泽泻提取物改善妊娠期糖尿病模型大鼠血糖、脂质及氧化应激水平

doi:10.16352/j.issn.1001-6325.2025.11.1463

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (11): 1463-1472.doi: 10.16352/j.issn.1001-6325.2025.11.1463

泽泻提取物改善妊娠期糖尿病模型大鼠血糖、脂质及氧化应激水平

宋春蕊¹, 李敬^2*

1.北京市大兴区人民医院妇产科,北京 102600;
2.中国中医科学院广安门医院南区急诊科,北京 102600

收稿日期:2024-05-13 修回日期:2024-11-04 出版日期:2025-11-05 发布日期:2025-10-24
通讯作者: ^*heartgarden83@sina.com

Effects of Alisma orientalis extract on blood glucose, lipid and oxidative stress of pregnant rat models with diabetes

SONG Chunrui¹, LI Jing^2*

1. Department of Obstetrics and Gynaecology, Beijing Daxing District People's Hospital,Beijing 102600;
2. Department of Emergency, Guang'anmen Hospital South Campus, China Academy of Chinese Medical Sciences,Beijing 102600,China

Received:2024-05-13 Revised:2024-11-04 Online:2025-11-05 Published:2025-10-24
Contact: ^*heartgarden83@sina.com

摘要/Abstract

摘要： 目的探讨泽泻提取物(zexie)通过调控miR-4417/TGF-β通路对妊娠期糖尿病大鼠的治疗效果及作用机制。方法将妊娠期糖尿病(GDM)模型大鼠随机分为糖尿病组、泽泻低、中、高剂量组和胰岛素组、miR-NC组、miR-4417 mimic组、miR-4417 inhibitor组、泽泻+miR-4417 mimic+LY364947组,每组12只,另选12只正常妊娠大鼠为正常组。HE染色检测大鼠胰岛组织病理损伤。RT-qPCR 和Western blot检测组织中各因子mRNA和蛋白质水平。结果与糖尿病组相比,泽泻低、中、高和胰岛素组的血糖、血脂、氧化应激指标及胰岛组织病理损伤显著改善(P＜0.05),miR-4417和Tgf-β mRNA显著降低(P＜0.05),其中泽泻高剂量组变化最为显著(P＜0.05)。与miR-NC组相比,miR-4417 mimic组中TGF-β蛋白明显升高(P＜0.05),miR-4417 inhibitor 组明显降低(P＜0.05)。与糖尿病组相比,泽泻组、泽泻+miR-4417 mimic组、泽泻+miR-4417 mimic+ LY364947组的氧化应激和ROS 累积显著降低(P＜0.05)。与miR-4417 mimic组比较,泽泻+miR-4417 mimic组氧化应激和活性氧(ROS)累积显著降低(P＜0.05)。泽泻组与泽泻+miR-4417 mimic+ LY364947组比较差异无统计学意义。结论泽泻能够改善大鼠的血糖和脂质水平,降低胰岛素抵抗,控制氧化应激及氧化损伤的能力,其机制可能与miR-4417/TGF-β通路的调节有关。

关键词: 泽泻, 妊娠期糖尿病, TGF-β通路, miR-4417

Abstract: Objective To investigate the therapeutic effects and mechanisms of Alisma orientale extract(zexie) on gestational diabetes mellitus (GDM) rats by regulating the miR-4417/TGF-β pathway. Methods GDM model rats were randomly divided into a diabetic group, low,medium,high-dose zexie group, insulin group, miR-NC group, miR-4417 mimic group, miR-4417 inhibitor group, and zexie+miR-4417 mimic+LY364947 group, with 12 rats in each group. Additionally, 12 normal pregnant rats served as the control group. Hematoxylin and eosin(HE) staining was used to evaluate pancreatic islet pathology. RT-qPCR and Western blot were employed to measure the mRNA and protein levels of various factors. Results Compared with the diabetic group, the glucose, lipid, oxidative stress indicators, and pancreatic islet pathological damage were significantly improved(P＜0.05) in the low, medium and high-dose zexie groups and the insulin group. The expression levels of miR-4417 and TGF-β mRNA were notably reduced(P＜0.05), with the most significant changes observed in the high-dose zexie group(P＜0.05). Compared with the miR-NC group, the TGF-β protein level was significantly increased(P＜0.05) in the miR-4417 mimic group and decreased(P＜0.05) in the miR-4417 inhibitor group. Compared with the diabetic group, the oxidative stress and reactive oxygen species (ROS) accumulation were significantly lower(P＜0.05) in the zexie, zexie+miR-4417 mimic, and zexie+miR-4417 mimic+LY364947 groups. Compared with the miR-4417 mimic group, the oxidative stress and ROS accumulation were significantly reduced(P＜0.05) in the zexie+miR-4417 mimic group. No significant differences were found between the zexie group and the zexie+miR-4417 mimic + LY364947 group. Conclusions Zexie can improve glucose and lipid levels, reduce insulin resistance, and control oxidative stress and damage in GDM rats, possibly through the regulation of the miR-4417/TGF-β pathway.

