关键词: {关键词}：大肠癌, TGF-β1, EMT

Abstract: Objective To explore the relationship between the EMT cells and non-EMT cells in colorectal cancer metastasis. Methods TGF-β1 has stimulated colon cancer cells HT29 for a long time.The expressions of Ecadherin and Vimentin mRNA of cells were detected by fluorescence quantitative PCR. The morphology and Cytoskeleton of HT29 and HT29/ TGF-β1 cells were observed by HE and Coomassie blue staining respectively, the green and red fluorescent protein plasmid were transfected into EMT and non-EMT cells respectively, then mixed culturing two kind cells, doing scratch test, observing the movement of two kinds of cells. Results After continuous stimulated by TGF-β1 for 10d, the expression of Ecadherin mRNA decreased with significant statistical difference, the expression of Vimentin mRNA increased with significant statistical difference（P<0.05）. After continuous stimulated by TGF-β1 for 40d, HT29 cells change spindle, decline cell-cell adhesion. HT29 cells had more surface projections, and less cytoskeletal structures and spot-like actin bodies than HT29/ TGF-β1 cells. Scratch test shows that the, migration rate of the HT29 cells after stimulation is the fastest, HT29 cells is the slowest.Co-culturing two kinds of red and green fluorescent plasmid DNA transfected cells, scratches, we can see the red and green cells evenly distributed. Conclusion TGF-β1 can induce colorectal cancer cells EMT；In colorectal cancer, the EMT cells can enhance invasion and metastasis of the non-EMT cells.

Key words: Key words:colorectal cancer, EMT, TGF-β1