支气管哮喘患儿气道微生态与血清免疫因子相关

doi:10.16352/j.issn.1001-6325.2023.09.1439

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (9): 1439-1442.doi: 10.16352/j.issn.1001-6325.2023.09.1439

支气管哮喘患儿气道微生态与血清免疫因子相关

贺雷^*, 颜凯, 邓迎霞

成都市双流区妇幼保健院儿科,四川成都 610200

收稿日期:2022-11-04 修回日期:2023-02-22 出版日期:2023-09-05 发布日期:2023-09-01
通讯作者: ^*helei1124@126.com
基金资助:
2021年成都市医学科研课题基金(2021427)

Airway microecology of children with bronchial asthma is related to serum immune factors

HE Lei^*, YAN Kai, DENG Yingxia

Department of Pediatrics, Chengdu Shuangliu District Maternal and Child Health Hospital, Chengdu 610200, China

Received:2022-11-04 Revised:2023-02-22 Online:2023-09-05 Published:2023-09-01
Contact: ^*helei1124@126.com

摘要/Abstract

摘要： 目的探究支气管哮喘不同时期患儿气道微生态与血清免疫因子的相关性。方法选取2019年4月至2021年5月进入成都市双流区妇幼保健院进行治疗的96例支气管哮喘患儿,其中包括46例缓解期和50例急性发作期,另选取同期进行体检的40名健康小儿作为健康组。对3组小儿采用MiSeq高通量测序平台进行诱导痰16S rRNA基因高通量测序,采用酶联免疫吸附法检测3组小儿的IL-4、IL-10、IL-17和IFN-γ水平。结果支气管哮喘患儿Shannon指数与IL-4、IL-17呈负相关(P＜0.001),Shannon指数与IL-10、IFN-γ呈正相关(P＜0.001);支气管哮喘患儿Ace指数与IL-4、IL-17呈正相关(P＜0.001),Ace指数与IL-10、IFN-γ呈负相关(P＜0.001)。结论支气管哮喘患儿气道微生态存在明显失调,Shannon指数、Ace指数与IL-4、IL-17、IL-10及IFN-γ密切相关。

关键词: 支气管哮喘, Shannon指数, Ace指数

Abstract: Objective To investigate the correlation between 16S rRNA gene high-throughput sequencing of induced sputum and serum immune factors in children with bronchial asthma at different stages. Methods From April 2019 to May 2021, 96 children with bronchial asthma who were treated in Chengdu Shuangliu District Maternal and Child Health Hospital were selected, which covered 46 remission cases and 50 acute cases.In addition, 40 healthy children who underwent physical examination at the same time were selected as the controls group. MiSeq high-throughput sequencing platform was used to sequence the 16S rRNA gene in induced sputum from three groups of children, and the level of IL-4, IL-10, IL-17 and IFN-γ in three groups of children was detected by enzyme-linked immunosorbent assay. Results The results correlation analysis showed that Shannon index was negatively correlated with IL-4 and IL-17 in children with bronchial asthma (P＜0.001), Shannon index was positively correlated with IL-10 and IFN-γ (P＜0.001); Ace index was positively correlated with IL-4 and IL-17 in children with bronchial asthma (P＜0.001). Ace index was negatively correlated with IL-10 and IFN-γ(P＜0.001). Conclusions The airway microecology of children with bronchial asthma is obviously dysregulated.

Key words: bronchial asthma, Shannon index, Ace index

中图分类号:

贺雷, 颜凯, 邓迎霞. 支气管哮喘患儿气道微生态与血清免疫因子相关[J]. 基础医学与临床, 2023, 43(9): 1439-1442.

HE Lei, YAN Kai, DENG Yingxia. Airway microecology of children with bronchial asthma is related to serum immune factors[J]. Basic & Clinical Medicine, 2023, 43(9): 1439-1442.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/10.16352/j.issn.1001-6325.2023.09.1439

http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/Y2023/V43/I9/1439

参考文献

[1]Abdelmohsen G, Mohamed H, Mohsen M, et al. Evaluation of cardiac function in pediatric patients with mild to moderate bronchial asthma in the era of cardiac strain imaging[J]. Pediatr Pulmonol, 2019, 54:1905-1913.
[2]邬奇翰,邱蕾,吴杰. 孟鲁司特钠联合布地奈德福莫特罗粉吸入治疗支气管哮喘急性发作的疗效及对炎症的影响[J]. 广东医学,2022,43:380-383.
[3]张小宁,张向峰,张志英. 儿童支气管哮喘诱导痰MUC5AC和MUC5B水平及临床意义[J]. 医学研究杂志,2021,50:100-103,108.
[4]吕欣,莫碧文.哮喘气道平滑肌细胞表观遗传学调控的研究进展[J].基础医学与临床,2017,37(03):419-421.
[5]Callahan BJ, Wong J, Heiner C, et al. High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution[J].Nucleic Acids Res, 2019, 47:e103.doi: 10.1093/nar/gkz569.
[6]陈菊梅,王红霞,朱青雅,等. 哮喘儿童中痰细胞学与临床表现的相关性[J]. 宁夏医科大学学报,2020,42:1227-1230.
[7]中华医学会儿科学分会呼吸学组,《中华儿科杂志》编辑委员会. 儿童支气管哮喘诊断与防治指南(2016年版)[J]. 中华儿科杂志,2016,54:167-181.
[8]鲍燕敏,谢淦,冯志冠,等. 轻度支气管哮喘儿童的诱导痰菌群特征[J]. 中国微生态学杂志,2020,32:278-282.
[9]马媛媛,钟斌,黄冬平,等. 哮喘患儿血清miR-29b与外周血Th1、Th2细胞失衡的相关性研究[J]. 河北医药,2021,43:1640-1643,1648.
[10]赵宏芳,刘娟. 支气管哮喘患儿外周血单核细胞培养上清液中肿瘤坏死因子-α、白细胞介素-17和免疫球蛋白E水平与Toll样受体4表达的相关性[J]. 新乡医学院学报,2020,37:687-690.
[11]陈思敏,吴秀艳,彭桂英,等. 基于16S rRNA测序研究芍药甘草汤对支气管哮喘小鼠肠道菌群的影响[J]. 北京中医药大学学报,2022,45:492-499.
[12]Almonacid DE, Kraal L, Ossandon FJ, et al. 16S rRNA gene sequencing and healthy reference ranges for 28 clinically relevant microbial taxa from the human gut microbiome[J]. PLoS One, 2017, 12.doi: 10.1371/journal.pone.0276752.

支气管哮喘患儿气道微生态与血清免疫因子相关

Airway microecology of children with bronchial asthma is related to serum immune factors

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	孙莹, 杨治安, 何瑶, 余勤. HMGB1信号通路在支气管哮喘中作用的研究进展[J]. 基础医学与临床, 2023, 43(4): 690-694.
[2]	刘芬, 胡润芳, 茅松, 徐敏, 石文静, 陈凌. 地塞米松减轻过敏性哮喘模型小鼠气道炎性反应[J]. 基础医学与临床, 2023, 43(3): 402-407.