pSilencer-VEGF-siRNA诱导骨肉瘤细胞系MG63凋亡并上调caspase-3表达

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (4): 352-355.

pSilencer-VEGF-siRNA诱导骨肉瘤细胞系MG63凋亡并上调caspase-3表达

高悠水张立智钱光童天朗胡牧梅炯蔡宣松

同济大学附属同济医院同济大学附属同济医院同济大学附属同济医院

收稿日期:2008-05-14 修回日期:2008-08-07 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 梅炯

Upregulation of caspase-3 expression and apoptosis of cell line MG63 induced by pSilencer-VEGF-siRNA transfection

You-shui GAO, Li-zhi ZHANG, Guang QIAN, Tian-lang TONG, Mu HU, Jiong MEI, Xuan-song CAI

Tongji Hospital, Tongji University Tongji Hospital, Tongji University Tongji Hospital, Tongji University

Received:2008-05-14 Revised:2008-08-07 Online:2009-04-25 Published:2009-04-25
Contact: Jiong MEI,

摘要/Abstract

摘要： 目的研究pSilencer-VEGF-siRNA转染骨肉瘤细胞系MG63后诱导其凋亡以及对capase-3表达能力的影响。方法体外常规培养骨肉瘤细胞系MG63并构建人类特异性的pSilencer-VEGF-siRNA；分为pSilencer-VEGF-siRNA转染组和空载体组。转染后48~72 h，流式细胞仪Annexin V-FITC/PI 染色检测细胞凋亡、分析细胞周期；Western blotting法测定caspase-3表达。结果 MG63转染pSilencer-VEGF-siRNA表达质粒后，早期出现细胞凋亡及明显的G1期阻滞以及G1期细胞数增加；pSilencer-VEGF转染组caspase-3的表达较空载体组和对照组明显上调。结论 pSilencer-VEGF-siRNA可诱导MG63细胞早期凋亡；并可上调caspase-3的表达。

关键词: 血管内皮生长因子, 基因沉默, 骨肉瘤, 凋亡, MG-63, Caspase-3

Abstract: Objective To research effects on caspase-3 expression of osteosarcoma cell line MG63 induced by pSilencer-VEGF-siRNA transfection. Methods The cell line MG63 was cultured and human-specific pSilencer-VEGF -siRNA was constructed. The experiment was divided into pSilencer-VEGF-siRNA transfection and vector group. Apoptosis and cell cycle of MG63 was detected and analysesed with conjugated annexin-V (Annexin-V-FITC) antibody and propidium iodide (PI) 48~72 hours after transfection with flow cytometry, and the expression of caspase-3 was detected through Western blotting. Results The early apoptosis of MG63 could be achieved after transfection and with a blockage of G1 phase and cell accumulation occurring. Caspase-3 expression was up-regulated apparently in the transfection group compared with vector group. Conclusion Early apoptosis of MG63 could be induced by pSilencer-VEGF-siRNA, and up-regulation of caspase-3 expression could be achieved.

Key words: Vascular endothelial growth factor (VEGF), Gene silencing, Osteosarcoma, Apoptosis, MG-63, Caspase-3

高悠水张立智钱光童天朗胡牧梅炯蔡宣松. pSilencer-VEGF-siRNA诱导骨肉瘤细胞系MG63凋亡并上调caspase-3表达[J]. 基础医学与临床, 2009, 29(4): 352-355.

You-shui GAO; Li-zhi ZHANG; Guang QIAN; Tian-lang TONG; Mu HU; Jiong MEI; Xuan-song CAI. Upregulation of caspase-3 expression and apoptosis of cell line MG63 induced by pSilencer-VEGF-siRNA transfection[J]. Basic & Clinical Medicine, 2009, 29(4): 352-355.

[1]	刘雯霞刘彩红郭蕊孟芝君高佳王亚静. 尼古丁促进高糖/高脂培养的大鼠心肌细胞系H9C2凋亡[J]. , 2020, 40(1): 24-29.
[2]	蒋庆锋于永魁程金华申思宁邢文群. lncRNA HEIH通过miR-98-5p调节肺癌细胞增殖和凋亡[J]. , 2020, 40(1): 30-36.
[3]	苗玉娟王淑坤孙丹丁康刘美逸张妍周风华. miR-181 通过 Prox1 促进人乳腺癌细胞系MCF-7的增殖并抑制凋亡[J]. 基础医学与临床, 2019, 39(9): 1265-1269.
[4]	童静凯江霞. 托伐普坦对小鼠内髓集合管细胞系水通道蛋白及肾小管功能的影响[J]. 基础医学与临床, 2019, 39(8): 1163-1167.
[5]	晋瑞金迎迎李嫚雷喆. 过表达脾酪氨酸激酶降低氧化应激诱导的小鼠成骨细胞凋亡[J]. 基础医学与临床, 2019, 39(7): 1013-1018.
[6]	任玮余宏川王萍刘英茹. miR-17-5p诱导小鼠肾足细胞系凋亡[J]. 基础医学与临床, 2019, 39(7): 973-977.
[7]	雷晓燕孙永红陈星星崔维静刘璟. 乙肝病毒X蛋白抑制小鼠足细胞系MPC5增殖并促进其凋亡[J]. 基础医学与临床, 2019, 39(5): 617-622.
[8]	马从乾王雅王娜杨柯陈德才. miR-181a过表达加重H2O2诱导的HUVECs损伤[J]. 基础医学与临床, 2019, 39(5): 690-695.
[9]	王圣凯胡苏琴吴玲吴杭菲郑靖宇蒋自培李剑敏. miR-138在乙醇诱导的大鼠心肌细胞系H9C2氧化应激中表达增加[J]. 基础医学与临床, 2019, 39(5): 677-681.
[10]	战仕胜李军杨婷婷王雅蓉王秀青. 抗菌肽merecidin诱导人肺腺癌细胞系A549凋亡[J]. 基础医学与临床, 2019, 39(4): 473-477.
[11]	刘莹张艳丽王秀芳. 沉默FOXO1抑制H2O2诱导的大鼠肺泡2型上皮细胞凋亡[J]. 基础医学与临床, 2019, 39(4): 519-523.
[12]	张娟冯燕和水祥. 过表达miR-5195-3p对结直肠癌细胞凋亡的影响[J]. 基础医学与临床, 2019, 39(3): 385-391.
[13]	于婧王凤琴姚文娟吴冬梅刘大鹏. 甲状腺乳头状癌BRAFV600E基因突变对VEGF和MMP-9表达的影响?[J]. , 2019, 39(11): 1578-1582.
[14]	张许梅王瑾霍海燕岳继萍张文婷焦向英. 血管紧张素II 1型受体自身抗体可上调TXNIP导致大鼠胰岛β细胞系INS-1凋亡[J]. , 2019, 39(11): 1548-1555.
[15]	宁娜侯晓鸿李杨何金玲燕子王晓晖王丽. 自噬降低激活β1-AA诱导的心肌细胞内质网应激相关凋亡途径[J]. 基础医学与临床, 2019, 39(1): 1-6.