高脂饮食诱导小鼠血糖增高损伤睾丸微血管功能

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (2): 213-217.

高脂饮食诱导小鼠血糖增高损伤睾丸微血管功能

张晓艳¹,李炳蔚²,刘明明²,尚飞¹,刘淑英¹,盛有明²,李宏伟²,修瑞娟²

1. 中国医学科学院北京协和医学院微循环研究所
2. 中国医学科学院微循环研究所

收稿日期:2017-09-28 修回日期:2017-12-15 出版日期:2018-02-05 发布日期:2018-01-24
通讯作者: 修瑞娟 E-mail:xiurj@imc.pumc.edu.cn
基金资助:
1. 2015年中国医学科学院公益性科研院所基本科研业务专项基金。2. 2016年中国医学科学院医学与健康科技创新工程经费资助

High fat diet-induced elevation of blood glucose impairs the microvascular function of testis in mice

Received:2017-09-28 Revised:2017-12-15 Online:2018-02-05 Published:2018-01-24

摘要/Abstract

摘要： 目的观察高脂饮食诱导C57BL/6雄性小鼠血糖升高对睾丸微血管功能及雄性生殖的影响。方法将小鼠随机分为对照组（control）和高脂组（HFD），各20只。HFD组高脂饮食20周,每周测血糖及体质量；腹腔注射伊文思蓝观察血睾屏障通透性；激光多普勒检测睾丸微循环血流量及微血管自律运动频率和振幅；HE染色观察睾丸组织形态；免疫组化检测睾丸微血管血小板内皮细胞黏附分子-1（CD31）及生精细胞增殖细胞核抗原PCNA表达；TUNEL染色法观察生精细胞凋亡。结果 HFD组小鼠体质量及血糖均高于对照组（P＜0.01）；血睾屏障完整性破坏，生精小管通透性增高；睾丸平均血流灌注水平及微血管自律运动频率和振幅均低于对照组（P＜0.01）；生精上皮细胞数量减少、生精上皮变薄；微血管内皮细胞CD31及生精细胞PCNA表达水平均低于对照组（P＜0.01）；生精细胞凋亡水平高于对照组（P＜0.01）。结论高脂饮食可诱导小鼠血糖水平增高致睾丸微血管损伤破坏血睾屏障完整性使小鼠生精上皮结构受损。

关键词: 【关键词】高脂, 高血糖, 睾丸微血管, 血睾屏障, 生精细胞损伤

Abstract: High fat diet-induced elevation of blood glucose impairs the microvascular function of testis in C57BL/6 mice ZHANG Xiao-yan, LI Bing-wei, SHANG Fei, LIU Shu-ying, SHENG You-ming, LI Hong-wei, XIU Rui-juan1* （Institute of Microcirculation, Peking Union Medical College, Chinese Academy of Medical Sciences , Key Laboratory of Microcirculation, Ministry of Health, CAMS & PUMC, Beijing, 100005, China） Abstract：Objective To observe the effects of high fat diet-induced elevation of blood glucose on the microvascular function of testis and male reproduction in C57BL/6 mice. Methods A total of 40 male C57BL/6 mice were randomly divided into control group and high fat group(High Fat Diet，HFD)，n = 20. The mice in control group were fed with normal diet, and the mice in HFD group were fed with high fat diet for 20 weeks. Blood glucose and body weight were measured weekly. Testicular blood testosterone barrier permeability was evaluated by intraperitoneal injection of Evans blue. The blood flow of testicular microcirculation and the testicular microvascular vasomotion were detected by laser Doppler blood flow imaging system. The expression of CD31 on testicular microvascular endothelial cells was detected by HE staining and immunohistochemistry. The proliferation and apoptosis of spermatogenic cells were observed by immunohistochemistry and TUNEL (TdT-mediated dUTP Nick-End Labeling) staining. Results The body weight and blood glucose of HFD group were significantly higher than those in control group (P <0.01). Evans blue staining showed that the integrity of blood testis barrier of HFD group was damaged, and red fluorescent substance was observed in seminiferous tubules. In HFD group, the mean blood flow of testis and the frequency and amplitude of microvascular vasomotion were significantly lower than those in control group(P<0.01). HE staining showed that the number of spermatogenic epithelial cells and the thickness of seminiferous epithelium were decreased than those in control group. The expression of CD31 in microvascular endothelial cells was significantly lower in HFD group than that in control group (P<0.01). The expression of PCNA expression in spermatogenic cells was lower than that in the control group (P <0.05), and the apoptosis level evaluated by TUNEL staining was higher than that in the control group (P <0.05). Conclusion Increased blood glucose level induced by high fat diet in mice can impair the testicular microvasculature and damage the integrity of blood testosterone barrier and injure the structure of seminiferous epithelium in mice.

Key words: Key words: High fat diet, Hyperglycemia, Testicular microvascular, Blood testicular barrier, Spermatogenic cells injury

中图分类号:

R392.12

张晓艳李炳蔚刘明明尚飞刘淑英盛有明李宏伟修瑞娟. 高脂饮食诱导小鼠血糖增高损伤睾丸微血管功能[J]. 基础医学与临床, 2018, 38(2): 213-217.