蛋白质精氨酸脱亚胺酶2与4在恶性间皮瘤中的表达及其临床意义

doi:10.16352/j.issn.1001-6325.2023.07.1117

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (7): 1117-1121.doi: 10.16352/j.issn.1001-6325.2023.07.1117

蛋白质精氨酸脱亚胺酶2与4在恶性间皮瘤中的表达及其临床意义

干译涵¹, 沈蔚², 陈伊童¹, 严凯丽¹, 胡帅悦¹, 蒋兆强¹, 应士波^1,*

1.杭州医学院,浙江杭州 310013;
2.慈溪市第三人民医院呼吸内科, 浙江宁波 315324

收稿日期:2022-11-01 修回日期:2022-04-27 发布日期:2023-07-05
通讯作者: ^*shiboying@hmc.edu.cn
基金资助:
国家自然科学基金(81973011); 浙江省基础公益研究计划(LGF21H260010); 国家级大学生创新创业训练计划(202213023010)

Expression of peptidylarginine deiminase 2 and 4 in malignant mesothelioma and its clinical significance

GAN Yihan¹, SHEN Wei², CHEN Yitong¹, YAN Kaili¹, HU Shuaiyue¹, JIANG Zhaoqiang¹, YING Shibo^1,*

1. Hangzhou Medical College, Hangzhou 310013;
2. Department of Respiratory Medicine, the Third People’s Hospital of Cixi, Ningbo 315324, China;

Received:2022-11-01 Revised:2022-04-27 Published:2023-07-05

摘要/Abstract

摘要： 目的探讨恶性间皮瘤(MM)组织中蛋白质精氨酸脱亚胺酶(PAD2和PAD4)的表达及其与瓜氨酸化蛋白的相关性和临床意义。方法回顾性收集2014年至2021年浙江省45例MM病理组织样本,制成组织芯片,利用免疫组织化学法检测癌组织和配对癌旁组织中PAD2与PAD4的表达及其与瓜氨酸化蛋白的相关性,结合生物信息库数据分析其临床意义。结果 PAD2和PAD4在癌组织中的表达显著高于癌旁组织(P<0.05)。PAD2和PAD4的蛋白表达在上皮型恶性间皮瘤、腹膜和胸膜部位的MM中呈正相关(P<0.05)。PAD2与PAD4的mRNA转录水平呈正相关(P<0.05)。PAD2、PAD4的表达与瓜氨酸化蛋白分别呈正相关(P<0.05)。PAD2和PAD4高表达组的总生存期(OS)均较低表达组更短,PAD4高表达组的无进展间隔期(PFI)相对于低表达组更长(P<0.05)。结论 PAD2和PAD4可能与MM进展和预后有关,具有作为生物标志物或治疗靶点的潜在价值。

关键词: 恶性间皮瘤, 蛋白质精氨酸脱亚胺酶2, 蛋白质精氨酸脱亚胺酶4, 瓜氨酸化

Abstract: Objective To investigate the expression of peptidylarginine deiminase 2 and 4 (PAD2 and PAD4) in malignant mesothelioma (MM), their correlation with citrullinated proteins and clinical significance. Methods The pathological samples of 45 cases of MM in Zhejiang province from 2014 to 2021 were collected retrospectively for constructing paraffin-embedded tissue microarray. The expressions of PAD2 and PAD4 in tumor and paired adjacent tissues were detected by immunohistochemistry and the clinical significance was analyzed by utilizing bioinformatics database. Results The expressions of PAD2 and PAD4 in MM tissues were significantly higher than those in adjacent tissues (P<0.05). The protein expression of PAD2 and PAD4 was positively correlated with epithelial mesothelioma, peritoneal mesothelioma and pleural mesothelioma (P<0.05). There was a positive correlation between PAD2 and PAD4 mRNA transcription level (P<0.05). The expressions of PAD2 and PAD4 were positively correlated with citrullinated proteins, respectively (P<0.05). The overall survival(OS) of PAD2 and PAD4 high expression group was shorter than that of low expression group, the progression free interval(PFI) of PAD4 high expression group was longer than that of low expression group (P<0.05). Conclusions PAD2 and PAD4 are potentially involved in the progression and prognosis of MM as biomarkers or therapeutic targets.

Key words: malignant mesothelioma, peptidylarginine deiminase 2, peptidylarginine deiminase 4, citrullination

中图分类号:

干译涵, 沈蔚, 陈伊童, 严凯丽, 胡帅悦, 蒋兆强, 应士波. 蛋白质精氨酸脱亚胺酶2与4在恶性间皮瘤中的表达及其临床意义[J]. 基础医学与临床, 2023, 43(7): 1117-1121.

GAN Yihan, SHEN Wei, CHEN Yitong, YAN Kaili, HU Shuaiyue, JIANG Zhaoqiang, YING Shibo. Expression of peptidylarginine deiminase 2 and 4 in malignant mesothelioma and its clinical significance[J]. Basic & Clinical Medicine, 2023, 43(7): 1117-1121.

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蛋白质精氨酸脱亚胺酶2与4在恶性间皮瘤中的表达及其临床意义

Expression of peptidylarginine deiminase 2 and 4 in malignant mesothelioma and its clinical significance

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