Key words: Alisma orientalis, gestational diabetes, TGF-β pathway, miR-4417

中图分类号:

R714.25

宋春蕊, 李敬. 泽泻提取物改善妊娠期糖尿病模型大鼠血糖、脂质及氧化应激水平[J]. 基础医学与临床, 2025, 45(11): 1463-1472.

SONG Chunrui, LI Jing. Effects of Alisma orientalis extract on blood glucose, lipid and oxidative stress of pregnant rat models with diabetes[J]. Basic & Clinical Medicine, 2025, 45(11): 1463-1472.

参考文献

[1] Ugwudike B, Kwok M. Update on gestational diabetes and adverse pregnancy outcomes[J]. Curr Opin Obstet Gynecol, 2023, 35:453-459.
[2] 58th EASD Annual Meeting of the European Association for the Study of Diabetes: Stockholm, Sweden, 19 - 23 September 2022[J]. Diabetologia, 2022, 65:1-469.
[3] Zhao LL, Zhao WQ, Zhao ZY, et al. Rapid discrimina-tion of Alismatis Rhizoma and quantitative analysis of triterpenoids based on near-infrared spectroscopy[J]. Spectrochim Acta A Mol Biomol Spectrosc, 2024,321:124618. doi: 10.1016/j.saa.2024.124618.
[4] Zhang Y, Jiang K, Liu P, et al. Mechanism of triterpenoids from Alismatis Rhizoma against liver fibrosis based on an integrated approach using network pharmacology, molecular docking, and luciferase assay[J].Nat Prod Res, 2023,37:3826-3831.
[5] Venz S, Junker H, Ultsch E, et al. Identification of the regulatory targets of miR-3687 and miR-4417 in prostate cancer cells using a proteomics approach[J]. Int J Mol Sci, 2022, 23:10565. doi: 10.3390/ijms231810565.
[6] Wen B, Liao H, Lin W, et al.The role of TGF-β during pregnancy and pregnancy complications[J]. Int J Mol Sci, 2023, 24:16882.doi: 10.3390/ijms242316882.P
[7] Wu CH,Hsiao YM,Yeh KT,et al.Upregulation of microRNA-4417 and its target genes contribute to nickel chloride-promoted lung epithelial cell fibrogenesis and tumori-genesis[J].Sci Rep, 2017, 7:15320. doi: 10.1038/s41598-017-14610-7.
[8] 张艳红,王柯静,李丽娟,等.葫芦巴碱对妊娠期糖尿病大鼠血脂代谢及胰岛素抵抗的影响[J].中国比较医学杂志, 2023, 33:68-74.
[9] Hagar HH, Alhazmi SM, Arafah M, et al. Inhibition of sepsis-induced pancreatic injury by leukotriene receptor antagonism via modulation of oxidative injury, and downregulation of inflammatory markers in experimental rats[J]. Naunyn Schmiedebergs Arch Pharmacol, 2024, 397:3425-3435.
[10] Zhou X, Luo J, Lin S, et al. Efficacy of Poria cocos and Alismatis rhizoma against diet-induced hyperlipidemia in rats based on transcriptome sequencing analysis[J]. Sci Rep, 2023, 13:17493. doi: 10.1038/s41598-023-43954-6.
[11] Song L, Zhang W, Chang Z,et al. miR-4417 targets tripartite motif-containing 35 (TRIM35) and regulates pyruvate kinase muscle 2(PKM2) phosphorylation to promote proliferation and suppress apoptosis in hepatocellular carcinoma cells[J]. Med Sci Monit,2017,23:1741-1750. doi: 10.12659/msm.900296.
[12] Tang XW, Qin QX. miR-335-5p induces insulin resis-tance and pancreatic islet β-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-β signaling pathway[J]. J Cell Physiol, 2019, 234:6654-6666.

泽泻提取物改善妊娠期糖尿病模型大鼠血糖、脂质及氧化应激水平

Effects of Alisma orientalis extract on blood glucose, lipid and oxidative stress of pregnant rat models with diabetes

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