Abstract: Objective To observe the expressions of heat shock 27-associated protein 1 (HSPBAP1) and echinoderm microtubule-associated protein-like 5 (EML5) in cerebrospinal fluid of drug-resistant epilepsy, and to explore the value in early diagnosis of epilepsy. Methods According to the inclusion and exclusion criteria, 79 patients admitted in Department of Neurology, Hubei Xinhua Hospital and the First and Second Affiliated Hospital of Chongqing Medical University were divided into drug-resistant epilepsy group (n = 39) and non-epileptic control group (n = 40). Cerebrospinal fluid (every sample 4 ml) were collected by lumbar puncture specimens, and HSPBAP1 and EML5 were detected by sandwich enzyme-linked immunosorbent assays. SPSS 13.0 software was used for statistical analysis, and P ≤ 0.05 indicated significant differences. Results The expressions of HSPBAP1 and EML5 were 0.17 ± 0.03 and 0.13 ± 0.02 in drug-resistant epilepsy group, while were 0.10 ± 0.03 and 0.08 ± 0.02 in non-epileptic control group. There was significant difference between 2 groups (t = 3.239, P = 0.002; t = 3.294, P = 0.002, respectively). Conclusion The expressions of HSPBAP1 and EML5 were increased in drug-resistant epilepsy patients. This provides a new way for early diagnosis of drug-resistant epilepsy．

Key words: Drug tolerance, Epilepsy, Heat-shock proteins, Microtubule-associated proteins, Cerebrospinal fluid

摘要： 目的  观察热休克蛋白27 相关蛋白1（HSPBAP1）和棘皮动物微管结合蛋白类似蛋白5（EML5）在耐药性癫痫患者脑脊液中的表达变化，探讨其用于耐药性癫痫早期诊断的临床价值。方法  根据纳入标准共选择39 例耐药性癫痫患者和性别、年龄与之相匹配的健康志愿者40 例入组，经腰椎穿刺术采集脑脊液标本，双抗体夹心酶联免疫吸附法检测HSPBAP1 和EML5。结果  耐药性癫痫组患者脑脊液HSPBAP1 和EML5 表达水平分别为（0.17 ± 0.03）和（0.13 ± 0.02），正常对照组为（0.10 ± 0.03）和（0.08 ± 0.02），低于耐药性癫痫组且差异具有统计学意义（t = 3.239，3.294；均P = 0.002）。结论  耐药性癫痫患者脑脊液HSPBAP1和EML5 表达水平明显升高，可为耐药性癫痫的诊断提供新的途径，有助于耐药性癫痫的早期诊断。

关键词: 药物耐受性, 癫痫, 热休克蛋白质类, 微管相关蛋白质类, 脑脊髓液