PIAS3敲低对前列腺癌细胞增殖和凋亡的影响

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (5): 469-472.

PIAS3敲低对前列腺癌细胞增殖和凋亡的影响

何旺祝志臻程志强王扬李鸣

北京大学泌尿外科研究所北京大学泌尿外科研究所北京大学临床肿瘤学院北京大学临床肿瘤学院北京大学临床肿瘤学院

收稿日期:2007-09-20 修回日期:2007-10-29 出版日期:2008-05-25 发布日期:2008-05-25
通讯作者: 李鸣

Effects of PIAS3 knocking down on proliferation and apoptosis of prostate carcinoma cell

Institute of Urology, the First Hospital, Peking University School of Oncology,Beijing Cancer Hospital,Peking University

Received:2007-09-20 Revised:2007-10-29 Online:2008-05-25 Published:2008-05-25

摘要/Abstract

摘要： 目的研究PIAS3敲低对人前列腺癌细胞系DU145细胞增殖、细胞周期和凋亡的影响。方法构建PIAS3 shRNA表达质粒pSilencer4.1/PIAS3，转染DU145细胞。MTT法检测细胞增殖，流式细胞仪分析细胞周期和凋亡。结果测序证实PIAS3 RNA表达质粒构建成功。转染后DU145细胞PIAS3蛋白表达明显下调。MTT分析显示PIAS3敲低促进细胞增殖，并存在剂量-效应关系。流式细胞术分析显示：PIAS3敲低后S期细胞比例增加，G0/G1期细胞比例减少，凋亡细胞比例减少。结论 PIAS3敲低促进前列腺癌细胞增殖，抑制其凋亡。

关键词: 前列腺癌, 活化的信号转导子和转录激活子3的蛋白抑制剂, RNA干扰, 凋亡

Abstract: Objective To study the effects of PIAS3 knocking down on proliferation, cell cycle and apoptosis of human prostate cancer cell line DU145. Methods PIAS3 specific short hairpin RNA(shRNA) expressing plasmid was constructed and named pSilencer4.1/PIAS3.DU145 cell was transfected with pSilencer4.1/PIAS3. The proliferation of DU145 cells was analyzed by MTT assay.Cell cycle and apoptosis of DU145 cells were analyzed by flowcytometry. Results PIAS3 shRNA expressing plasmid was constructed succefully,confirmed by sequencing.Expression of PIAS3 in DU145 was reduced significantly after pSilencer4.1/PIAS3 transfection. MTT assay shown accelerated proliferation after PIAS3 knocking down,and shown dose-effect curve. Flowcytometry shown cells in S phase increased,cells in G0/G1 decreased and percentage of apoptotic cells decreased after PIAS3 knocking down.Conclusion The knocking down of PIAS3 expression could accelerate DU145 cell proliferation and inhibit cell apoptosis.

Key words: Prostate carcinoma, Protein Inhibitor of Activated STAT3, RNA interference, Apoptosis

何旺祝志臻程志强王扬李鸣. PIAS3敲低对前列腺癌细胞增殖和凋亡的影响[J]. 基础医学与临床, 2008, 28(5): 469-472.

. Effects of PIAS3 knocking down on proliferation and apoptosis of prostate carcinoma cell[J]. Basic & Clinical Medicine, 2008, 28(5): 469-472.

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