GLP-1受体激动剂Exenatide改善糖尿病大鼠心血管功能

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (9): 1171-1175.

GLP-1受体激动剂Exenatide改善糖尿病大鼠心血管功能

洪霓¹,赵倩²,刘腾³,张辉³,刘琦⁴,王云⁴,王雯⁵

1. 中国医学科学院基础医学研究所
2. 北京大学首钢医院
3. 首都医科大学基础医学院
4. 首都医科大学附属北京同仁医院干部医疗科
5. 首都医科大学基础医学院病理生理教研室

收稿日期:2013-05-23 修回日期:2013-06-24 出版日期:2013-09-05 发布日期:2013-08-28
通讯作者: 王雯 E-mail:wangwen@ccmu.edu.cn
基金资助:
首都医科大学基础-临床科研合作基金

Exenatide improves cardiovascular function in diabetic rats

Received:2013-05-23 Revised:2013-06-24 Online:2013-09-05 Published:2013-08-28

摘要/Abstract

摘要： 目的探讨胰高血糖素样肽1(GLP－1) 受体激动剂Exenatide（Ex）治疗对糖尿病大鼠心血管功能的影响，为其临床应用提供实验依据。方法用单因素四水平不等重复的完全随机实验进行研究，34只Wistar 大鼠随机分成4 组:对照组（C, n=7）, 糖尿病组( DM, n=9)，小剂量Ex治疗组(Emin， n=9)，大剂量Ex治疗组(Emax，n=9)。检测大鼠空腹血糖水平；使用小动物超声仪观察大鼠左心室射血分数（EF）、左心室短轴缩短率（FS）；观察腹主动脉血流速(AF)，胸主动脉内膜扫描电镜观察血管内膜损伤。结果 DM组空腹血糖明显升高（P<0.01），Exenatide治疗组空腹血糖较DM组显著降低（P<0.05）。DM组EF、FS较对照明显降低（P<0.01），Exenatide治疗组EF、FS明显升高（P<0.05）。DM组腹主动脉血流速明显降低（P<0.01），血管内膜出现明显损伤； Exenatide治疗组腹主动脉血流速较DM组明显升高(分别P<0.05, P<0.01)，血管内膜损伤减轻。结论 Exenatide能够降低糖尿病大鼠血糖水平, 并改善糖尿病心脏和血管功能。

关键词: 关键词： Exenatide, 糖尿病, 大鼠, 心血管功能

Abstract: Objective To investigate the effect of Exenatide (Ex), a glucagon-like peptide-1 (GLP-1) receptor, on cardiovascular function in diabetic rats, and to provide the theoretical foundation for the clinical therapy. Methods A completely randomized design with four treatments was employed in this study. Thirty four Wistar rats were randomly divided into four groups, including control (C, n=7); diabetic model (DM, n=9,); low dose of Ex intake (Emin, n=9);. high dose of Ex intake (Emax, n=9). The level of fasting plasma glucose was determined. Ejection fraction (EF) and the fractional shortening (FS) of the left ventriculus of the rats were determined by using ultrasound for small animals. The blood velocity of abdominal aortic flow (AF) was measured. The injuries in the endangium of thoracic aorta were inspected by using the thoracic scanning electron microscope. Results The level of fasting plasma glucose was significantly higher (P<0.01) in rats from DM group than from control group. The rats in exenatide treatment group had a lower level of fasting plasma glucose than those in DM group (P <0.05). The rats of DM group had a more striking dysfunction either in ejection fraction or fractional shortening compared with control group (P <0.01), and attenuated levels than exenatide treatment group (P <0.05). In addition, the blood velocity of abdominal aortic flow in rats from DM group was obviously slower in control group (P <0.01), and vascular intimals of rats from former group were tremendously damaged. The blood velocity of abdominal aortic flow in rats from exenatide treatment group was much faster than DM group, the level of vascular intimal injury was alleviated. Conclusion In addition to reducing the level of glucose, exenatide can also ameliorate cardiovascular dysfunction in diabetic rats.

Key words: Key Words: Exenatide, diabetes mellitus, rats, cardiovascular function

中图分类号:

R363.1

洪霓赵倩刘腾张辉刘琦王云王雯. GLP-1受体激动剂Exenatide改善糖尿病大鼠心血管功能[J]. 基础医学与临床, 2013, 33(9): 1171-1175.

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