黄芪多糖抑制多发性骨髓瘤细胞系U266增殖、迁移和侵袭

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (8): 1083-1089.

黄芪多糖抑制多发性骨髓瘤细胞系U266增殖、迁移和侵袭

李清平¹, 刘会群², 蔡萼^2*

1.湖北省荆门市第二人民医院病理科, 湖北荆门 448000;
2.湖北省荆门市第二人民医院检验科, 湖北荆门 448000

收稿日期:2019-06-13 修回日期:2019-11-04 出版日期:2020-08-05 发布日期:2020-07-29
通讯作者: ^*caie0813@163.com
基金资助:
湖北省课题基金(EK2016D170025000665)

Astragalus polysaccharide inhibits proliferation, migration and invasion of multiple myeloma cell line U266

LI Qing-ping¹, LIU Hui-qun², CAI E^2*

1. Department of Pathology, Second People's Hospital of Jingmen City, Hubei Province, Jingmen 448000, China;
2. Department of Clinical Laboratory, Second People's Hospital of Jingmen City, Hubei Province, Jingmen 448000, China

Received:2019-06-13 Revised:2019-11-04 Online:2020-08-05 Published:2020-07-29
Contact: ^*caie0813@163.com

摘要/Abstract

摘要： 目的探讨黄芪多糖(APS)对多发性骨髓瘤(MM)细胞系U266增殖、凋亡、迁移和侵袭的影响及可能的作用机制。方法培养U266细胞,用不同浓度(6、 8和10 mg/mL)的APS作用U266细胞24、48和72 h,或8 mg/mL的APS作用于转染肿瘤坏死因子受体相关因子6(TRAF6)表达质粒(pcDNA3.1-TRAF6)的U266细胞48 h,CCK-8法检测U266细胞增殖;流式细胞仪检测U266细胞凋亡;Transwell检测U266细胞迁移和侵袭;Western blot检测cyclin D1、p21、Bax、Bcl-2、E-cadherin、MMP-2和TRAF6蛋白表达。结果与对照组相比,APS组U266细胞抑制率、凋亡率、迁移和侵袭细胞数均显著升高(P＜0.05),cyclin D1、Bcl-2、MMP-2和TRAF6蛋白表达水平显著降低(P＜0.05), p21、Bax、和E-cadherin蛋白表达水平显著升高(P＜0.05)。与APS+pcDNA3.1组比,APS+pcDNA3.1-TRAF6组U266细胞抑制率、凋亡率、迁移和侵袭细胞数均显著降低(P＜0.05),cyclin D1、Bcl-2、MMP-2和TRAF6蛋白表达水平显著升高(P＜0.05), p21、Bax、和E-cadherin蛋白表达水平显著降低(P＜0.05)。结论 APS可能通过下调TRAF6蛋白表达抑制U266细胞的增殖、迁移和侵袭,促进U266细胞凋亡。

关键词: 多发性骨髓瘤, APS, TRAF6, 凋亡, 迁移

Abstract: Objective To investigate the effects of astragalus polysaccharide (APS) on proliferation, apoptosis, migration and invasion of multiple myeloma (MM) cell line U266 and potential mechanisms. Methods U266 cells were incubated with APS at different concentrations (6, 8 and 10 mg/mL) for 24, 48 and 72 h, or 8 mg/mL treated APS on U266 cells transfected with tumor necrosis factor receptor-associated factor 6 (TRAF6) expression plasmid (pcDNA3.1-TRAF6) for 48 h.The proliferation of U266 cells was detected by CCK-8 method. Apoptosis of U266 cells was detected by flow cytometry. Migration and invasion of U266 cells were detected by Transwell. The level of cyclin D1, p21, Bax, Bcl-2, E-cadherin, MMP-2 and TRAF6 protein expression was detected by Western blot. Results Compared with the control, the inhibition rate, apoptosis rate, migration and invasion number of U266 cells in APS group were significantly increased(P＜0.05), and the expression of cyclin D1, Bcl-2, MMP-2 and TRAF6 was significantly decreased(P＜0.05), and the expression of p21, Bax and E-cadherin was significantly elevated(P＜0.05). Compared with the APS+pcDNA3.1 group, the inhibition rate, apoptosis rate, migration and invasion number of U266 cells in APS+pcDNA3.1-TRAF6 group were significantly decreased(P＜0.05), and the expression of cyclin D1, Bcl-2, MMP-2 and TRAF6 was significant elevated(P＜0.05). The expression of p21, Bax and E-cadherin was significantly inhibited(P＜0.05). Conclusions APS may inhibit the proliferation, migration and invasion of U266 cells through down-regulating the expression of TRAF6 protein, and promote the apoptosis of U266 cells.

Key words: multiple myeloma, astragalus polysaccharides, TRAF6, apoptosis, migration

中图分类号:

R733.3

李清平, 刘会群, 蔡萼. 黄芪多糖抑制多发性骨髓瘤细胞系U266增殖、迁移和侵袭[J]. 基础医学与临床, 2020, 40(8): 1083-1089.

LI Qing-ping, LIU Hui-qun, CAI E. Astragalus polysaccharide inhibits proliferation, migration and invasion of multiple myeloma cell line U266[J]. Basic & Clinical Medicine, 2020, 40(8): 1083-1089.

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