2型糖尿病患者单核巨噬细胞胆固醇外流功能的降低与ABCG1表达下调相关

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (12): 1314-1319.

2型糖尿病患者单核巨噬细胞胆固醇外流功能的降低与ABCG1表达下调相关

王会娟陈连凤方全严晓伟

中国医学科学院北京协和医学院北京协和医院心内科中国医学科学院北京协和医学院北京协和医院心内科中国医学科学院北京协和医学院北京协和医院心内科中国医学科学院北京协和医学院北京协和医院心内科

收稿日期:2009-08-31 修回日期:2009-09-21 出版日期:2009-12-20 发布日期:2009-12-20
通讯作者: 严晓伟

Impaired cholesterol efflux in macrophages of patients with type 2 diabetes mellitus is associated with the decreased expression of ABCG1

Hui-juan WANG, Lian-feng CHEN, Quan FANG, Xiao-wei YAN

Dept. of Cardiology, PUMC Hospital, CAMS & PUMC Dept. of Cardiology, PUMC Hospital, CAMS&PUMC Dept. of Cardiology, PUMC Hospital, CAMS&PUMC

Received:2009-08-31 Revised:2009-09-21 Online:2009-12-20 Published:2009-12-20
Contact: Xiao-wei YAN

摘要/Abstract

摘要： 目的研究2型糖尿病患者巨噬细胞内胆固醇外流的变化及ABCA1、ABCG1和SR-B1的表达情况。方法选取2型糖尿病患者30例（包括合并冠心病组与单纯糖尿病组各15例）和正常对照组15例。分离外周血单核细胞进行巨噬化培养，应用Real-time PCR与Western blot方法测定细胞ATP结合盒受体A1（ABCA1）、ATP结合盒受体G1（ABCG1）和清道夫受体B1（SR-B1）的mRNA及蛋白表达。以5%自身血清及标准血清为接受体测定巨噬细胞胆固醇流出率。结果ABCG1在2型糖尿病患者巨噬细胞的mRNA与蛋白表达显著低于对照组，而ABCA1与SR-B1的表达在各组间无差异。2型糖尿病患者巨噬细胞的胆固醇流出率也显著低于对照组（P＜0.01）；ABCG1的mRNA表达与巨噬细胞胆固醇外流率呈显著正相关（r=0.552，r=0.630,P＜0.01）。结论 2型糖尿病患者巨噬细胞的胆固醇外流功能明显降低，与ABCG1表达下调相关。

关键词: 胆固醇外流, ABCG1, ABCA1, SR-B1, 糖尿病

Abstract: Objective In the present study, we examined the cholesterol efflux and the expressions of ATP-binding cassette receptor A1 (ABCA1), ABCG1 and scavenger receptor B1 (SR-B1) in monocytes derived macrophages of patients with type 2 diabetes mellitus. Methods and Results Blood was collected from subjects with and without Type 2 diabetes mellitus. Peripheral blood monocytes were differentiated for 72 hours into macrophages, and cholesterol efflux assays, Real-time quantitative PCR and western blot were performed. Macrophages from patients with type 2 diabetes mellitus had a reduction in cholesterol efflux relative to healthy controls. The mRNA and protein expressions of ABCG1 in macrophages from patients with type 2 diabetes mellitus were significantly reduced compared with control subjects. In contrast, the expression of ABCA1 and SR-B1 were similar in both control subjects and diabetic patients. In addition, cellular cholesterol efflux from macrophages to autologous serum and pool serum was significantly correlated with the expression of ABCG1. Conclusion ABCG1 expression and cholesterol efflux are reduced in patients with type 2 diabetes mellitus. This impaired cholesterol efflux significantly correlates with decreased expression of ABCG1.

Key words: cholesterol efflux, ABCA1, ABCG1, SR-B1, diabetes mellitus

王会娟陈连凤方全严晓伟. 2型糖尿病患者单核巨噬细胞胆固醇外流功能的降低与ABCG1表达下调相关[J]. 基础医学与临床, 2009, 29(12): 1314-1319.

