人参皂苷Rb1减轻人脐静脉内皮细胞氧化损伤

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (8): 1064-1067.

人参皂苷Rb1减轻人脐静脉内皮细胞氧化损伤

宋志明¹, 余舒杰², 焦洁¹, 李平平¹, 李红梅¹, 钱孝贤^2*

1.河南大学第一附属医院心内科, 河南开封 475001;
2.中山大学附属第三医院心内科,广东广州 510630

收稿日期:2020-03-16 修回日期:2020-05-25 出版日期:2020-08-05 发布日期:2020-07-29
通讯作者: ^*qianxx@mail.sysu.edu.cn
基金资助:
河南省医学科技攻关计划(SB201901065, 2018020307);河南省高等学校重点科研项目(19A320019);河南省科技发展计划(182102310559,202102310373);开封市科技计划(1903008,2003025)

Ginsenoside Rb1 alleviates oxidative damage of HUVECs

SONG Zhi-ming¹, YU Shu-jie², JIAO Jie¹, LI Ping-ping¹, LI Hong-mei¹, QIAN Xiao-xian^2*

1. Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng 475001;
2. Department of Cardiology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China

Received:2020-03-16 Revised:2020-05-25 Online:2020-08-05 Published:2020-07-29
Contact: ^*qianxx@mail.sysu.edu.cn

摘要/Abstract

摘要： 目的探讨人参皂苷Rb1(gRb1)对过氧化氢(H₂O₂)致人脐静脉内皮细胞(HUVECs)氧化损伤的影响。方法将HUVECs分为对照组、H₂O₂(20、40、80和160 μmol/L)组、gRb1(10、20和40 μmol/L)干预组。MTT法测定细胞存活率;annexin V-FITC/PI双染法检测凋亡;黄嘌呤氧化酶法测定SOD1活性;硫代巴比妥比色法计算MDA含量;Western blot测定细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)蛋白表达。结果与对照组相比,H₂O₂呈浓度依赖性抑制细胞存活率(P＜0.05),增加细胞凋亡(P＜0.05),抑制SOD1活性(P＜0.05),增加MDA含量(P＜0.05),促进ICAM-1和VCAM-1蛋白表达(P＜0.05)。gRb1干预能够显著缓解上述指标的变化。结论 gRb1通过抑制细胞凋亡、改善氧化应激水平和抑制ICAM-1及VCAM-1蛋白表达减轻HUVECs氧化损伤。

关键词: 人参皂苷Rb1, 氧化损伤, 细胞间黏附分子-1, 血管细胞黏附分子-1

Abstract: Objective To explore the effect of ginsenoside Rb1 (gRb1) on oxidative damage of human umbilical vein endothelial cells (HUVECs) resulted from hydrogen peroxide (H₂O₂). Methods HUVECs were divided into control group, H₂O₂ group and gRb1 group. MTT assay was used to determine cell survival; annexin V-FITC/PI double staining was used to detect cell apoptosis; SOD1 activity was measured with xanthine oxidase method; MDA concentration was detected by thibatituric acid assay; protein expression of ICAM-1 and VCAM-1 was analysed by Western blot. Results Compared with control group, the apoptosis rate, MDA production and the protein level of ICAM-1 and VCAM-1 significantly increased, but the cell viability and SOD1 activity decreased. Compared with H₂O₂ group, gRb1 reversed the parameters mentioned above. Conclusions gRb1 can alleviate oxidative damage of HUVECs by reducing cell apoptosis, oxidative stress and protein expression of ICAM-1 and VCAM-1.

Key words: ginsenoside Rb1, oxidative injury, ICAM-1, VCAM-1

中图分类号:

R339.3⁺8

宋志明, 余舒杰, 焦洁, 李平平, 李红梅, 钱孝贤. 人参皂苷Rb1减轻人脐静脉内皮细胞氧化损伤[J]. 基础医学与临床, 2020, 40(8): 1064-1067.

SONG Zhi-ming, YU Shu-jie, JIAO Jie, LI Ping-ping, LI Hong-mei, QIAN Xiao-xian. Ginsenoside Rb1 alleviates oxidative damage of HUVECs[J]. Basic & Clinical Medicine, 2020, 40(8): 1064-1067.

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