基础医学与临床 ›› 2008, Vol. 28 ›› Issue (8): 846-849.

• 研究论文 • 上一篇    下一篇

高压氧降低缺氧缺血性脑损伤大鼠脑组织内caspase-3表达

檀国军 牛彦平 王玲   

  1. 河北医科大学第二医院 河北医科大学第二医院 哈尔滨医科大学药理学教研室
  • 收稿日期:2007-07-20 修回日期:2007-12-17 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 檀国军

Hyperbaric Oxygen-treatment decreased the expression of Caspase-3 in the brain of Hypoxic-Ischemic injuried rats

Guo-jun TAN, Yan-ping NIU, Ling WAN   

  1. The Second affiliated Hospital,Hebei Medical University
  • Received:2007-07-20 Revised:2007-12-17 Online:2008-08-25 Published:2008-08-25
  • Contact: Guo-jun TAN,

摘要: 目的 探讨高压氧(HBO)对缺氧缺血新生大鼠神经细胞凋亡及Caspase-3表达的影响。方法 建立缺氧缺血性脑损伤(HIBD)大鼠模型,将其分为HIBD组及HBO处理组, 并设立假手术组(n=24)。观察三组大鼠不同时间脑组织病理学改变,同时用免疫组化的方法检测三组大鼠脑组织内Caspase-3蛋白表达的动态变化。结果 HIBD组18、24、48和96h 时间点海马及皮层区Caspase-3蛋白的表达均明显高于假手术组(P<0.05);HBO干预组大鼠Caspase-3表达的升高程度均低于HIBD组(P<0.05)。结论 HBO能降低大鼠海马及皮层区神经细胞Caspase-3蛋白的表达水平。

关键词: Caspase-3, 凋亡, 高压氧, 缺氧缺血性脑损伤

Abstract: Objective To explore the effect of Hyperbaric Oxygen (HBO) on the apoptosis resulted from Hypoxic-Ischemic Brain Damage (HIBD),and to observe the changes in Caspase-3 expression. Methods Rats were divided into HIBD、HBO、sham operation group(n=24).To observe the morphologic change of brain tissues treated by HBO and compared with that of the Hypoxia-ischemia rat model, then check the dynastic change of Caspase-3 expression by immunohistochemistry at different time point. Results The expression of Caspase-3 in the hippocampus and cortex was higher than that of sham group at 18, 24, 48 and 96h time point(P<0.05). The increased Caspase-3 expression decreased significantly in the brain of HIBD rats after HBO treatment (P<0.05). Conclusions HBO decreased the expression of Caspase-3 in hippocampus and cortex of HIBD rats.

Key words: Caspase-3, apoptosis, Hyperbaric Oxygen, Hypoxic-Ischemic Brain Damage