Hui-juan WANG; Lian-feng CHEN; Quan FANG; Xiao-wei YAN. Impaired cholesterol efflux in macrophages of patients with type 2 diabetes mellitus is associated with the decreased expression of ABCG1[J]. Basic & Clinical Medicine, 2009, 29(12): 1314-1319.

[1]	黄红莉梁淑贞林茂陈继冰牛立志. 糖尿病患者血液细胞来源多能干细胞诱导分化为胰岛素分泌细胞[J]. 基础医学与临床, 2019, 39(4): 529-535.
[2]	蔡小玲巩慧玲杨波徐倩哈小琴. 糖尿病前期新的标志物及预防[J]. 基础医学与临床, 2019, 39(2): 268-271.
[3]	邱维张薇陶建瓴王海云郑法雷李雪梅. 倍半萜内酯减轻糖尿病小鼠胰岛素抵抗及早期肾损伤[J]. 基础医学与临床, 2018, 38(9): 1292-1297.
[4]	何秀娟林燕李萍. 中性粒细胞胞外诱捕网与糖尿病足溃疡的研究进展[J]. 基础医学与临床, 2018, 38(3): 409-412.
[5]	周路平陈娇徐勇黄炜龙洋丁静雅秦露丹. RIPK2介导自噬对高糖诱导的小鼠肾系膜细胞ROS、caspase-1及IL-1β表达的激活作用[J]. 基础医学与临床, 2018, 38(10): 1389-1396.
[6]	王丽萍宋斌戴燕陈晖张真稳闫彩凤潘云龙. 多种脂源性细胞因子与2型糖尿病患者胰岛素抵抗相关[J]. 基础医学与临床, 2017, 37(9): 1247-1250.
[7]	李爱玲彭睿孙艳彭惠民易红张政. 长链非编码RNA-1700020I14Rik对高糖培养下肾系膜细胞纤维化的影响[J]. 基础医学与临床, 2017, 37(6): 781-785.
[8]	刘滢贺桂琼覃万翔罗敏汪克建. β淀粉样蛋白及代谢酶类在阿茨海默病和糖尿病模型小鼠脑内的表达[J]. 基础医学与临床, 2017, 37(5): 653-657.
[9]	孙莹梁晓春刘伟刘頔. 筋脉通含药血清对高糖培养大鼠雪旺细胞β-catenin、GSK-3β及髓鞘零蛋白表达的影响[J]. 基础医学与临床, 2017, 37(4): 484-487.
[10]	宋喜发陆琴吴少泽张素勤. 低氧诱导因子减轻高糖条件下苯肾上腺素诱导大鼠H9C2心肌细胞肥大[J]. 基础医学与临床, 2017, 37(11): 1622-1624.
[11]	金成吉唐田于晓静曲建梅段薇张弢. 高糖及高胰岛素对人血管平滑肌细胞增殖、迁移及miRNA-145水平的影响[J]. 基础医学与临床, 2017, 37(1): 94-98.
[12]	文利陈蕾孙一瑄段青蕊魏礼江周琴彭惠民张政. 柚皮素通过抑制TGF-β1/smad信号传导通路缓解糖尿病肾病大鼠肾损伤[J]. 基础医学与临床, 2016, 36(7): 896-901.
[13]	庞云程勇伍鑫唐康. 结直肠癌合并糖尿病患者组织中EMT相关蛋白的表达[J]. 基础医学与临床, 2016, 36(6): 799-804.
[14]	罗春步世忠王福艳. 葛根素治疗2型糖尿病的药理机制和临床进展[J]. 基础医学与临床, 2016, 36(11): 1582-1585.
[15]	杨久琳刁腾月张岩. db/db小鼠心、肾RAS组分的分子表达[J]. 基础医学与临床, 2015, 35(3): 341-344.