视神经切断联合高眼压增高大鼠视网膜内p75NTR和Sortilin的蛋白表达水平

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (4): 347-351.

视神经切断联合高眼压增高大鼠视网膜内p75NTR和Sortilin的蛋白表达水平

鹿宁宁王宁利魏勇丁静文韩松李俊发

首都医科大学附属北京同仁医院首都医科大学附属北京同仁医院首都医科大学附属北京同仁医院首都医科大学神经生物系首都医科大学神经生物系

收稿日期:2008-12-26 修回日期:2009-01-16 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 李俊发

Optic nerve transaction combined with high intraocular pressure increased the protein expression levels of p75NTR and sortilin in retina of rats

Beijing Tongren Hospital,Capital Medical University Beijing Tongren Hospital,Capital Medical University Beijing Tongren Hospital,Capital Medical University Department of Neurobiology and Beijing Institute for Neuroscience, Capital Medical University Department of Neurobiology and Beijing Institute for Neuroscience, Capital Medical University

Received:2008-12-26 Revised:2009-01-16 Online:2009-04-25 Published:2009-04-25

摘要/Abstract

摘要： 目的观察视神经切断联合高眼压状态下，视网膜内p75NTR和Sortilin蛋白表达水平的变化。方法：60只健康雄性Wistar大鼠,随机分为对照(control，n=6)、假手术(sham，n=6)、单纯视神经切断(NT，n=24)和视神经切断联合高眼压(NP，n=24)4组。NT和NP模型组，进一步分为术后第1、3、7和14天组(均n=6)。用Western blot法，检测各组大鼠视网膜内p75NTR和Sortilin蛋白表达。结果 NP组大鼠视网膜内，p75NTR及Sortilin蛋白表达量在术后第3、7和14天明显高于对照组、假手术组及单纯视神经切断组(p<0.05)。结论高眼压可使大鼠视网膜内RGC本身合成的Sortilin和p75NTR蛋白量增高， 60/50ku的p75NTR，以及90ku成熟Sortilin蛋白可能参与了高眼压所致大鼠视网膜神经节细胞的损伤。

关键词: 视神经切断, 高眼压, 青光眼, p75NTR, Sortilin

Abstract: Objective To observe the changes in sortilin and p75NTR protein expressions in retina of rats following optic nerve transaction combined with high intraocular pressure. Methods Sixty healthy male Wistar rats, were randomly divided into 4 groups: the control (control, n=6), sham (n=6), optic nerve transaction (NT, n=24), and NT combined with high intraocular pressure (NP, n=24) groups. In addition, the NT and NP groups were subdivided into 1, 3, 7 and 14 days' group (n=6 for each group). The p75NTR and sortilin protein expression levels were determined by the quantitative analysis of Western blot. Results The protein expressions of p75NTR, and sortilin increased significantly (p<0.05) in retina of rats from NP group at day 3, 7 and 14 when compared with control, sham and NT groups. Conclusion The increased p75NTR and sortilin protein expressions in retina of rats following high intraocular pressure came from retinal RGC themselves.These results also suggested that the enhanced sortilin (90ku) and p75NTR (60/50ku) protein expressions might be involved in the high ocular pressure-induced retinal RGC injuries of rats.

Key words: Optic nerve transaction, High intraocular pressure, Glaucoma, p75NTR, Sortilin

中图分类号:

R339.5

鹿宁宁王宁利魏勇丁静文韩松李俊发. 视神经切断联合高眼压增高大鼠视网膜内p75NTR和Sortilin的蛋白表达水平[J]. 基础医学与临床, 2009, 29(4): 347-351.

. Optic nerve transaction combined with high intraocular pressure increased the protein expression levels of p75NTR and sortilin in retina of rats[J]. Basic & Clinical Medicine, 2009, 29(4): 347-351.