连接蛋白2和FGF23在房颤介导心肌病兔心房组织中的表达

doi:10.16352/j.issn.1001-6325.2024.02.0199

基础医学与临床 ›› 2024, Vol. 44 ›› Issue (2): 199-203.doi: 10.16352/j.issn.1001-6325.2024.02.0199

连接蛋白2和FGF23在房颤介导心肌病兔心房组织中的表达

郭爽¹, 李树仁^2*, 赵美², 郝潇²

1.河北医科大学研究生学院,河北石家庄 050011;
2.河北省人民医院心内科,河北石家庄 050057

收稿日期:2023-06-07 修回日期:2023-11-27 发布日期:2024-02-05
通讯作者: ^*lsr64@126.com
基金资助:
河北省自然科学基金(C2015307019)

Expression of junctophilin 2 and FGF23 in atrial tissue of rabbits with atrial fibrillation cardiomyopathy

GUO Shuang¹, LI Shuren^2*, ZHAO Mei², HAO Xiao²

1. Graduate School, Hebei Medical University,Shijiazhuang 050011;
2. Department of Cardiology,Hebei General Hospital,Shijiazhuang 050057,China

Received:2023-06-07 Revised:2023-11-27 Published:2024-02-05
Contact: ^*lsr64@126.com

摘要/Abstract

摘要： 目的探究连接蛋白2(JP2)和成纤维细胞生长因子23(FGF23)在房颤介导心肌病(AMC)兔中的表达规律。方法通过左心房快速起搏法建立心房颤动(AF)模型,4周后行超声心动图检查,射血分数下降＞10%纳入AMC组,否则为AF组,对照组只植入起搏器不起搏。最终成功建立AF动物模型11只,其中AF组6只,AMC组5只,对照组6只。超声心动图检测左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、左室射血分数(LVEF)等指标,酶联免疫吸附法(ELISA)检测血清JP2和FGF23水平。处死动物后,取心房组织,Western blot和RT-qPCR检测JP2和FGF23蛋白及mRNA表达。结果与对照组相比,AMC 组左房内径、右房内径、右室内径增大,LVEF 降低,与AF组相比,AMC组LVEF降低,主动脉增宽,右室扩大。与对照组相比,AF组左房心肌细胞FGF23(P＜0.001)、JP2(P＜0.01)的表达均明显增加,而AMC组JP2表达降低(P＜0.001)。与AF组相比,AMC组FGF23和JP2的表达下降。与对照组相比,AF组FGF23和JP2血浆浓度升高,AMC组FGF23水平升高。与AF组相比,AMC组FGF23和JP2血浆浓度偏低。结论在AMC兔模型中,FGF23表达增加,JP2表达下降。

关键词: 心房颤动, 房颤介导心肌病, 连接蛋白2, 成纤维细胞生长因子23

Abstract: Objective To explore the expression of junctophilin 2(JP2)and fibroblast growth factor 23(FGF23)in a rabbit model of atrial fibrillation mediated-cardiomyopathy(AMC). Methods Rabbit models of atrial fibrillation (AF) were developed through rapid atrial stimulation and then divided into three groups: control group (pacemakers implanted without pacing, n=6), AF group (pacing with ejection fraction decrease ＜10%,n=5), and AMC group (pacing with ejection fraction decrease ≥10%, n=6).Echocardiography was performed to detect left ventricular end-diastolic diameter(LVEDD),left ventricular end-systolic diameter(LVESD) and left ventricular ejection fraction(LVEF). JP2 and FGF23 were detected by ELISA method.Western blot and RT-qPCR were conducted to detect protein and mRNA expression of JP2 and FGF23. Results Left atrial diameter, right atrial diameter and right ventricular diameter increased and LVEF decreased in the AMC group as compared with the control group. AMC group had lower LVEF and larger aorta and right ventricle diameter. Compared with the control group, the expression of FGF23(P＜0.001)and JP2(P＜0.01)in left atrial cardiomyocytes was significantly increased in the AF group, while the expression of JP2 was decreased in the AMC group(P＜0.001).AMC group had lower expression of JP2 and FGF23 compared with AF group. Compared to the control group,plasma concentration of JP2 and FGF23 increased in the AF group and FGF23 plasma concentration increased in the AMC group. Plasma concentration of FGF23 and JP2 was lower in AMC group than that in AF group . Conclusions FGF23 expression increased and JP2 expression decreased as found in the rabbit AMC model.

Key words: atrial fibrillation, atrial fibrillation mediated-cardiomyopathy, fibroblast growth factor 23, junctophilin 2

中图分类号:

R541.75

郭爽, 李树仁, 赵美, 郝潇. 连接蛋白2和FGF23在房颤介导心肌病兔心房组织中的表达[J]. 基础医学与临床, 2024, 44(2): 199-203.

GUO Shuang, LI Shuren, ZHAO Mei, HAO Xiao. Expression of junctophilin 2 and FGF23 in atrial tissue of rabbits with atrial fibrillation cardiomyopathy[J]. Basic & Clinical Medicine, 2024, 44(2): 199-203.

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连接蛋白2和FGF23在房颤介导心肌病兔心房组织中的表达

Expression of junctophilin 2 and FGF23 in atrial tissue of rabbits with atrial fibrillation cardiomyopathy

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