 收稿日期:2020-08-28 修回日期:2021-01-09 发布日期:2021-12-03
  • 通讯作者: *176161804@qq.com
  • 基金资助:
    四川省卫生厅科研计划项目(130493)

lncRNA RAB11B-AS1 up-regulating miR-628-3p inhibits proliferation and migration of gastric cancer cell line

YANG Ning-bo, JIA Xiao-dong*, KOU Yao   

  1. Department of Gastrointestinal Surgery, Suining Central Hospital, Suining 629000, China
  • Received:2020-08-28 Revised:2021-01-09 Published:2021-12-03
  • Contact: *176161804@qq.com

摘要: 目的 探讨lncRNA RAB11B-AS1对胃癌细胞增殖、迁移、侵袭和顺铂敏感性的影响及分子机制。方法 建立顺铂耐药胃癌细胞系(HGC-27/DDP),将其分为对照组、si-NC组和si-RAB11B-AS1组;四甲基偶氮唑盐比色法(MTT)检测细胞存活率;实时荧光定量PCR(RT-qPCR)检测RAB11B-AS1和miR-628-3p的表达;流式细胞仪检测细胞周期;Transwell小室法检测细胞迁移和侵袭;双荧光素酶报告实验检测RAB11B-AS1和miR-628-3p的靶向关系。结果 0.2、0.4 mg/L顺铂处理后HGC-27细胞存活率显著降低(P<0.05),而0~0.4 mg/L的顺铂处理后HGC-27/DDP细胞存活率无统计学意义。HGC-27和HGC-27/DDP细胞中RAB11B-AS1表达水平显著升高(P<0.05),且HGC-27/DDP细胞中RAB11B-AS1表达水平显著高于HGC-27细胞(P<0.05)。lncRNA RAB11B-AS1干扰表达,G0-G1期细胞所占比例显著升高,S期细胞所占比例显著降低(P<0.05);细胞存活率显著降低,迁移、侵袭细胞数显著降低(P<0.05)。RAB11B-AS1靶向调控miR-628-3p。结论 LncRNA RAB11B-AS1可通过上调miR-628-3p表达抑制胃癌细胞增殖、迁移、侵袭,并提高癌细胞对顺铂的敏感性。

关键词: ncRNA RAB11B-AS1, miR-628-3p, 胃癌, 增殖, 顺铂, 敏感性

Abstract: Objective To explore the effect and molecular mechanism of lncRNA RAB11B-AS1 on gastric cancer cell proliferation, migration, invasion and cisplatin sensitivity. Methods The cisplatin-resistant gastric cancer cells (HGC-27/DDP) were divided into control group, si-NC group, and si-RAB11B-AS1 group; tetra-methylazolium salt colorimetric assay(MTT) was used to detect cell survival rate; real-time fluorescence quantitative PCR (RT-qPCR) was applied to detect the expressions of RAB11B-AS1 and miR-628-3p; flow cytometry was used to detect cell cycle; cell migration and invasion were examined by Transwell cell method and dual luciferase report experiment was applied to detect the targeting relationship between RAB11B-AS1 and miR-628-3p. Results The survival rate of HGC-27 cells was significantly reduced after 0.2 and 0.4 mg/L cisplatin treatment (P<0.05), but the survival rate of HGC-27/DDP cells after 0-0.4 mg/L cisplatin treatment was not statistically changed. The expression of RAB11B-AS1 in HGC-27 andHGC-27/DDP cells was significantly increased (P<0.05), and the expression of RAB11B-AS1 in HGC-27/DDP cells was significantly higher than that of HGC-27 cells (P<0.05). Interfering with the expression of lncRNA RAB11B-AS1, the proportion of G0-G1 phase cells was increased significantly, the proportion of S phase cells was decreased significantly (P<0.05). Both cell survival rate and the number of migrating and invasive cells were significantly reduced (P<0.05). RAB11B-AS1 was found to specifically regulate miR-628-3p. Conclusions LncRNA RAB11B-AS1 can inhibit the proliferation, migration and invasion of gastric cancer cells by up-regulating the expression of miR-628-3p, and increase the sensitivity of cancer cells to cisplatin.

Key words: lncRNA RAB11B-AS1, miR-628-3p, gastric cancer, proliferation, cisplatin, sensitivity

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