小热休克蛋白家族和自噬相关蛋白在自发性2型糖尿病db/db小鼠海马组织中的表达

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (8): 1059-1063.

小热休克蛋白家族和自噬相关蛋白在自发性2型糖尿病db/db小鼠海马组织中的表达

李金凤, 李小承, 殷小红, 马卫国, 李亚^*

西安医学院第一附属医院内分泌科,陕西西安 710075

收稿日期:2019-07-12 修回日期:2019-12-30 出版日期:2020-08-05 发布日期:2020-07-29
通讯作者: ^*yaliya19@163.com
基金资助:
陕西省教育厅自然科学基金(2013JK0781);西安市科技计划基金项目[201805094YX2SF28(12)];西安医学院第一附属医院基金(XYFY2015-01)

Expression of small heat shock protein family and autophagy related proteins in the hippocampus of db/db mice with spontaneous type 2 diabetes

LI Jin-feng, LI Xiao-cheng, YIN Xiao-hong, MA Wei-guo, LI Ya^*

(Department of Endocrindogy, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710075, China

Received:2019-07-12 Revised:2019-12-30 Online:2020-08-05 Published:2020-07-29
Contact: ^*yaliya19@163.com

摘要/Abstract

摘要： 目的观察自发性2型糖尿病db/db小鼠海马组织中小热休克蛋白家族(sHSPs)及自噬相关蛋白表达情况。方法正常db/m小鼠作为对照组,糖尿病db/db小鼠作为模型组,各10只。观察其体质量,空腹血糖(FBG);real-time PCR检测海马组织中小热休克蛋白家族、自噬相关基因mRNA的表达;Western blot检测HSPB8、BAG3、LC3、P62蛋白表达。结果 1)db/db小鼠体质量、空腹血糖均明显高于db/m小鼠(P＜0.01)。2)小热休克蛋白1-10(heat shock protein family B [small] member 1-10,HSPB 1-10)基因mRNA在小鼠海马组织中均有表达;其中HSPB1、HSPB3、HSPB5、HSPB6和HSPB8 db/db组明显低于db/m组(P＜0.05);HSPB2、HSPB9和HSPB10 db/db组高于db/m组(P＜0.05);而在db/db小鼠中LC3 表达高于db/m组(P＜0.01),而P62反之(P＜0.01)。3)较db/m组,db/db组HSPB8、BAG3、LC3-Ⅱ蛋白表达均增高(P＜0.01),相反P62蛋白表达减低(P＜0.05)。结论 1)10种sHSPs均在小鼠海马组织中表达,但表达特点有所不同。2)8周龄糖尿病小鼠海马组织中存在HSPB8蛋白表达增强,且自噬激活。

关键词: 糖尿病, 小热休克蛋白, 自噬, db/db小鼠

Abstract: Objective To study the changes of small heat shock proteins (sHSPs) and autophagy-related proteins in hippocampus of spontaneous type 2 diabetic db/db mice. Methods The db/m mice were used as control group, while diabetic db/db mice were used as model group, 10 mice in each group. The body weight, fasting blood glucose (FBG), the expression of small and medium heat shock protein family and autophagy-related genes in hippocampus were detected by real-time PCR, and the expression of HSPB8, BAG3, LC3 and P62 was detected by Western blot. Results 1) The body weight and fasting blood sugar of db/db mice were significantly higher than those of db/m mice(P＜0.01). 2)Small heat shock protein family B [small] member 1-10 (HSPB1-10) gene was expressed in the hippocampus of mice. The expression of HSPB1, HSPB3, HSPB5, HSPB6 and HSPB8 in db/db group was significantly lower than that in db/m group(P＜0.05); the expression of HSPB2, HSPB9 and HSPB10 in db/db group was higher than that in db/m group(P＜0.05); the expression of LC3 in db/db mice was higher than that in db/m group(P＜0.01), whereas the expression of P62 was lower than that of db/m(P＜0.01). 3)The expression of HSPB8, BAG3 and LC3-Ⅱ increased in db/db group(P＜0.01), but the expression of P62 decreased(P＜0.05). Conclusions All sHSPs are expressed in the hippocampus of mice, but the expression profile is different. The expression of HSPB8 protein is increased and autophagy is activated in the hippocampus of 8-week-old diabetic mice.

Key words: diabetes mellitus, small heat shock protein, autophagy, db/db mice

中图分类号:

R587.2

李金凤, 李小承, 殷小红, 马卫国, 李亚. 小热休克蛋白家族和自噬相关蛋白在自发性2型糖尿病db/db小鼠海马组织中的表达[J]. 基础医学与临床, 2020, 40(8): 1059-1063.

LI Jin-feng, LI Xiao-cheng, YIN Xiao-hong, MA Wei-guo, LI Ya. Expression of small heat shock protein family and autophagy related proteins in the hippocampus of db/db mice with spontaneous type 2 diabetes[J]. Basic & Clinical Medicine, 2020, 40(8): 1059-1063.

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